Aligments for a candidate for edd in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Aligments for a candidate for edd in Cupriavidus basilensis 4G11

Align phosphogluconate dehydratase (characterized)
to candidate RR42_RS18585 RR42_RS18585 dihydroxy-acid dehydratase

Query= CharProtDB::CH_024239
         (603 letters)



>FitnessBrowser__Cup4G11:RR42_RS18585
          Length = 577

 Score =  186 bits (471), Expect = 3e-51
 Identities = 154/513 (30%), Positives = 242/513 (47%), Gaps = 45/513 (8%)

Query: 68  IAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVGQVAGGVPAMCDGVTQGQDGME-LSL 126
           I I  +Y+++   +  +    E ++  + EA       GG P     ++ G+  +   ++
Sbjct: 47  IGICNTYSELTPCNSHFRTLAEQVKIGVWEA-------GGFPLEFPVMSLGETMLRPTAM 99

Query: 127 LSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPGLTMAALSFGHLPAVFVPSGPMASGL- 185
           L R + +M     +  N  DG + L  CDK  P L M A S   LP + V  GPM SG  
Sbjct: 100 LFRNLASMDVEESIRGNPIDGVVLLMGCDKTTPALMMGAASCD-LPTIGVSGGPMLSGKF 158

Query: 186 ------PNKEKVRIRQLYAEGKVDRMALLESEAASYHAPGTCTFYGTANTNQMVVEFMGM 239
                    +  ++ +    G++ +    E+E+  + + G C   GTA+T   +VE +GM
Sbjct: 159 RGGELGSGTDVWKMSEQVRAGQMSQEDFFEAESCMHRSHGHCMTMGTASTMASMVEALGM 218

Query: 240 QLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGG 299
            LPG++ +      R+ L  A+ R++  M  +    + + K++      N I    A GG
Sbjct: 219 SLPGNAAIPAVDGRRNVLARASGRRIVEMVKDN---LVMSKILTRDAFENAIRVNAAIGG 275

Query: 300 STNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRE 359
           STN  +HL+A+A   G+++  +D+  L   +P +  L P+G   +  F  AGG+P ++RE
Sbjct: 276 STNAVIHLLAIAGRIGVELKLEDWDALGHELPCLLDLQPSGRHLMEDFYYAGGLPAVIRE 335

Query: 360 LLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAE-KSLDSNVIASFEQPFSHHG 418
           L    +L  D  TV               NG+  W    +  + +  VI +F  PF  + 
Sbjct: 336 L--ESVLARDALTV---------------NGKTLWDNCKDAPNWNREVIHAFGAPFKANA 378

Query: 419 GTKVLSGNL--GRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRD--CVVVV 474
           G  VL GNL    AV+K SA           AVVFE+   +    +   LD D  CV+V+
Sbjct: 379 GIAVLRGNLCPDGAVIKPSAATPALLKHTGRAVVFENSEHMHQRLDDESLDVDETCVLVL 438

Query: 475 RHQGPKA-NGMPELHKLMPPLGVLLDRCFKIALVTDGRLSG-ASGKVPSAIHVTPEAYDG 532
           ++ GP+   GM E   +  P  VL      +  V+D R+SG A G V   +HV PEA  G
Sbjct: 439 KNCGPRGYPGMAEAGNMPLPPKVLRKGITDMVRVSDARMSGTAYGTV--VLHVAPEAAAG 496

Query: 533 GLLAKVRDGDIIRVNGQTGELTLLVDEAELAAR 565
           G LA V+DGD++ ++    +L L VDEAEL  R
Sbjct: 497 GPLALVKDGDMVELDVPMRKLHLHVDEAELQRR 529


Lambda     K      H
   0.318    0.134    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 845
Number of extensions: 48
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 603
Length of database: 577
Length adjustment: 37
Effective length of query: 566
Effective length of database: 540
Effective search space:   305640
Effective search space used:   305640
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources