GapMind for catabolism of small carbon sources

 

Alignments for a candidate for livF in Cupriavidus basilensis 4G11

Align High-affinity branched-chain amino acid transport ATP-binding protein (characterized, see rationale)
to candidate RR42_RS00210 RR42_RS00210 ABC transporter ATP-binding protein

Query= uniprot:A0A159ZWL6
         (233 letters)



>FitnessBrowser__Cup4G11:RR42_RS00210
          Length = 241

 Score =  230 bits (587), Expect = 2e-65
 Identities = 113/232 (48%), Positives = 163/232 (70%), Gaps = 1/232 (0%)

Query: 2   LQFENVSTFYGKIQALHSVNVEVRQGEIVTLIGANGAGKSTLLMTLCGSPQAHSGSIRYM 61
           L  + ++T Y K+  LH V+++V  G+I  ++GANGAGKSTL+  + G      G + + 
Sbjct: 10  LSVKGLTTGYDKVNVLHDVSIDVAPGKITCILGANGAGKSTLIRAILGLTPPRQGQVLWD 69

Query: 62  GEELVGQDSSHIMRKSIAVVPEGRRVFARLTVEENLAMGGFF-TDKGDYQEQMDKVLHLF 120
           G++L G+ + +I+   IA +PEGR++F R+TV ENLA+G +  TD    ++++ KV  +F
Sbjct: 70  GKDLAGEKTHNIIATGIACIPEGRKIFPRMTVAENLALGAYLETDAARVRDRLAKVYDIF 129

Query: 121 PRLKERFTQRGGTMSGGEQQMLAIGRALMSKPKLLLLDEPSLGLAPIIIQQIFDIIEQLR 180
           PRLKER TQ  GTMSGGEQ M++IGR LM++PKLL++DEPSLGL+P+ +++ F +I+Q+ 
Sbjct: 130 PRLKERATQLAGTMSGGEQAMVSIGRGLMAEPKLLVIDEPSLGLSPLYVKENFKVIKQIN 189

Query: 181 KDGVTVFLVEQNANQALKIADRAYVLENGRVVMQGTGEALLTDPKVREAYLG 232
             G+TV LVEQNA Q L I+   YVL  GRVV QGT EAL ++ +VR AY G
Sbjct: 190 ALGITVLLVEQNARQTLAISHYGYVLSQGRVVAQGTAEALASNDEVRSAYFG 241


Lambda     K      H
   0.320    0.137    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 166
Number of extensions: 3
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 233
Length of database: 241
Length adjustment: 23
Effective length of query: 210
Effective length of database: 218
Effective search space:    45780
Effective search space used:    45780
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory