Alignments for a candidate for hutH in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Alignments for a candidate for hutH in Cupriavidus basilensis 4G11

Align Histidine ammonia-lyase; Histidase; EC 4.3.1.3 (uncharacterized)
to candidate RR42_RS01710 RR42_RS01710 tyrosine ammonia-lyase

Query= curated2:Q67JH4
         (507 letters)



>FitnessBrowser__Cup4G11:RR42_RS01710
          Length = 532

 Score =  373 bits (958), Expect = e-108
 Identities = 228/523 (43%), Positives = 311/523 (59%), Gaps = 25/523 (4%)

Query: 3   AVEL-GAHLTLPEVVAVARHGARVVLTPEVRQRVARASEMVERLVRERRPVYGITTGFGK 61
           AVE+ G  LT   +  VA+      L    R  VA A    E L     P+YG++TGFG+
Sbjct: 5   AVEVDGQQLTAAALAQVAQGRRPASLDQAARAHVADARARFEELASSNIPIYGVSTGFGE 64

Query: 62  FSDVPISAEQTEALQRNLLMSHACAVGEPLAAEVVRAMLLLRAQALSRGHSGIRAETLEM 121
                +  E   ALQ NLL SH   VG     E VRAM++ RA AL+RG+S +R   +E 
Sbjct: 65  LVHNWVDIEHAGALQENLLRSHCAGVGPLFGREEVRAMMVARANALARGYSAVRPLVIEQ 124

Query: 122 LVAFLNLGLTPVVPEQGSLGASGDLAPLAHMSLPLIGLGEAVV-NGERLSGAEALQRVGL 180
           L+ ++  G+TP VP+ GSLGASGDLAPL+H+++ LIG G+ ++ +G      + L+  G+
Sbjct: 125 LLRYVEAGITPAVPQVGSLGASGDLAPLSHIAITLIGEGKVLLADGTTAPTGQVLREHGI 184

Query: 181 RPLTLTAKEGLALINGTQAMTALGSLGLHDAQVLLKTADIAAAMTAEALGAIPAAWDPRV 240
            P+ L+ KEGLALINGT AMT +  L L   +  +  A+I AA+  E L A   A+    
Sbjct: 185 EPIKLSHKEGLALINGTSAMTGMACLLLETMRAQVTQAEIIAALALEGLNASADAFMTHG 244

Query: 241 QAL-RLHTGQQAAARNLRRLTEGS-RLTTR---PGQMRT--------------QDPYTLR 281
             + + H GQ  +A NLR L  GS RL+      G+M+T              Q  YTLR
Sbjct: 245 HDIAKPHPGQVQSAANLRALLAGSTRLSGHGQLAGEMKTRAGDEKNTGTGVFIQKAYTLR 304

Query: 282 CLPQVHGASRTAIEHVAQVLDWEMNAVTDNPLLFPDDDEVISGGNFHGQPVALALDYLAI 341
           C+PQV GA R  + H A V++ E+N+  DNPL F DD E+  GGNFHGQ VA A+D+LAI
Sbjct: 305 CIPQVLGAVRDTLGHCATVVERELNSSNDNPLFF-DDGELFHGGNFHGQQVAFAMDFLAI 363

Query: 342 AVAELGDIAERRIERLVNPQL-SGLPAFL-TRNGGVHSGLMITQYTAASLVSENKVLAHP 399
           A  +LG ++ERR+ RL++P L +GLPAFL   N G+  G    QY A +L++EN+ +  P
Sbjct: 364 AATQLGVVSERRLNRLLSPHLNNGLPAFLAAANEGLSCGFAGAQYPATALIAENRTICSP 423

Query: 400 ASVDSIPSSANQEDHVSMGTTAARKARQVIANVRRVLAIELLCAAQALEFVGP-ERLAPA 458
           AS+ S+PS+ + +D VSMG   AR AR++I N + +LA+ELL A QA E  G  E L+PA
Sbjct: 424 ASIQSVPSNGDNQDVVSMGLIGARNARRIIDNNQYILAVELLAACQAAELAGAVESLSPA 483

Query: 459 TRAAYAAIRERVAPLSGDRVLAPDIEALAELVHNGELVAAVEA 501
            RA +A +RERV  L+ DR +  DIEA+A  +  G LV  V A
Sbjct: 484 GRAVFAFVRERVPFLAMDRYMTDDIEAIAAQLRAGALVGVVRA 526


Lambda     K      H
   0.318    0.132    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 663
Number of extensions: 39
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 507
Length of database: 532
Length adjustment: 35
Effective length of query: 472
Effective length of database: 497
Effective search space:   234584
Effective search space used:   234584
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources