Align ABC-type maltose transporter (EC 7.5.2.1) (characterized)
to candidate RR42_RS22875 RR42_RS22875 ABC transporter
Query= BRENDA::Q8NMV1 (376 letters) >FitnessBrowser__Cup4G11:RR42_RS22875 Length = 351 Score = 258 bits (658), Expect = 2e-73 Identities = 142/323 (43%), Positives = 194/323 (60%), Gaps = 35/323 (10%) Query: 1 MATVTFKDASLSYPGAKEPTVKKFNLEIADGEFLVLVGPSGCGKSTTLRMLAGLENVTDG 60 MATV + + + G V +L + + EFLVL+GPSG GK+T LR++AGLE T G Sbjct: 1 MATVETRSLTKRFDGTN--AVDGIDLAVHEAEFLVLLGPSGSGKTTLLRLIAGLEAPTSG 58 Query: 61 AIFIGDKDVTHVAPRDRDIAMVFQNYALYPHMTVGENMGFALKIAGKSQDEINKRVDEAA 120 I +G + VT + PR ++AMVFQ+YALYPH++V N+ F L+ ++ I ++V AA Sbjct: 59 DILVGGRVVTGLPPRAHNMAMVFQSYALYPHLSVAGNIAFPLEAQRMPREAIARKVSWAA 118 Query: 121 ATLGLTEFLERKPKALSGGQRQRVAMGRAIVRNPQVFLMDEPLSNLDAKLRVQTRTQIAA 180 A G+ L RKP+ LSGG+RQRVA+ RA+VR P FL+DEPLSNLDAKLR R ++ Sbjct: 119 ALFGIGHLLSRKPRQLSGGERQRVALARAVVREPVAFLLDEPLSNLDAKLRTSAREELQQ 178 Query: 181 LQRKLGVTTVYVTHDQTEALTMGDRIAVLKDGYLQQVGAPRELYDRPANVFVAGFIGSPA 240 LQR+L TT+YVTHDQ EAL +GDR+A+L G + Q+G P+++Y++PA+ FVA FIGSP Sbjct: 179 LQRRLATTTIYVTHDQIEALALGDRVAILDHGRVHQLGTPQQVYEQPADTFVATFIGSPP 238 Query: 241 MNLGTFSVKDGDATSGHARIKLSPETLAAMTPEDNGRITIGFRPEAL---EIIPEGESTD 297 MNL D + GFRPE E+ E+ + Sbjct: 239 MNL-----------------------------VDTDALVTGFRPEHFLPREVYGSDEALE 269 Query: 298 LSIPIKLDFVEELGSDSFLYGKL 320 P + +E LGSD +YG L Sbjct: 270 -PFPFHITRIENLGSDRLVYGLL 291 Lambda K H 0.316 0.135 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 340 Number of extensions: 15 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 376 Length of database: 351 Length adjustment: 29 Effective length of query: 347 Effective length of database: 322 Effective search space: 111734 Effective search space used: 111734 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory