Align Histidine ammonia-lyase (EC 4.3.1.3) (characterized)
to candidate RR42_RS14640 RR42_RS14640 histidine ammonia-lyase
Query= reanno::pseudo6_N2E2:Pf6N2E2_3799 (510 letters) >FitnessBrowser__Cup4G11:RR42_RS14640 Length = 510 Score = 823 bits (2126), Expect = 0.0 Identities = 422/510 (82%), Positives = 458/510 (89%) Query: 1 MTALNLIPGQLSLAQLRDIYQQPVTLSLDASASAQIEASVACVEQILAENRTAYGINTGF 60 M ALNL PGQL+LAQLR Y QP+ +SLD A+ I ASVACVE I+AE RTAYGINTGF Sbjct: 1 MNALNLKPGQLTLAQLRQAYLQPLRVSLDGGAAEAIAASVACVENIVAEGRTAYGINTGF 60 Query: 61 GLLASTRIASEDLENLQRSLVLSHAAGVGEPISDALVRLVMVLKVNSLSRGFSGIRRQVI 120 GLLA TRIA EDLENLQRSLVLSHAAGVGEP+ DA+VRL+M+LK+NSL+RGFSGIRR+VI Sbjct: 61 GLLAQTRIAREDLENLQRSLVLSHAAGVGEPLDDAMVRLIMLLKINSLARGFSGIRRKVI 120 Query: 121 DALIALINAEVYPHIPLKGSVGASGDLAPLAHMSLVLLGEGKARYKGEWLPAVEALKVAG 180 DALIAL+NAEVYPHIPLKGSVGASGDLAPLAHMSLVLLGE +ARY+GEWLPA +AL AG Sbjct: 121 DALIALVNAEVYPHIPLKGSVGASGDLAPLAHMSLVLLGESRARYQGEWLPARQALARAG 180 Query: 181 LAPLTLAAKEGLALLNGTQVSTAFALRGLFEGEDLFAGALALGGLTVEAVLGSRSPFDAR 240 L PLTLAAKEGLALLNGTQVSTA+ALRGLFE EDLFA A G L+VEA+LGSR+PFDAR Sbjct: 181 LEPLTLAAKEGLALLNGTQVSTAYALRGLFEAEDLFAAATVCGSLSVEAMLGSRAPFDAR 240 Query: 241 IHAARGQKGQIDAAAAYRDLLGERSEVSDSHQNCDKVQDPYSLRCQPQVMGACLTQFRQA 300 IHA RGQ+GQIDAAA YR LL + SE++ SH CDKVQDPYSLRCQPQVMGACLTQ RQA Sbjct: 241 IHAVRGQRGQIDAAATYRGLLTDTSELARSHAQCDKVQDPYSLRCQPQVMGACLTQLRQA 300 Query: 301 AEVLVIEANAVSDNPLVFAAEGDVISGGNFHAEPVAMAADNMALAIAEIGSLSERRISLM 360 AEVL +EANAVSDNPLVFAAEGDVISGGNFHAEPVAMAADN+ALAIAEIGSLSERRISLM Sbjct: 301 AEVLEVEANAVSDNPLVFAAEGDVISGGNFHAEPVAMAADNLALAIAEIGSLSERRISLM 360 Query: 361 MDKHMSQLPPFLVANGGVNSGFMIAQVTAAALASENKALSHPHSVDSLPTSANQEDHVSM 420 MD+HMSQLPPFLVANGGVNSGFMIAQVTAAALAS+NKAL+HPHSVDSLPTSANQEDHVSM Sbjct: 361 MDRHMSQLPPFLVANGGVNSGFMIAQVTAAALASDNKALAHPHSVDSLPTSANQEDHVSM 420 Query: 421 APAAGKRLWEMAENTRGILAVEWLAACQGLDLRGGLKTSTKLERARGLLRAQVPFYEKDR 480 AP AGKRLW MAEN RGILAVEWL ACQGLD R GLK+S KLE+AR LLRA+VP+YEKDR Sbjct: 421 APNAGKRLWAMAENVRGILAVEWLGACQGLDFREGLKSSPKLEQARSLLRAEVPYYEKDR 480 Query: 481 FFAPDINAASELLASRCLNELVTAQLLPSL 510 FFAPDI AS LLA+ LN L+ A+LLPSL Sbjct: 481 FFAPDIETASRLLAAGTLNALLPARLLPSL 510 Lambda K H 0.318 0.132 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 770 Number of extensions: 10 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 510 Length of database: 510 Length adjustment: 34 Effective length of query: 476 Effective length of database: 476 Effective search space: 226576 Effective search space used: 226576 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
Align candidate RR42_RS14640 RR42_RS14640 (histidine ammonia-lyase)
to HMM TIGR01225 (hutH: histidine ammonia-lyase (EC 4.3.1.3))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01225.hmm # target sequence database: /tmp/gapView.9276.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01225 [M=506] Accession: TIGR01225 Description: hutH: histidine ammonia-lyase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.4e-226 737.5 4.0 3.8e-226 737.3 4.0 1.0 1 lcl|FitnessBrowser__Cup4G11:RR42_RS14640 RR42_RS14640 histidine ammonia-l Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Cup4G11:RR42_RS14640 RR42_RS14640 histidine ammonia-lyase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 737.3 4.0 3.8e-226 3.8e-226 2 504 .. 5 508 .. 4 510 .] 0.99 Alignments for each domain: == domain 1 score: 737.3 bits; conditional E-value: 3.8e-226 TIGR01225 2 vldgesltledleavarekarvelsaaaeeavaksravieeivaedktvYGvntGFGklasvkidkedl 70 l++++ltl++l++ + + rv+l+ a+ea+a+s a++e+ivae +t YG+ntGFG la+++i++edl lcl|FitnessBrowser__Cup4G11:RR42_RS14640 5 NLKPGQLTLAQLRQAYLQPLRVSLDGGAAEAIAASVACVENIVAEGRTAYGINTGFGLLAQTRIAREDL 73 58999**************************************************************** PP TIGR01225 71 aeLqrnlvrsHaaGvGepleeevvRallvlrlnslakGysgvraevlellvallnaevlPvvpekGsvG 139 ++Lqr+lv+sHaaGvGepl++++vR++++l++nsla+G+sg+r++v+++l+al+naev+P++p kGsvG lcl|FitnessBrowser__Cup4G11:RR42_RS14640 74 ENLQRSLVLSHAAGVGEPLDDAMVRLIMLLKINSLARGFSGIRRKVIDALIALVNAEVYPHIPLKGSVG 142 ********************************************************************* PP TIGR01225 140 asGDLAPLahlalvliGeGeaefegevmdaaeaLaaaglePvtlkakEGlALinGtqlmtalavlalvd 208 asGDLAPLah++lvl+Ge a+++ge+++a +aLa+agleP+tl+akEGlAL+nGtq++ta+a+++l++ lcl|FitnessBrowser__Cup4G11:RR42_RS14640 143 ASGDLAPLAHMSLVLLGESRARYQGEWLPARQALARAGLEPLTLAAKEGLALLNGTQVSTAYALRGLFE 211 ********************************************************************* PP TIGR01225 209 aekllesadiaaalsleallgtskafdpdihevrphrgqiavaarlrella.gseiaeshkdedrvqDa 276 ae+l ++a+++++ls+ea+lg++++fd++ih+vr++rgqi+ aa+ r ll+ se+a+sh ++d+vqD+ lcl|FitnessBrowser__Cup4G11:RR42_RS14640 212 AEDLFAAATVCGSLSVEAMLGSRAPFDARIHAVRGQRGQIDAAATYRGLLTdTSELARSHAQCDKVQDP 280 *************************************************9978**************** PP TIGR01225 277 YslRciPqvhGavldtldqvkevlaiElnsatDnPlvfadegevvsgGnFHgepvAlaldflaiaiael 345 YslRc+Pqv+Ga+l++l+q++evl++E+n+++DnPlvfa+eg+v+sgGnFH+epvA+a+d+la+aiae+ lcl|FitnessBrowser__Cup4G11:RR42_RS14640 281 YSLRCQPQVMGACLTQLRQAAEVLEVEANAVSDNPLVFAAEGDVISGGNFHAEPVAMAADNLALAIAEI 349 ********************************************************************* PP TIGR01225 346 gaiseRRierlldpalseLppFLaedsGlnsGlmiaqytaAaLvsenkaLahPasvDsiptsanqEDHv 414 g++seRRi++++d+++s+LppFL++++G+nsG+miaq+taAaL+s+nkaLahP+svDs+ptsanqEDHv lcl|FitnessBrowser__Cup4G11:RR42_RS14640 350 GSLSERRISLMMDRHMSQLPPFLVANGGVNSGFMIAQVTAAALASDNKALAHPHSVDSLPTSANQEDHV 418 ********************************************************************* PP TIGR01225 415 smgaaaarkllevvenvrrviaiEllaaaqglefrkaektaaelekvyeavRevveeleeDRvlapDle 483 sm+ a+++l+ ++envr ++a+E+l a+qgl+fr+ k++++le+++ ++R++v+ +e+DR++apD+e lcl|FitnessBrowser__Cup4G11:RR42_RS14640 419 SMAPNAGKRLWAMAENVRGILAVEWLGACQGLDFREGLKSSPKLEQARSLLRAEVPYYEKDRFFAPDIE 487 ********************************************************************* PP TIGR01225 484 avkellekesleaaveakvka 504 ++++ll++++l+a ++a++++ lcl|FitnessBrowser__Cup4G11:RR42_RS14640 488 TASRLLAAGTLNALLPARLLP 508 ************999987665 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (506 nodes) Target sequences: 1 (510 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.03 # Mc/sec: 7.11 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory