Align Butyrate--acetoacetate CoA-transferase subunit A; Short=Coat A; EC 2.8.3.9 (characterized, see rationale)
to candidate RR42_RS22970 RR42_RS22970 3-oxoadipate CoA-transferase
Query= uniprot:P33752 (218 letters) >FitnessBrowser__Cup4G11:RR42_RS22970 Length = 230 Score = 151 bits (381), Expect = 1e-41 Identities = 83/216 (38%), Positives = 124/216 (57%), Gaps = 2/216 (0%) Query: 1 MNSKIIRFENLRSFFKDGMTIMIGGFLNCGTPTKLIDFLVNLNIKNLTIISNDTCYPNTG 60 +N ++ + +DG T+M+GGF + G P LI ++ ++LT+ISN+ Y G Sbjct: 2 VNKRVASCAEAIADLRDGATVMVGGFGSAGLPDGLIAAVLAHGARDLTVISNNAGYDGEG 61 Query: 61 IGKLISNNQVKKLIASYIGSNPDTGKK--LFNNELEVELSPQGTLVERIRAGGSGLGGVL 118 + LI +++KL+ SY + T + E+E+EL QGTLVERIR G+GLGG Sbjct: 62 VASLIEAGRMRKLVCSYPLTAGATAFRDAYRRGEVELELVAQGTLVERIRCAGAGLGGFY 121 Query: 119 TKTGLGTLIEKGKKKISINGTEYLLELPLTADVALIKGSIVDEAGNTFYKGTTKNFNPYM 178 GT++ +GK ING ++LELPL AD ALI+G D GN Y +NFNP M Sbjct: 122 CPITAGTILAQGKDTREINGVLHVLELPLHADFALIRGRHADRLGNLDYHLAGRNFNPIM 181 Query: 179 AMAAKTVIVEAENLVSCEKLEKEKAMTPGVLINYIV 214 A AA+ V+ E E +V + E+ +TPG+ ++ +V Sbjct: 182 ATAARVVVAEVEAMVEAGAMNPEQIVTPGIFVHRVV 217 Lambda K H 0.315 0.136 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 131 Number of extensions: 7 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 218 Length of database: 230 Length adjustment: 22 Effective length of query: 196 Effective length of database: 208 Effective search space: 40768 Effective search space used: 40768 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory