Alignments for a candidate for etoh-dh-c in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Alignments for a candidate for etoh-dh-c in Cupriavidus basilensis 4G11

Align quinoprotein decanol dehydrogenase; EC 1.1.-.- (characterized)
to candidate RR42_RS05285 RR42_RS05285 quinonprotein alcohol dehydrogenase

Query= CharProtDB::CH_107432
         (595 letters)



>FitnessBrowser__Cup4G11:RR42_RS05285
          Length = 582

 Score =  307 bits (786), Expect = 9e-88
 Identities = 189/518 (36%), Positives = 279/518 (53%), Gaps = 36/518 (6%)

Query: 46  TNGLGVQGQRYSPLDLLNANNVKELRPVWAFSFGGEKQRGQQAQPLIKDGVMYLTGSYSR 105
           +NG  VQ  RY P   +N  NV +L+P  AF F        +  P++ +GVM+LT SY+ 
Sbjct: 72  SNGSYVQ-TRYYPGSQINTRNVAKLKP--AFLFQTAVLESMETAPIVVNGVMFLTTSYNH 128

Query: 106 VFAVDARTGKKLWQYDARLPDDIRPCCDVINRGVALYGDLVFFGTLDAKLVALNKDTGKV 165
           V+A+DA TGK+ W Y  ++      CC   NRGVA+ GD VF GTLDA+LVAL+  TGK+
Sbjct: 129 VYAIDAVTGKEFWHYKHKMGPITTFCCGPNNRGVAVSGDRVFMGTLDARLVALDARTGKL 188

Query: 166 VWSKKVADHKEGYSISAAPMIVNGKLITGVAGGEFGVVGKIQAYNPENGELLWMRPTV-- 223
           +W  ++AD + GYS + AP++V+ K++ G  GGE+G+ G ++AY+  +G+LLW   T+  
Sbjct: 189 LWETQIADPELGYSETMAPVVVDDKILIGTNGGEYGIRGFLKAYSATDGKLLWTFYTIPE 248

Query: 224 EGHMG-YVYKD--GKAIENGISGGE---AGKTWPGDLWKTGGAAPWLGGYYDPETNLILF 277
           +GH G +  +D  G+ ++  I+  +   A K   GD +K  G   W+    D +  + +F
Sbjct: 249 KGHEGVWATRDATGRDMKRDIAAEKKQLADK--GGDFYKALGGGVWMPPAIDRKNRMAIF 306

Query: 278 GTGNPAP-WNSHLRPGDNLYSSSRLALNPDDGTIKWHFQSTPHDGWDFDGVNELISFNYK 336
             GNP+P     +RPGDNLY+ S +A++ D G  KWH Q   HD WD D V+  I  + +
Sbjct: 307 VVGNPSPDLYGAIRPGDNLYTDSMVAVDLDTGAYKWHSQYIAHDVWDLDAVSPPILVDVR 366

Query: 337 D-GGKEVKAAATADRNGFFYVLDRTNGKFIRGFPFVDKITWATGLDKDGRPIYNDASRPG 395
           D  G+ +       + G  YV DR +G+ IR               +   P  +  + P 
Sbjct: 367 DKNGQMIPGVIHGGKTGHVYVHDRRDGRLIR-------------FSQAMIPQEDMWTLPT 413

Query: 396 APGSEAKGSSVFVAPAFLGAKNWMPMAYNKDTGLFYVPSNEWGMDIWNEGIAYKKGAAFL 455
           A G+        + P   G   W PMA+N  T + Y  +    M    E   Y  G  +L
Sbjct: 414 ANGAR-------MLPGANGGVEWSPMAFNPKTRMAYAANLHQPMTYQVEASPYPGGKLWL 466

Query: 456 GAGFTIKPLNEDYIGVLRAIDPISGKEVWRHKNYAPLWGGVLTTKGNLVFTGTPEGFLQA 515
           G  F   P    + G L A++  +GK VW +K   PL GGVL T G LVF G   G  +A
Sbjct: 467 GGAFKTIPAEAQW-GKLSAVNIDTGKVVWDYKTDQPLIGGVLATAGGLVFNGEGNGLFRA 525

Query: 516 FNAKTGDKVWEFQTGSGVLGSPVTWEMDGEQYVSVVSG 553
           F+A TG K+WE+Q G+GV    V++ + G+QY++V +G
Sbjct: 526 FDAATGKKLWEYQCGAGVNAPAVSYMVGGKQYIAVAAG 563


Lambda     K      H
   0.317    0.137    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1316
Number of extensions: 96
Number of successful extensions: 17
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 595
Length of database: 582
Length adjustment: 37
Effective length of query: 558
Effective length of database: 545
Effective search space:   304110
Effective search space used:   304110
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources