Align 6-carboxyhex-2-enoyl-CoA hydratase (characterized)
to candidate RR42_RS10265 RR42_RS10265 3-hydroxyacyl-CoA dehydrogenase
Query= metacyc::MONOMER-20678 (699 letters) >FitnessBrowser__Cup4G11:RR42_RS10265 Length = 692 Score = 640 bits (1652), Expect = 0.0 Identities = 337/685 (49%), Positives = 465/685 (67%), Gaps = 14/685 (2%) Query: 13 VAIVTVDSPPVNALSAAVRRGILENVNAAVADPAVQAIVLVCAGRTFIAGADITEFGKPP 72 VA++++ +PPVN L VR GI++ ++ A AD V+AIVL AG+ F GAD+ EF P Sbjct: 11 VAVISIANPPVNGLGYDVRVGIIDGIDRAAADDNVRAIVLTGAGKAFSGGADMREFNSPE 70 Query: 73 --QPPALNDVIAALENSPKPTIAAIHGTALGGGLEVALGCHFRVAVKEAKLGLPEVKLGL 130 + P LN V+ A+ENS K +AA+H A+GGGLE+AL CH+RVAV +A++ L EV +GL Sbjct: 71 AWREPGLNTVLVAIENSIKLVVAAVHSVAMGGGLELALACHYRVAVPKARIALSEVTMGL 130 Query: 131 LPGAGGTQRLPRAVGPELAVQMIVGGSPIGAAEALKHGLVEEVVE-NLVAGAVAFAKKVL 189 LPGAGGTQRLPR +G E A MIV G+ I + GL + +VE ++V AVAFA++V Sbjct: 131 LPGAGGTQRLPRLIGLEAATNMIVHGTSIPSEALADTGLFDRLVEGDVVTAAVAFAQEVA 190 Query: 190 AEKRPLRRLRDDDSKLAAAKADRSIFTNAVAAMTKKARGLEAPFACADAIGAAIDLPFEE 249 A P R+RD + + ++ AVAA K L+AP C DAI A+ LPF+E Sbjct: 191 ARPGPHPRVRDLTIRHPTPEGYLAVARPAVAARYKN---LQAPLRCLDAIEASFKLPFDE 247 Query: 250 GLKKEREGFMKLVVSDQSKAQRYAFFAEREAAKVDGVPDGTKPRPVSRVAIIGAGTMGGG 309 GL ER F++L+ SK+ R+AFF+ER AAK+ VPD R V++VA++GAG MG G Sbjct: 248 GLALERRLFLELMNGPVSKSLRHAFFSERAAAKLADVPDSVPERAVNKVAVVGAGLMGSG 307 Query: 310 IAMSFANAGIPVTLIETGEEQLKRGLGIMQKNWEATAARGGLPPDAPAKRMALITGLVGL 369 IAM+F +AGIPV L++ +E +++G +++N+EA+ +G L + RMAL+ V L Sbjct: 308 IAMNFLSAGIPVVLLDISDEVVQKGAAAIRRNYEASLKKGKLRAEVVEARMALLQPTVDL 367 Query: 370 ENVKDADLIIEAVFETMAVKKEVFTAVDAHAKPGAVLASNTSYLSIDEIAATTKRPQDVL 429 V DL+IEA++E M VK +F A+D A+PGA+LA+NTS L +D IAA T+RPQDV+ Sbjct: 368 GRVAACDLVIEAIYEDMDVKLRMFRALDEIARPGAILATNTSMLDVDRIAAETRRPQDVV 427 Query: 430 GMHFFSPANVMKLCEIVRGAKTAPDALLTAVSIAKKIAKVPVVVGVCDGFVGNRMLAARS 489 G+HFFSPANVM+L E+VRGAKTAPD L TA+ +A++I KVPVV GVCDGF+GNRMLA + Sbjct: 428 GLHFFSPANVMRLLEVVRGAKTAPDVLQTAMRLARRIGKVPVVSGVCDGFIGNRMLAKYT 487 Query: 490 KQSEKLLFEGALPQQVDAVVTKFGMPMGPFAMGDLAGLDIGWRSRKDRGIKS------EI 543 +++ +LL +GA P+QVD + +FG+ MGPF M DLAG DIGW RK + E+ Sbjct: 488 RRAGELLEQGAFPEQVDRAIEEFGLAMGPFRMADLAGNDIGWAIRKRHYAEDPSQPRFEL 547 Query: 544 ADALCEAGRFGQKTGKGYYKYEQGSRAPMPDPEVETLINDTLAKLGLKRRDITDEEILER 603 D LCEAG FGQKTG G+Y YE G R +P ++ + G+ R + +EI+ER Sbjct: 548 GDRLCEAGHFGQKTGTGWYDYEAGQRKALPSARTRAMLEAYWKERGVVPRRFSGQEIVER 607 Query: 604 MVYPMINEGARILEEKIAARPSDIDVVWLYGYGWPIYRGGPMHYADSVGLKHIAERLSAY 663 ++Y ++NEGA +L+E IAAR SDID V+L+GYG+P +RGGPM YAD+VG+ ++ L A Sbjct: 608 LIYALVNEGAAVLDEGIAARASDIDAVYLHGYGFPAWRGGPMFYADTVGVTNVRRTLRAL 667 Query: 664 AKATNDPSLEPAPLLARLAAEGKTF 688 + DPS +PA L+ +LA +G+TF Sbjct: 668 --SAKDPSWKPAALIEQLADKGRTF 690 Lambda K H 0.317 0.134 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1119 Number of extensions: 61 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 699 Length of database: 692 Length adjustment: 39 Effective length of query: 660 Effective length of database: 653 Effective search space: 430980 Effective search space used: 430980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory