Align Methylcrotonoyl-CoA carboxylase (EC 6.4.1.4) (characterized)
to candidate RR42_RS32735 RR42_RS32735 3-methylcrotonyl-CoA carboxylase
Query= reanno::pseudo6_N2E2:Pf6N2E2_2194 (649 letters) >FitnessBrowser__Cup4G11:RR42_RS32735 Length = 674 Score = 541 bits (1395), Expect = e-158 Identities = 318/664 (47%), Positives = 409/664 (61%), Gaps = 33/664 (4%) Query: 8 TLLVANRGEIACRVMRTAKAMGLTTVAVHSATDRDARHSREADIRVDLGGSKAADSYLQI 67 TLLVANRGEIA R+MRTA+ +GL TVAV+S DR H AD V +G + SYL I Sbjct: 10 TLLVANRGEIAMRIMRTARRLGLATVAVYSEADRHGPHVAYADRAVRIGDAAPRASYLNI 69 Query: 68 DKLIAAAKASGAQAIHPGYGFLSENAGFARAIENAGLIFLGPPASAIDAMGSKSAAKALM 127 +I AA+ SGA A+HPGYGFL+ENA FA A+ AGL+F+GPPA+AI AMG+K+ AK LM Sbjct: 70 AAIIEAARRSGADAVHPGYGFLAENAEFADAVAQAGLVFVGPPAAAIRAMGNKAEAKRLM 129 Query: 128 ETAGVPLVPGYHGEAQDLETFRDAAERIGYPVLLKATAGGGGKGMKVVEDVSQLAEALAS 187 A +P +PGY GEAQD AA IG+P+++KA AGGGG+GM++V DV L ALAS Sbjct: 130 LAADMPCIPGYQGEAQDDAALLQAAAPIGFPLMVKAAAGGGGRGMRLVHDVHALPGALAS 189 Query: 188 AQREAQSSFGDSRMLVEKYLLKPRHVEIQVFADQHGNCLYLNERDCSIQRRHQKVVEEAP 247 A+ EA ++FG ++++E+ ++ PRHVEIQVFAD G+ ++L ERDCS+QRRHQKVVEEAP Sbjct: 190 ARSEAMAAFGSGQLILERAVIAPRHVEIQVFADTQGHVIHLGERDCSVQRRHQKVVEEAP 249 Query: 248 APGLTAQLRQAMGEAAVRAAQAIGYVGAGTVEFLLDARGEFFFMEMNTRLQVEHPVTEAI 307 +P ++ LR+ MG AAVRAAQAIGYVGAGT+EFLLD G F+FMEMNTRLQVEH VTEAI Sbjct: 250 SPAVSPALRERMGGAAVRAAQAIGYVGAGTMEFLLDGDGNFYFMEMNTRLQVEHAVTEAI 309 Query: 308 TGLDLVAWQIRVAQGEPLPITQAQVPLLGHAIEVRLYAEDPVNDFLPATGRLALYRESAK 367 TG DLV WQ+RVA GEPLP+TQ QV L GHAIEVRL AED FLP G + +R Sbjct: 310 TGFDLVEWQLRVAAGEPLPVTQEQVALRGHAIEVRLTAEDVAAGFLPQGGPVLRWRAPGA 369 Query: 368 GPGRRVDSGVEEGDEISPFYDPMLGKLIAWGENREQARLRLLSMLDEFAIGGLKTNIGFL 427 G RVD ++EG EI YD M+ KL+ G RE+AR +LL +++ + G+ +N GFL Sbjct: 370 GHDVRVDHALQEGGEIPVHYDSMVAKLVTHGATREEARRKLLRAVEQTVLLGVASNQGFL 429 Query: 428 RRIVAHPAFAAAELDTGFIPRYQAQLL----PEPGELDDAFWLAAAQGFALSLAPHIRGD 483 +AH AFA ++ TGF+ + L P ++ A LAAA A S P + Sbjct: 430 ADCLAHEAFAGNQVHTGFVDTHMQAALRTTQPAAAQVACAA-LAAAGLLAASECPALVAP 488 Query: 484 DAGSPWASTTGMRLGLSR-ETTLHLSCEGQDRAL-------------------TLDVTAH 523 A + G++ G + L +G + A+ + V Sbjct: 489 ------ACSLGLQAGEQQWRVDLRAVAQGWEVAVRGGEGEGEGEGEGDGTSAEIVSVRVL 542 Query: 524 CAELKGERLTIEHHGVRRSHRAIRQGDSLYLHWAGDLHRIDLYDPLAAAE--ASHSHQGG 581 A+L+ +E +G+ GD+++L AG R DP G Sbjct: 543 RADLEQGSALVECNGMALPLVFATGGDAVHLFSAGRAWRFARLDPRQKKRNPGDAGRAGE 602 Query: 582 LAAPMNGSIVRVLVSVGQPVDAGAQLVVLEAMKMEHSIRAPKAGVIKALYCQEGEMVSEG 641 L AP+ IV V + G+ V AG LVVLEAMKMEH+I AP GVI L+ +EG G Sbjct: 603 LLAPLTARIVAVKAAAGEAVLAGQVLVVLEAMKMEHAIVAPFDGVIAQLHAREGGQARAG 662 Query: 642 SALV 645 S LV Sbjct: 663 SLLV 666 Lambda K H 0.319 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1195 Number of extensions: 50 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 1 Length of query: 649 Length of database: 674 Length adjustment: 38 Effective length of query: 611 Effective length of database: 636 Effective search space: 388596 Effective search space used: 388596 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory