Align phenylacetate-CoA ligase (EC 6.2.1.30) (characterized)
to candidate RR42_RS23755 RR42_RS23755 phenylacetate--CoA ligase
Query= BRENDA::B4EL89 (440 letters) >FitnessBrowser__Cup4G11:RR42_RS23755 Length = 441 Score = 726 bits (1873), Expect = 0.0 Identities = 352/437 (80%), Positives = 392/437 (89%) Query: 1 MTHPTHPAAALEPIETASRDELQALQLERLKWSLRHAYDNVPHYRRTFDAAGVHPDDLKS 60 M P LE IE ASRDELQALQL RLKWSLRHAYDNV HYRR FDAAGVHPDDL+ Sbjct: 1 MVQPIPQPGELEAIELASRDELQALQLARLKWSLRHAYDNVAHYRRAFDAAGVHPDDLRQ 60 Query: 61 LADLAKFPFSTKNDLRDNYPFGLFAVPREQVVRVHASSGTTGKPTVVGYTARDIDTWANV 120 L+DLAKFP +TK DLRDNYPFGLFAVP+++VVRVHASSGTTGKPTVVGYTA+DIDTWANV Sbjct: 61 LSDLAKFPTTTKKDLRDNYPFGLFAVPKDEVVRVHASSGTTGKPTVVGYTAKDIDTWANV 120 Query: 121 TARSIRAAGGRPGDTLHNAFGYGLFTGGLGIHYGAERLGCMVVPMSGGQTEKQVQLIRDF 180 TARSIRAAGGR GDTLHNAFGYGLFTGGLGIHYGAERLGCMVVPMSGGQTEKQVQLIRDF Sbjct: 121 TARSIRAAGGRRGDTLHNAFGYGLFTGGLGIHYGAERLGCMVVPMSGGQTEKQVQLIRDF 180 Query: 181 EPKIILVTPSYMLNLIDEMVRQGMDPAESSLKIGIFGAEPWTQALRNEVETRVGIDALDI 240 EP IILVTPSYMLNLIDEM RQGM+PAESSLKIGIFGAEPWTQ +R+E+ETR GI A+DI Sbjct: 181 EPSIILVTPSYMLNLIDEMARQGMNPAESSLKIGIFGAEPWTQGMRSEIETRAGIQAIDI 240 Query: 241 YGLSEVMGPGVACECVETKDGPVIWEDHFYPEIIDPVTGEVLPDGSQGELVFTSLTKEAM 300 YGLSE+MGPGVA EC+E+KDGPV+WEDHFYPEIIDPVTGEVLPDGSQGELVFTSL+KEAM Sbjct: 241 YGLSEIMGPGVASECIESKDGPVVWEDHFYPEIIDPVTGEVLPDGSQGELVFTSLSKEAM 300 Query: 301 PVIRYRTRDLTALLPPTARAMRRLAKITGRSDDMLIVRGVNVFPSQIEEIVVALPLLSGQ 360 P+IRYRTRDLTALL PT+R+MRRLAKITGRSDDMLI+RGVNVFPSQ+EE+++ + LSG Sbjct: 301 PMIRYRTRDLTALLAPTSRSMRRLAKITGRSDDMLIIRGVNVFPSQVEELILGIARLSGN 360 Query: 361 FQITLSRDGHMDRLDLAVELRSEAAASVTDGERAALARELQHRIKTMVGVSSGVTVLAAG 420 +Q+ ++RDGHMD L ++VE R+E S+ + ERA LARELQHR+KTM+GVS+ V VL AG Sbjct: 361 YQLCITRDGHMDSLSVSVEARAEICGSLAESERAQLARELQHRVKTMIGVSTAVRVLNAG 420 Query: 421 GIPATATGKARRVIDRR 437 I TATGKA+RV+D R Sbjct: 421 EIKTTATGKAQRVLDLR 437 Lambda K H 0.319 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 732 Number of extensions: 14 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 440 Length of database: 441 Length adjustment: 32 Effective length of query: 408 Effective length of database: 409 Effective search space: 166872 Effective search space used: 166872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 51 (24.3 bits)
Align candidate RR42_RS23755 RR42_RS23755 (phenylacetate--CoA ligase)
to HMM TIGR02155 (paaF: phenylacetate-CoA ligase (EC 6.2.1.30))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02155.hmm # target sequence database: /tmp/gapView.18066.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02155 [M=422] Accession: TIGR02155 Description: PA_CoA_ligase: phenylacetate-CoA ligase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.6e-223 727.7 0.0 1.9e-223 727.5 0.0 1.0 1 lcl|FitnessBrowser__Cup4G11:RR42_RS23755 RR42_RS23755 phenylacetate--CoA Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Cup4G11:RR42_RS23755 RR42_RS23755 phenylacetate--CoA ligase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 727.5 0.0 1.9e-223 1.9e-223 1 422 [] 15 437 .. 15 437 .. 0.99 Alignments for each domain: == domain 1 score: 727.5 bits; conditional E-value: 1.9e-223 TIGR02155 1 eklsldelralqlerlkvsvkrayenvpayrkafdaagvkpddlkelsdlakfpltaksdlrdnypfdl 69 e +s+del+alql+rlk+s+++ay+nv++yr+afdaagv+pddl++lsdlakfp t+k+dlrdnypf+l lcl|FitnessBrowser__Cup4G11:RR42_RS23755 15 ELASRDELQALQLARLKWSLRHAYDNVAHYRRAFDAAGVHPDDLRQLSDLAKFPTTTKKDLRDNYPFGL 83 6799***************************************************************** PP TIGR02155 70 lavprekvvrvhassGttGkptvvaytqkdldtwsevvarslraaGGrkgdllhnayGyGlftGGlGvh 138 +avp+++vvrvhassGttGkptvv+yt+kd+dtw++v ars+raaGGr+gd+lhna+GyGlftGGlG+h lcl|FitnessBrowser__Cup4G11:RR42_RS23755 84 FAVPKDEVVRVHASSGTTGKPTVVGYTAKDIDTWANVTARSIRAAGGRRGDTLHNAFGYGLFTGGLGIH 152 ********************************************************************* PP TIGR02155 139 yGaeklGatvvpisGGqtekqvqliqdfkpdiiavtpsyilalleelkrlgidpedislkvailGaepw 207 yGae+lG++vvp+sGGqtekqvqli+df+p+ii vtpsy+l+l++e+ r+g++p++ slk++i+Gaepw lcl|FitnessBrowser__Cup4G11:RR42_RS23755 153 YGAERLGCMVVPMSGGQTEKQVQLIRDFEPSIILVTPSYMLNLIDEMARQGMNPAESSLKIGIFGAEPW 221 ********************************************************************* PP TIGR02155 208 teamrkelearlgikaldiyGlseviGpGvanecvetkdGlviwedhfypeiidpetgevlpdGeeGel 276 t+ mr+e+e+r+gi+a+diyGlse++GpGva ec+e+kdG+v+wedhfypeiidp tgevlpdG++Gel lcl|FitnessBrowser__Cup4G11:RR42_RS23755 222 TQGMRSEIETRAGIQAIDIYGLSEIMGPGVASECIESKDGPVVWEDHFYPEIIDPVTGEVLPDGSQGEL 290 ********************************************************************* PP TIGR02155 277 vfttltkealpviryrtrdltrllpgtartmrrmdkikGrsddllilrGvnvfptqleevllkldklsp 345 vft+l+kea+p+iryrtrdlt ll +t+r+mrr++ki+Grsdd+li+rGvnvfp+q+ee++l++ +ls+ lcl|FitnessBrowser__Cup4G11:RR42_RS23755 291 VFTSLSKEAMPMIRYRTRDLTALLAPTSRSMRRLAKITGRSDDMLIIRGVNVFPSQVEELILGIARLSG 359 ********************************************************************* PP TIGR02155 346 hyqleltreGaldeltlkvelkdesaalrlleqksllakkiekkikaevgvsvdvelvepgsler.seG 413 +yql +tr+G++d+l++ ve++ e + +++++++la+++++++k+++gvs+ v++ ++g ++ +G lcl|FitnessBrowser__Cup4G11:RR42_RS23755 360 NYQLCITRDGHMDSLSVSVEARAEICGSLAESERAQLARELQHRVKTMIGVSTAVRVLNAGEIKTtATG 428 ****************************************************************9899* PP TIGR02155 414 kakrvvdkr 422 ka+rv+d+r lcl|FitnessBrowser__Cup4G11:RR42_RS23755 429 KAQRVLDLR 437 *******98 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (422 nodes) Target sequences: 1 (441 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 9.52 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory