Alignments for a candidate for pccB in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Alignments for a candidate for pccB in Cupriavidus basilensis 4G11

Align Propionyl-CoA carboxylase beta chain; PCCase; Propanoyl-CoA:carbon dioxide ligase; EC 6.4.1.3 (characterized)
to candidate RR42_RS18165 RR42_RS18165 carbamoyl-phosphate synthase large subunit

Query= SwissProt::P53003
         (546 letters)



>FitnessBrowser__Cup4G11:RR42_RS18165
          Length = 1112

 Score =  203 bits (516), Expect = 3e-56
 Identities = 162/551 (29%), Positives = 246/551 (44%), Gaps = 42/551 (7%)

Query: 9    GVPPAEAPDIHTTAGKLADLYRRNHEAVHAGSERAVAKQHAKGKRTARERIDMLLDEGSF 68
            G     A D       L D   R+     A   +AV K HA+G RTARE I  L D GSF
Sbjct: 579  GAQAGAAVDPAHIRADLRDALARHALTQDAARPQAVDKHHARGGRTARENIAQLCDAGSF 638

Query: 69   VELDEHA----RHRSTNFGMDADRPYGDGVVTGWGTVDGRRV-------CVFSQDFTVFG 117
            +E    A    R R +   ++   P  DG+VTG GTV+  R         V + D+TV  
Sbjct: 639  IEYGALAIAAQRQRRSVEDLERATP-ADGIVTGIGTVNAERFPATDARCMVLAYDYTVLA 697

Query: 118  GSLGEVFGEKIVKVMDLAMKTGCPLVGINDSGGARI--QEGVAALGLYAEIFKRNTHASG 175
            G+ G    +K+ +++ LA +   P+V   + GG R    +     GL    F +    SG
Sbjct: 698  GTQGFYGHKKLDRMLALARQWRLPVVLFAEGGGGRPGDTDTPVVAGLDCTSFIQFARLSG 757

Query: 176  VIPQISLIMGPCAGGAVYSPAITDFTVMVDQTSHMFITGPDVIKTVTGEDVSFEDLGGAR 235
             +P + ++ G C  G        D  ++  + + + + GP +I+       + E++G   
Sbjct: 758  QVPLVGIVSGRCFAGNAALLGCAD-VIIATRNATIGMGGPAMIEGGGLGVFAAEEVGPVS 816

Query: 236  THNERSGNAHYLATDEDDAISYVKELLSFLPSNNLSSSPVFPGAEVEEGSVADGVGDADL 295
                 +G    L  DE  A+   +  LS+                  +G + D       
Sbjct: 817  VQAP-NGVIDVLVDDEIAAVETARRYLSYF-----------------QGDLPDWEAGDAR 858

Query: 296  ELDALVPDSPNQPYDMREVITRLVDEGEFLEVSALFAPNMLCGFGRIEGASVGVVANQPM 355
             L  L+P++  + YDMR V+  L D    LE+ A F   ++    RIEG + G +AN P 
Sbjct: 859  ALRHLIPENRLRSYDMRGVLAALADHDSVLELRAAFGAGIITALIRIEGRAFGCIANNPR 918

Query: 356  QLAGTLDIDASEKAARFVRFCDAFNIPVLTLVDVPGFLPGTGQEWNGIIRRGAKLLYAYA 415
             L G +D DA++KAARF++ CDA  +P+++L D PGF+ G   E    +R  ++L     
Sbjct: 919  HLGGAIDADAADKAARFMQLCDAHGLPIVSLCDTPGFMVGPEAEKTATVRHVSRLFVTAG 978

Query: 416  EATVPLVTVITRKAYG-GAYDVMGSKHLGADINLAWPTAQIAVMGAQGAANILYRRQLAE 474
               VP  TV+ RK YG GA  + G          AWP+ +   MG +G+  + +R++L E
Sbjct: 979  ALRVPFFTVVLRKGYGLGAQAMAGGSFSAPFFTAAWPSGEFGAMGIEGSIRLGFRKEL-E 1037

Query: 475  AAERGEDVEALRARLQQEYEDTLCNPYVAAERGYVDSVIPPSHTRGHVARALRMLADKRE 534
            A    ++ EAL AR+  E           A    +D+VI P+ TR  + R L        
Sbjct: 1038 AVADPDEREALFARM-IEGAYRRGRALNMASHLEIDAVIDPADTRHWLLRGL------AS 1090

Query: 535  ALPAKKHGNIP 545
            ALPA    + P
Sbjct: 1091 ALPASAARDTP 1101


Lambda     K      H
   0.318    0.136    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1424
Number of extensions: 71
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 546
Length of database: 1112
Length adjustment: 41
Effective length of query: 505
Effective length of database: 1071
Effective search space:   540855
Effective search space used:   540855
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources