Aligments for a candidate for gabD in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Aligments for a candidate for gabD in Cupriavidus basilensis 4G11

Align NAD(P)-dependent succinate-semialdehyde dehydrogenase (EC 1.2.1.16) (characterized)
to candidate RR42_RS21375 RR42_RS21375 succinate-semialdehyde dehydrogenase

Query= metacyc::MONOMER-15736
         (480 letters)



>FitnessBrowser__Cup4G11:RR42_RS21375
          Length = 488

 Score =  684 bits (1766), Expect = 0.0
 Identities = 342/484 (70%), Positives = 393/484 (81%), Gaps = 5/484 (1%)

Query: 1   MQLKDAQLFRQQAFIDGAWVDADNGQTIKVNNPATGEILGTVPKMGAAETRRAIEAADKA 60
           MQLKD  L R QAFI G W  AD+G T  V NPA G ++GTVP MGAAET RAIEAA  A
Sbjct: 1   MQLKDPTLLRSQAFIGGQWQSADSGATFPVTNPADGSLIGTVPLMGAAETTRAIEAARVA 60

Query: 61  LPAWRALTAKERATKLRRWYELLIENQDDLGRLMTLEQGKPLAEAKGEIAYAASFIEWFA 120
             AWR  TA+ERA  LR WY+L++ N DDL  LMT EQGKPLAEA+GE  YAASF+EWFA
Sbjct: 61  QAAWRRKTARERAQVLRAWYDLMLANADDLAVLMTTEQGKPLAEARGEAVYAASFLEWFA 120

Query: 121 EEAKRIYGDVIPGHQPDKRLIVIKQPIGVTAAITPWNFPAAMITRKAGPALAAGCTMVIK 180
           E+AKR++GDV+     DKRL+V+K+P+GV AAITPWNFP AMITRKAGPALAAGC MV+K
Sbjct: 121 EQAKRVHGDVLATPASDKRLLVVKEPVGVCAAITPWNFPLAMITRKAGPALAAGCAMVLK 180

Query: 181 PASQTPFSALALVELAHRAGIPKGVLSVVTGSAG---DIGGELTSNPIVRKLSFTGSTEI 237
           PA  TP SALAL  LA RAG+P G+LSVVTG A    +IG ELT +P+VRKLSFTGSTE+
Sbjct: 181 PAEDTPLSALALALLAERAGLPAGLLSVVTGDAASSIEIGAELTGSPVVRKLSFTGSTEV 240

Query: 238 GRQLMAECAKDIKKVSLELGGNAPFIVFDDADLDKAVEGAIISKYRNNGQTCVCANRLYI 297
           GR LM + A  IKK+SLELGGNAPFIVFDDADLD AVEGA+ SKYRN GQTCVCANRLY+
Sbjct: 241 GRILMRQSAPTIKKLSLELGGNAPFIVFDDADLDAAVEGAMASKYRNAGQTCVCANRLYV 300

Query: 298 QDSVYDAFAEKLKAAVAKLKIGNGLEEGTTTGPLIDEKAVAKVQEHIADALKKGATLLAG 357
            D VYDAFA+KL AAV  LK+G+GLE G   GPLI+E AVAKV++HIADAL KGA LL G
Sbjct: 301 HDKVYDAFAQKLVAAVKTLKVGHGLEPGVQQGPLINEDAVAKVEQHIADALGKGARLLTG 360

Query: 358 GK--SMEGNFFEPTILVNVPKDAAVAKEETFGPLAPLFRFKDEAEVIAMSNDTEFGLASY 415
           GK   + G FFEPT+L NV  D  VAK+ETFGPLAPLFRF  + EV+ M+NDTEFGLASY
Sbjct: 361 GKRHDLGGTFFEPTVLANVTPDMVVAKQETFGPLAPLFRFTSDEEVVNMANDTEFGLASY 420

Query: 416 FYARDLGRVFRVAEALEYGMVGVNTGLISNEVAPFGGIKASGLGREGSKYGIEDYLEIKY 475
           F++RD+GR++RVAEALEYGMVG+NTGLISNEVAPFGG+K SGLGREG+ YGIE+YLE+KY
Sbjct: 421 FFSRDIGRIWRVAEALEYGMVGINTGLISNEVAPFGGVKQSGLGREGASYGIEEYLEVKY 480

Query: 476 LCLG 479
           LC+G
Sbjct: 481 LCMG 484


Lambda     K      H
   0.317    0.135    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 726
Number of extensions: 16
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 480
Length of database: 488
Length adjustment: 34
Effective length of query: 446
Effective length of database: 454
Effective search space:   202484
Effective search space used:   202484
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources