Alignments for a candidate for gdhA in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Alignments for a candidate for gdhA in Cupriavidus basilensis 4G11

Align NAD-specific glutamate dehydrogenase; NAD-GDH; EC 1.4.1.2; Surface-associated protein PGAG1 (uncharacterized)
to candidate RR42_RS33640 RR42_RS33640 glutamate dehydrogenase

Query= curated2:B2RKJ1
         (445 letters)



>FitnessBrowser__Cup4G11:RR42_RS33640
          Length = 447

 Score =  488 bits (1256), Expect = e-142
 Identities = 237/433 (54%), Positives = 317/433 (73%), Gaps = 3/433 (0%)

Query: 13  KHPGESEFLQAVKEVLLSVEEVYNQHPEFEKNGIIERIVEPDRVFTFRVPWVDDQGKVQV 72
           + P + EFLQAVKEV++++     QHP + +  ++ER+VEP+RV  FRV W DD+G+VQV
Sbjct: 16  RDPDQPEFLQAVKEVMMTLWPFVEQHPRYGEQALLERLVEPERVIQFRVAWTDDRGQVQV 75

Query: 73  NIGYRVQFNNAIGPYKGGIRFHPSVNLSILKFLGFEQMFKNALTTLPMGGGKGGADFSPK 132
           N G+RVQ ++AIGPYKGG+R HP+VNLS+LKFLGFEQ  KNALTTLPMGGGKGG+DF PK
Sbjct: 76  NRGFRVQHSSAIGPYKGGMRLHPTVNLSVLKFLGFEQTLKNALTTLPMGGGKGGSDFDPK 135

Query: 133 GKSEAEIMRFCQSFMTELWRNIGPDTDIPAGDIGVGGREVGYMFGMYKKLAREHTGTLTG 192
           GKS+ E+MRFCQ+ MTEL+R++GPDTD+PAGDIGVG REVG+M GM KKL+       TG
Sbjct: 136 GKSDGEVMRFCQALMTELYRHLGPDTDVPAGDIGVGAREVGFMAGMMKKLSNSSASVFTG 195

Query: 193 KGFEFGGSRLRPESTGFGAVYFVQNMCKQNGVDYKGKTLAISGFGNVAWGVAQKATELGI 252
           KG  FGGS +RPE+TG+G VYF Q M  + G D+ G  + +SG GNVA   A+KA ELG 
Sbjct: 196 KGMAFGGSLMRPEATGYGTVYFAQEMLHRRGRDFSGLRVLLSGSGNVAQYAAEKAIELGA 255

Query: 253 KVVTISGPDGYVYDPDGINTPEKFRCMLDLRDSGNDVVSDYVKRFPNAQFFPGKKPWEQK 312
           KV+T+S   G ++ P G+N+ E+   ++  ++     ++++     +  F  G+ PW   
Sbjct: 256 KVLTVSDSGGVLHYPKGMNS-EQLSALMAFKNEARGRLAEFAAE-QDLHFEAGRTPWHVP 313

Query: 313 VDFAMPCATQNEMNLEDAKTLHKNGVTLVAETSNMGCTAEASEYYVANKMLFAPGKAVNA 372
            D A+PCATQNE++  DA+ L  NGV  VAE +NM  T +A + ++A ++L+APGKA NA
Sbjct: 314 ADVALPCATQNELDGNDAERLLANGVFCVAEGANMPATLDAVDRFLAARILYAPGKASNA 373

Query: 373 GGVSCSGLEMTQNAMHLVWTNEEVDKWLHQIMQDIHEQCVTYG-KDGNYIDYVKGANIAG 431
           GGV+ SGLEM+QNAM + W + EVD+ LH IM+DIH  C+ YG K   +I+YV+GAN+AG
Sbjct: 374 GGVATSGLEMSQNAMRMSWHHAEVDEKLHAIMKDIHGNCIHYGQKADGFINYVEGANVAG 433

Query: 432 FMKVAKAMVAQGV 444
           F+KVA AM+AQGV
Sbjct: 434 FVKVADAMLAQGV 446


Lambda     K      H
   0.319    0.137    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 658
Number of extensions: 25
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 445
Length of database: 447
Length adjustment: 32
Effective length of query: 413
Effective length of database: 415
Effective search space:   171395
Effective search space used:   171395
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources