Alignments for a candidate for paaZ2 in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Alignments for a candidate for paaZ2 in Cupriavidus basilensis 4G11

Align oxepin-CoA hydrolase (EC 3.3.2.12) (characterized)
to candidate RR42_RS35145 RR42_RS35145 aldehyde dehydrogenase

Query= BRENDA::P77455
         (681 letters)



>FitnessBrowser__Cup4G11:RR42_RS35145
          Length = 514

 Score =  417 bits (1072), Expect = e-121
 Identities = 233/511 (45%), Positives = 316/511 (61%), Gaps = 6/511 (1%)

Query: 4   LASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAARQFAIEKGAPALRAMTFIE 63
           L+++L G WQ+G G    +   + G+AL  V + GLD+AA   FA E+G  ALRAMT+ E
Sbjct: 5   LSNYLGGRWQAGSGAGATLSDPVLGDALVRVDATGLDLAAGFAFAREQGGAALRAMTYRE 64

Query: 64  RAAMLKAVAKHLLSEKERFYALS-AQTGATRADSWVDIEGGIGTLFTYASLGSRELPDDT 122
           RAAML A+ K L + ++ +Y ++ A +G    DS VDI+GGI TL TYA LG   L +  
Sbjct: 65  RAAMLAAIVKILQTNRDAYYEIATANSGTVHNDSAVDIDGGIFTLGTYAKLGDA-LGERR 123

Query: 123 LWPEDELIPLSKEGGFAARHLLTSKSGVAVHINAFNFPCWGMLEKLAPTWLGGMPAIIKP 182
              + +   L K+  F ++H+L    GVA+ INAFNFP WG+ EK AP  L G+P I+KP
Sbjct: 124 YLIDGDAARLGKDPLFQSQHVLVPTRGVALLINAFNFPSWGLWEKAAPALLAGVPVIVKP 183

Query: 183 ATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDLLDHLDSQDVVTFTGSAATGQMLRV 242
           ATATA LTQ MV+ +VD+G++P GA+S++CGSA  LLD L   DVV+FTGSA T  ++R 
Sbjct: 184 ATATAWLTQRMVRDVVDAGVLPPGALSVVCGSAAGLLDQLQPFDVVSFTGSAQTAALIRS 243

Query: 243 QPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEFALFIREVVREMTTKAGQKCTAIRRII 302
              +  +S+   +EADS+N  +L          F L  +E  REMT K+GQKCTAIRRI 
Sbjct: 244 HAAVTQRSVRVNIEADSVNSALLLPGEAAGSEAFDLLAKEAAREMTVKSGQKCTAIRRIF 303

Query: 303 VPQALVNAVSDALVARLQKVVVGDPAQEGVKMGALVNAEQRADVQEKVNILLAAGCEIRL 362
           VP+AL  A +DA+ ARL +V VG+P  E V+MGALV+  Q A V+E +  L A    +  
Sbjct: 304 VPEALYGAAADAIGARLARVTVGNPRHEAVRMGALVSRAQLASVREGLGYLQAQAEVLHD 363

Query: 363 GGQADL----SAAGAFFPPTLLYCPQPDETPAVHATEAFGPVATLMPAQNQRHALQLACA 418
           G    L     A      PTLL          VH TE FGPVATL+P ++   AL L   
Sbjct: 364 GATHALVDADPAVACCVGPTLLGARDAHAADRVHDTEVFGPVATLVPYRDNADALALVRR 423

Query: 419 GGGSLAGTLVTADPQIARQFIADAARTHGRIQILNEESAKESTGHGSPLPQLVHGGPGRA 478
           G GSL  +L  +D     +   + A +HGR+ +++ + A+  TGHG+ +PQ +HGGPGRA
Sbjct: 424 GQGSLVASLYGSDADALAKAAVELADSHGRVHVISPDVAQLHTGHGNVMPQSLHGGPGRA 483

Query: 479 GGGEELGGLRAVKHYMQRTAVQGSPTMLAAI 509
           GGGEELGGLRA+  Y +R+AVQ S ++LA +
Sbjct: 484 GGGEELGGLRALNFYHRRSAVQASTSVLAQL 514


Lambda     K      H
   0.319    0.134    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 814
Number of extensions: 28
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 681
Length of database: 514
Length adjustment: 37
Effective length of query: 644
Effective length of database: 477
Effective search space:   307188
Effective search space used:   307188
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

Align oxepin-CoA hydrolase (EC 3.3.2.12) (characterized)
to candidate RR42_RS24800 RR42_RS24800 dehydratase

Query= BRENDA::P77455
         (681 letters)



>FitnessBrowser__Cup4G11:RR42_RS24800
          Length = 171

 Score = 71.6 bits (174), Expect = 3e-17
 Identities = 44/129 (34%), Positives = 74/129 (57%), Gaps = 8/129 (6%)

Query: 531 FEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAHMDKIAAAESIFGERVVHGYFVLSA 590
           F+ELQ G    +  RT+TEAD+VN+  L+G+    H +   A+++ FG+R+V G    S 
Sbjct: 17  FDELQIGQVFRSGGRTVTEADVVNYCALTGNWIEIHSNTHYASKTRFGQRLVQGSLTYSI 76

Query: 591 AAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTIQVRLTCKRKTLKKQRSAEEKPTGVVE 650
             GL +  G+G + ANYG++++RF  PV   DTI     C+    K+    +++  GV++
Sbjct: 77  VTGL-IQFGLG-IQANYGIDNMRFRNPVYINDTIYA--VCEVIGKKE----KDEKYGVIK 128

Query: 651 WAVEVFNQH 659
           + +   NQ+
Sbjct: 129 FRISAINQN 137


Lambda     K      H
   0.319    0.134    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 218
Number of extensions: 14
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 681
Length of database: 171
Length adjustment: 28
Effective length of query: 653
Effective length of database: 143
Effective search space:    93379
Effective search space used:    93379
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources