Align ABC transporter related; Flags: Precursor (characterized, see rationale)
to candidate RR42_RS32900 RR42_RS32900 sugar ABC transporter ATPase
Query= uniprot:B2T9V9 (510 letters) >FitnessBrowser__Cup4G11:RR42_RS32900 Length = 502 Score = 264 bits (674), Expect = 6e-75 Identities = 164/490 (33%), Positives = 265/490 (54%), Gaps = 13/490 (2%) Query: 5 MSELTSSVPVVEALEVTKRFGSTAALNDVSIRVMPGESHALVGRNGAGKSTLVSILTGLR 64 M+ +T P+ E + K F AL+DVS + PGE H L+G NGAGKS+L+ +L G+ Sbjct: 1 MNAITEITPLFEMRGICKNFPGVKALDDVSFAIYPGEVHMLLGENGAGKSSLMKVLCGVY 60 Query: 65 KPDTGEVRFSGAAAPSIADRDAWRERVACVYQHSTIIRDLSVAENLFINRQPLRG---GV 121 D GE G+ + D +A ++Q +++ L +A+N+F+ R+P RG G Sbjct: 61 VADAGEFYHDGSPVAITSPADTMGLGIAVIFQEFSLVPYLDIAQNIFLGREP-RGRIPGS 119 Query: 122 IDWQAMRRDARALLDHWKIDVREDARAGDLSVEARQLVEIARALSYGARFIILDEPTAQL 181 +D M +AR +LD ++V L V +Q+VEIA+ALS AR ++LDEPTA L Sbjct: 120 VDAARMHAEARRILDILGMEVSTRTPVHRLGVAQQQMVEIAKALSQNARILVLDEPTAAL 179 Query: 182 DGDEIKRLFRRISELQREGVTFLFISHHLQEVYEICQAVTVLRDARHI-VSAPVSALPRE 240 E ++LF I+ L+ +GV+ ++ISH + EV+ + +T+LRD R + P A P + Sbjct: 180 SDRETEKLFAVIARLKADGVSMIYISHRMAEVFALGDRITILRDGRKVGACLPGDATP-D 238 Query: 241 QLIEAMTGERGGLAVADAAARGALPADTAVALELKELTG-ADYEGVSFTVKRGEVVGLTG 299 +L+ M G + + ++ + R A P + VAL+++ ++ + ++ V+ GE+VGL G Sbjct: 239 ELVARMVGRK--VDMSYSRERSAQPGE--VALDVRNVSADSGIADINLQVRAGEIVGLAG 294 Query: 300 ATSSGRTSVAEAIAGLRAAKRGTISVDGAILPPGDVPASLAHGIGCVPKDRHHEGLVLTQ 359 SGR+ VA A+ G ++G I + G L G A G +P+ R EGL L + Sbjct: 295 LVGSGRSEVARAVFGADPIRQGEIYIFGKRLTGGPDRAR-ELGAALIPESRKSEGLALIR 353 Query: 360 SVAENASMTIARVLGKFGIAAPAKKNAFGQKMIDALGIVAQGPEHVVSGLSGGNQQKVVM 419 +V +N + R K A ++ I L I + LSGGNQQK+V+ Sbjct: 354 TVRDNLLLAGLRRAFPARWYRADKAEALAEREIARLRIATPDGNQLAQFLSGGNQQKIVI 413 Query: 420 ARALATNPNVLVLIDPTAGVDVKSKEALLSVVDRVREEGKAVLVVSGELDD-LRTCDRVL 478 + L + + +PT G+DV +K + +++D + ++G VL++S EL + + CDR Sbjct: 414 GKWLVAEAKLFIFDEPTRGIDVGAKAEIFALIDSLVKQGAGVLLISSELPEIINVCDRTY 473 Query: 479 VMFRGRVAAE 488 VM GR+A E Sbjct: 474 VMRGGRIAGE 483 Score = 53.9 bits (128), Expect = 1e-11 Identities = 58/242 (23%), Positives = 103/242 (42%), Gaps = 10/242 (4%) Query: 10 SSVPVVEALEVTKRFGSTAALNDVSIRVMPGESHALVGRNGAGKSTLVSILTGLRKPDTG 69 S+ P AL+V + + + + D++++V GE L G G+G+S + + G G Sbjct: 258 SAQPGEVALDV-RNVSADSGIADINLQVRAGEIVGLAGLVGSGRSEVARAVFGADPIRQG 316 Query: 70 EVRFSGAAAPSIADRDAWRERVACVYQHSTIIRDLS----VAENLFINRQPLRGGVIDWQ 125 E+ G DR RE A + S L+ V +NL + ++ Sbjct: 317 EIYIFGKRLTGGPDRA--RELGAALIPESRKSEGLALIRTVRDNLLLAGLRRAFPARWYR 374 Query: 126 AMRRDARALLDHWKIDVRE---DARAGDLSVEARQLVEIARALSYGARFIILDEPTAQLD 182 A + +A A + ++ + + A LS +Q + I + L A+ I DEPT +D Sbjct: 375 ADKAEALAEREIARLRIATPDGNQLAQFLSGGNQQKIVIGKWLVAEAKLFIFDEPTRGID 434 Query: 183 GDEIKRLFRRISELQREGVTFLFISHHLQEVYEICQAVTVLRDARHIVSAPVSALPREQL 242 +F I L ++G L IS L E+ +C V+R R + + E++ Sbjct: 435 VGAKAEIFALIDSLVKQGAGVLLISSELPEIINVCDRTYVMRGGRIAGEVAHAEMTEERI 494 Query: 243 IE 244 ++ Sbjct: 495 LQ 496 Lambda K H 0.318 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 568 Number of extensions: 32 Number of successful extensions: 10 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 510 Length of database: 502 Length adjustment: 34 Effective length of query: 476 Effective length of database: 468 Effective search space: 222768 Effective search space used: 222768 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory