Align Peroxisomal bifunctional enzyme; PBE; PBFE; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 (characterized)
to candidate RR42_RS11095 RR42_RS11095 3-hydroxyacyl-CoA dehydrogenase
Query= SwissProt::Q08426 (723 letters) >FitnessBrowser__Cup4G11:RR42_RS11095 Length = 693 Score = 508 bits (1308), Expect = e-148 Identities = 301/711 (42%), Positives = 433/711 (60%), Gaps = 32/711 (4%) Query: 2 AEYTRLHNALALIRLRNPPVNAISTTLLRDIKEGLQKAVIDHTIKAIVICGAEGKFSAGA 61 A+Y ++ + +A+I L NPPVN + + I EG+ +A+ D +KAIVI GA FS GA Sbjct: 3 AQY-QVQDGVAVITLDNPPVNGLGLSTRLGIVEGMTRALDDAAVKAIVITGAGKAFSGGA 61 Query: 62 DIRGFSAPR-TFGLTLGHVVDEIQRNEKPVVAAIQGMAFGGGLELALGCHYRIAHAEAQV 120 DIR F+ P+ T TL V+ ++ + KPVVAAI +A GGGLELALGC+YR+A AQ+ Sbjct: 62 DIREFNTPKATQEPTLHSVIKVVEGSSKPVVAAIHSVAMGGGLELALGCNYRVASKGAQI 121 Query: 121 GLPEVTLGLLPGARGTQLLPRLTGVPAALDLITSGRRILADEALKLGILDKVVNSDPVEE 180 LPEV LGLLPGA GTQ LPR+ G+ AAL++I SG I +++ + D++V+ D + Sbjct: 122 ALPEVKLGLLPGAGGTQRLPRVIGLEAALNMIVSGNAIPSEKFAGTKLFDEIVDGDVLPA 181 Query: 181 AIRFAQRVSDQPLESRRLCNKPI--QSLPNMDSIFSEALLKMRRQHPGCLAQEACVRAVQ 238 A+ FA+ + P ++ + + ++ + + + + P L CV AV Sbjct: 182 AVAFAKTAAANPGPYPKVRDLKVKHENAEGFIAFSRNTVAAVAKNFPAPLK---CVDAVA 238 Query: 239 AAVQYPYEVGIKKEEELFLYLLQSGQARALQYAFFAERKANKW-STPSGASWKTASARPV 297 A+++ P+E G+K E E F+YL+ + ++RAL++AFF ER A+K P G + R + Sbjct: 239 ASLK-PFEAGLKAEREGFMYLITTPESRALRHAFFGERAASKIPDVPEGTA-----TRKI 292 Query: 298 SSVGVVGLGTMGRGIVISFARARIPVIAVDSDKNQLATANKMITSVLEKEASKMQQSGHP 357 V V+G GTMG GI ++F A +PVI +++ + L I E A K + + Sbjct: 293 ERVAVIGAGTMGGGITMNFLNAGVPVIMLETKQEALDRGVATIRKNYENSAKKGKLTQEK 352 Query: 358 WSGPKPRLTSSVK--ELGGVDLVIEAVFEEMSLKKQVFAELSAVCKPEAFLCTNTSALDV 415 LT+++ E+ D+VIEAVFEEM +K+ VF +L V K A L +NTS LDV Sbjct: 353 VEQRMGLLTTTLSYDEIKDADMVIEAVFEEMGVKEIVFKKLDEVMKQGAILASNTSTLDV 412 Query: 416 DEIASSTDRPHLVIGTHFFSPAHVMKLLEVIPSQYSSPTTIATVMNLSKKIKKIGVVVGN 475 ++IAS T RP V+G HFFSPA+VMKLLEV+ + + +ATVM L+KKIKK VV G Sbjct: 413 NKIASFTKRPQDVVGMHFFSPANVMKLLEVVRGEKTGKDVLATVMQLAKKIKKTAVVSGV 472 Query: 476 CFGFVGNRMLNPYYNQAYFLLEEGSKPEEVDQVLEEFGFKMGPFRVSDLAGLDVGWKSRK 535 C GF+GNRM+ Y QA +LL+EG+ PE+VD+ +E+FGF MGPFR+ DLAG D+GW RK Sbjct: 473 CDGFIGNRMIEQYSRQAGYLLDEGALPEQVDKAIEKFGFAMGPFRMGDLAGNDIGWAIRK 532 Query: 536 GQGLTGPTLLPGTPARKRGNRRYCPIPDVLCELGRFGQKTGKGWYQYDKPLGRIHKPDPW 595 + + P + +Y D+LCELGR+GQKTG GWY Y K R P+ Sbjct: 533 RRAIDKPEI------------QYSKTADLLCELGRYGQKTGAGWYDY-KAGDRKPYPNQQ 579 Query: 596 LSKFLSRYRKTHHIEPRTISQDEILERCLYSLINEAFRILGEGIAASPEHIDVVYLHGYG 655 ++ + ++ K I R IS +EI+ER +++L+NE +IL EGIA+ ID+VYL GYG Sbjct: 580 VNDMIVQHSKDLGITRRKISDEEIVERLVFALVNEGAKILEEGIASKASDIDMVYLTGYG 639 Query: 656 WPRHKGGPMFYASTVGLPTVLEKLQKYYR-QNPDIPQLEPSDYLKKLASQG 705 +P +GGPM YA VGL V + + +Y + + + Q P L KLA++G Sbjct: 640 FPLFRGGPMLYADQVGLFNVAQSMARYAKGYHGEAWQAAP--LLVKLAAEG 688 Lambda K H 0.319 0.136 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1130 Number of extensions: 55 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 1 Length of query: 723 Length of database: 693 Length adjustment: 39 Effective length of query: 684 Effective length of database: 654 Effective search space: 447336 Effective search space used: 447336 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory