Alignments for a candidate for glpO in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Alignments for a candidate for glpO in Cupriavidus basilensis 4G11

Align Alpha-glycerophosphate oxidase; EC 1.1.3.21; Exported protein 6; Glycerol-3-phosphate oxidase (uncharacterized)
to candidate RR42_RS16760 RR42_RS16760 FAD-dependent oxidoreductase

Query= curated2:P35596
         (608 letters)



>FitnessBrowser__Cup4G11:RR42_RS16760
          Length = 559

 Score =  194 bits (492), Expect = 1e-53
 Identities = 167/566 (29%), Positives = 256/566 (45%), Gaps = 87/566 (15%)

Query: 8   RELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLETGLIEMQDFAEGTSSRSTKLVHG 67
           R+  + ++   T D+LI+GGG+TGA  AL A+  G    L+E  DFA GTSS+S+K+VHG
Sbjct: 26  RQAQLDRLGSETFDILIVGGGVTGAYAALDASLRGYRVALVEKNDFASGTSSKSSKMVHG 85

Query: 68  GLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKSDPMLLPVYDEDGATFSLFRLKVAMD-- 125
           GLRY++Q ++ +V  ++ ER  +++ A H+ +  P L PV ++DG      RL  A +  
Sbjct: 86  GLRYIEQGNLGLVRHSLLERQRLRRNARHLVQRLPFLFPVMEKDGVFDK--RLSKAFESL 143

Query: 126 --LYDLLAGVSNTPAANKVLSKDQVLERQPNLKKEGLVGGGVYLDFRNNDARLVIENIKR 183
              YD LAG       ++ L+K +VL   P   +E L+GG +Y D R +DARL +   + 
Sbjct: 144 LWTYD-LAGGWREGILHQKLTKAEVLSHCPTFNEENLLGGFMYFDARVDDARLTLNIART 202

Query: 184 ANQDGALIANHVKAEGFLFDESGKITGVVARDLLTDQVFEIKARLVINTTGPWSDKVRNL 243
           A   GA + NH K      +  GK+ G +      D+    +A +V+  TG W       
Sbjct: 203 AAFHGAAVVNHAKVVEITRNGHGKVDGAIIH--AGDREIRARAGVVVMATGVWLRDWTGR 260

Query: 244 SNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYFDTGLGDGRMVFVLPRENKTYFGTTDTD 303
                +   +RP KGVH+ +   K++    V      G  R   +    N +Y GTTD D
Sbjct: 261 KKGEEKTLHIRPAKGVHVAIPWLKVRNDCTVTIPVP-GRNRRATITRWGNVSYLGTTDED 319

Query: 304 YTGDLEHPKVTQEDVDYLLGIVNNRFPESNITIDDIESSWAGLRPLIAGNSASDYNGGNN 363
           Y GDL++   T+E++D+LL        ++++  +D+  S AG RPL+A        GG  
Sbjct: 320 YEGDLDNVCCTREELDFLLDGARWAL-KTDLQAEDVLGSIAGCRPLVAP------PGG-- 370

Query: 364 GTISDESFDNLIATVESYLSKEKTREDVESAVSKLESSTSEKHLDPSAVSRGSSLDRDDN 423
                                 KT E                      + R   +    +
Sbjct: 371 ----------------------KTLE----------------------IKRNHEIHTAAD 386

Query: 424 GLLTLAGGKITDYRKMAEGAMERVVDILKAEFDRSFKLINSKTYPVSGGELNPANVDSEI 483
           GL+T+ GGK+T  R MAE  ++    +L            ++    S   L  A  D++ 
Sbjct: 387 GLVTIVGGKLTTSRHMAEQTIDAAQKVLGQ---------RNRCQTKSAYLLGAAGYDAQ- 436

Query: 484 EAFAQLGVSRGLDSKEAHYLANLYGSNAPKVFAL--AHSLEQAP---GLSLADTLSLHYA 538
                +  S GL +    +L   YG+ A  V  +  A +  QAP   GL   +   + YA
Sbjct: 437 ----AIVASGGLSA----HLGERYGTEARFVSDILQADARLQAPIVEGLPYTEA-EIVYA 487

Query: 539 MRNELTLSPVDFLLRRTNHMLFMRDS 564
            R+EL  S  D L RR    L  RD+
Sbjct: 488 ARHELAGSVDDVLSRRIRARLMARDA 513


Lambda     K      H
   0.314    0.133    0.368 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 636
Number of extensions: 30
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 608
Length of database: 559
Length adjustment: 36
Effective length of query: 572
Effective length of database: 523
Effective search space:   299156
Effective search space used:   299156
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources