Align NatE, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized)
to candidate RR42_RS20235 RR42_RS20235 metal-dependent hydrolase
Query= TCDB::Q8YT15 (247 letters) >FitnessBrowser__Cup4G11:RR42_RS20235 Length = 247 Score = 221 bits (562), Expect = 1e-62 Identities = 115/242 (47%), Positives = 164/242 (67%), Gaps = 8/242 (3%) Query: 11 LLEVENVHAGYIKDVDILQGVNFRVESGELVTVIGPNGAGKSTLAKTIFGLLT---PHTG 67 +LEV+++H Y K V+ + G N +VE+G++VTVIGPNGAGKST+ I G L TG Sbjct: 4 ILEVKDLHVRYGK-VEAVHGANLKVEAGKIVTVIGPNGAGKSTMLNAIMGALPVSGSSTG 62 Query: 68 KITFKGKNIAGLKSNQIVRLGMCYVPQIANVFPSLSVEENLEMGAFIRN----DSLQPLK 123 +T+ G ++AGL + V GMC VP+ +F S+SV +NL +GA+ R + Sbjct: 63 AVTYLGHDLAGLPAEGRVARGMCLVPEKRELFGSMSVADNLVLGAYRRKRAGEKNFLDQM 122 Query: 124 DKIFAMFPRLSDRRRQRAGTLSGGERQMLAMGKALMLEPSLLVLDEPSAALSPILVTQVF 183 + ++ +FPRL +R +Q AGTLSGGERQMLA+G+ALM +P LL+LDEPS L+P++V ++F Sbjct: 123 EVVYGLFPRLKERAKQDAGTLSGGERQMLAVGRALMAKPQLLMLDEPSLGLAPLIVKEIF 182 Query: 184 EQVKQINQEGTAIILVEQNARKALEMADRGYVLESGRDAISGPGQELLTDPKVAELYLGA 243 + + + G A +L+EQNAR AL++AD GYV+E+G A+ GP EL +PKV E YLG Sbjct: 183 HIINDLRKTGVATLLIEQNARAALQVADYGYVIETGDMAMEGPAGELAANPKVIETYLGL 242 Query: 244 GK 245 K Sbjct: 243 AK 244 Lambda K H 0.317 0.136 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 198 Number of extensions: 9 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 247 Length of database: 247 Length adjustment: 24 Effective length of query: 223 Effective length of database: 223 Effective search space: 49729 Effective search space used: 49729 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory