Alignments for a candidate for prpE in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Alignments for a candidate for prpE in Cupriavidus basilensis 4G11

Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate RR42_RS10650 RR42_RS10650 acetyl-CoA synthetase

Query= SwissProt::P27550
         (652 letters)



>FitnessBrowser__Cup4G11:RR42_RS10650
          Length = 588

 Score =  326 bits (835), Expect = 2e-93
 Identities = 206/565 (36%), Positives = 294/565 (52%), Gaps = 30/565 (5%)

Query: 73  GTLNLAANCLDRHLQENGDRTAIIWEGDDASQSKHISYKELHRDVCRFANTLLELGIKKG 132
           G LN+A   +DRH   +    A        + +  ++Y +L     RF N L +LG+ KG
Sbjct: 45  GALNIAWQAVDRHAASSLRGKAAFRILARGTPTHFVTYGQLSALTNRFCNVLRDLGVGKG 104

Query: 133 DVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPEAVAGRIIDSNSRLVITSDEGVRA 192
           + + I    +PE  +A+L   + G   S +F  F P+ +A R+      +++T+D     
Sbjct: 105 ERLFILAGRIPELYIAILGSLKNGTAVSPLFSAFGPDPIATRVNLGEGAVLVTTD----- 159

Query: 193 GRSIPLKKNVDDALKNPNVTSVEHVVVLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEE 252
              +  ++ +  A     + S++HV+++   GG        DL    L+  ASD  +   
Sbjct: 160 ---MLFERKI--AKWRDRMPSLKHVLLVAEDGGTTAVPGTLDL--SSLMASASDACEITP 212

Query: 253 MNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTG 312
             AED   + +TSG+TG PKG +H  G  L + + T +Y  D H  DIYWCTAD GWVTG
Sbjct: 213 TTAEDMALLHFTSGTTGTPKGAVHVHGAVLTHWS-TGRYALDLHVDDIYWCTADPGWVTG 271

Query: 313 HSYLLYGPLACGATTLMFEGVPNWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAI 372
            SY +  PL  G T+++     +     R   ++    V+I YTAPTAIR LM  G    
Sbjct: 272 TSYGVIAPLLHGVTSIVDREEFD---AERWYAILQDEGVSIWYTAPTAIRMLMKAGADIA 328

Query: 373 EGTDRSSLRILGSVGEPINPEAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATEL 432
           +      LR   SVGEP+NPEA  W  + +G    P  D WWQTETGG MI   P A ++
Sbjct: 329 KKYAFPRLRFAASVGEPLNPEAVWWGKQVLG---LPFHDNWWQTETGGIMIANTP-AFDI 384

Query: 433 KAGSATRPFFGVQPALVDNEGNP----LEGAT-EGSLVITDSWPGQARTLFGDHERFEQT 487
           K GS  RP  GV+ A+V  + +     +E    EG L +   WP   R      ER+ ++
Sbjct: 385 KPGSMGRPLPGVEAAIVSRQADGSVQVIEAPNKEGELALKRGWPSMFRGYLNSEERYRKS 444

Query: 488 YFSTFKNMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAV 547
           +   +   Y +GD ARRD DGYYW  GR DDV+  +GH +G  E+ESAL+ HP +AEAAV
Sbjct: 445 FSGEW---YLTGDLARRDADGYYWFVGRADDVIKSAGHLIGPFEVESALMEHPAVAEAAV 501

Query: 548 VGIPHNIKGQAIYAYVTLNHGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRS 607
           +G P  I G+ + A+V+LN+G E S  L   +    R  +G    P  + +   LP+TRS
Sbjct: 502 IGKPDPIAGEVVKAFVSLNNGFEQSEALRMALLGHARTRLGAAVAPKEIAFLTQLPRTRS 561

Query: 608 GKIMRRILRKIAAGDTSNLGDTSTL 632
           GKIMRR+L+    G     GDTSTL
Sbjct: 562 GKIMRRLLKARELGLPE--GDTSTL 584


Lambda     K      H
   0.317    0.135    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1094
Number of extensions: 73
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 652
Length of database: 588
Length adjustment: 37
Effective length of query: 615
Effective length of database: 551
Effective search space:   338865
Effective search space used:   338865
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources