GapMind for catabolism of small carbon sources

 

Aligments for a candidate for permease in Cupriavidus basilensis 4G11

Align histidine permease (characterized)
to candidate RR42_RS28305 RR42_RS28305 proline-specific permease

Query= reanno::pseudo3_N2E3:AO353_12275
         (468 letters)



>lcl|FitnessBrowser__Cup4G11:RR42_RS28305 RR42_RS28305
           proline-specific permease
          Length = 472

 Score =  422 bits (1086), Expect = e-123
 Identities = 201/438 (45%), Positives = 294/438 (67%), Gaps = 1/438 (0%)

Query: 8   LKRGLSARHIRFMALGSAIGTGLFYGSASAIQMAGPAVLLAYLIGGAAVFMVMRALGEMA 67
           L RGL  RHI+ +A+G AIG GLF G+  AI +AGP ++L+Y IGG A+F +MRALGE+ 
Sbjct: 19  LHRGLKDRHIQMIAIGGAIGVGLFLGAGRAIAIAGPGLMLSYAIGGVAIFFIMRALGELL 78

Query: 68  VHNPVAGSFGQYASTYLGPMAGFILGWTYAFEMVIVGMADVTAFGIYMGFWFPEVSRWIW 127
           ++ PV+GSF  YA  ++GP AGF  GW+Y F  V+ GMA++TA  +Y+ +WFP+V +WI 
Sbjct: 79  LYRPVSGSFATYAEEFVGPFAGFATGWSYWFMWVVTGMAEITAVAVYVHYWFPDVPQWIP 138

Query: 128 VLGVVSIVGGLNLCNVKVFGEMEFWLSLLKVAAIVAMILGGFGIMLFGISTAPGQVTDIS 187
            L  ++++  +N   V VFGE+EFW +L+KV  IVAMI+ G  I+ FG+ T  G     S
Sbjct: 139 ALATLAVLYLVNCVAVAVFGELEFWFALIKVVTIVAMIVIGLAIIFFGV-TPLGPTASFS 197

Query: 188 NLWTQGGFMPNGMGGLIASFAVVMFAFGGIEIIGVTAGEAKDPQHVLPRAINAVPLRILL 247
           NLWT GGFMP G  G++ +  +VMFA+ G+E+IGVTAGEA++P+ VLP A N V  RIL+
Sbjct: 198 NLWTHGGFMPFGTLGVVLTLQIVMFAYQGVELIGVTAGEAQNPEKVLPHATNGVVWRILI 257

Query: 248 FYVLTMLVLMSIFPWQQIGSQGSPFVQIFDKLGISSAATILNIVVITAAISAINSDIFGA 307
           FYV  ++++M++ PW ++    SPFV +F+++G+  AA I+N+VVITAA S+ NS IF  
Sbjct: 258 FYVGALIIMMALVPWNELKPGVSPFVYVFERIGVPGAAAIVNLVVITAAASSCNSGIFST 317

Query: 308 GRMMFGLAQQGHAPKGFAHLSRNGVPWMTVVVMSVALLLGVLLNYLIPENVFLLIASIAT 367
           GRM++ LAQ G AP+ F  +S   VP + +   +  + +GVLLNY++PE VF+ + SI+ 
Sbjct: 318 GRMLYTLAQFGQAPRAFGRVSSKHVPSIAITFSAALMGIGVLLNYIVPEQVFVWVTSISL 377

Query: 368 FATVWVWLMILFTQVAMRRSMTAEQVAQLKFPVPFWPYAPMAAIAFMLFVFGVLGYFPDT 427
             ++W W +I+   +  R+++ A +V  + F +P  PYA    +AFM+ V  +L   P T
Sbjct: 378 VGSLWTWSIIMIAHLGYRKAIAAGRVKAVAFRMPGAPYANWLVVAFMIAVAVLLSLDPGT 437

Query: 428 QAALIVGVVWIVLLVLAY 445
           + AL V  VW  LL + Y
Sbjct: 438 RVALYVAPVWFALLGIGY 455


Lambda     K      H
   0.329    0.142    0.441 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 719
Number of extensions: 38
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 468
Length of database: 472
Length adjustment: 33
Effective length of query: 435
Effective length of database: 439
Effective search space:   190965
Effective search space used:   190965
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory