Align NAD(P)-dependent succinate-semialdehyde dehydrogenase (EC 1.2.1.16) (characterized)
to candidate RR42_RS21760 RR42_RS21760 succinate-semialdehyde dehydrogenase
Query= metacyc::MONOMER-15736 (480 letters) >FitnessBrowser__Cup4G11:RR42_RS21760 Length = 482 Score = 702 bits (1813), Expect = 0.0 Identities = 349/480 (72%), Positives = 404/480 (84%), Gaps = 4/480 (0%) Query: 1 MQLKDAQLFRQQAFIDGAWVDADNGQTIKVNNPATGEILGTVPKMGAAETRRAIEAADKA 60 +QL+D L RQQ +IDG W DA + I V NPATGE +G VP +GA ETR+AIEAA++A Sbjct: 2 LQLQDPSLLRQQCYIDGRWTDAQ--RHIDVTNPATGERVGQVPLLGADETRQAIEAANRA 59 Query: 61 LPAWRALTAKERATKLRRWYELLIENQDDLGRLMTLEQGKPLAEAKGEIAYAASFIEWFA 120 LPAWRA TAKER+ LR+W+ELL+ NQDDL R+MT EQGKP AEA+GEI YAASFIEWFA Sbjct: 60 LPAWRARTAKERSALLRKWFELLLANQDDLARIMTAEQGKPFAEARGEIGYAASFIEWFA 119 Query: 121 EEAKRIYGDVIPGHQPDKRLIVIKQPIGVTAAITPWNFPAAMITRKAGPALAAGCTMVIK 180 EE KR+YG+ IP ++R++V K+P+GV AAITPWNFPAAMITRKAGPALAAGCTMV+K Sbjct: 120 EEGKRVYGETIPAPVSNQRIVVTKEPVGVCAAITPWNFPAAMITRKAGPALAAGCTMVVK 179 Query: 181 PASQTPFSALALVELAHRAGIPKGVLSVVTGSAGDIGGELTSNPIVRKLSFTGSTEIGRQ 240 PASQTP +ALA+V LA RAGIP GVLSVVTGSA IGGEL+SNP+VRKL+FTGSTE+GR Sbjct: 180 PASQTPLTALAMVALAERAGIPAGVLSVVTGSAAAIGGELSSNPLVRKLTFTGSTEVGRT 239 Query: 241 LMAECAKDIKKVSLELGGNAPFIVFDDADLDKAVEGAIISKYRNNGQTCVCANRLYIQDS 300 LMA+ A IKKVS+ELGGNAPFIVF+DADLD AVEGAI+SKYRN GQTCVCANRLY+ Sbjct: 240 LMAQTASTIKKVSMELGGNAPFIVFEDADLDAAVEGAIVSKYRNAGQTCVCANRLYVHSK 299 Query: 301 VYDAFAEKLKAAVAKLKIGNGLEEGTTTGPLIDEKAVAKVQEHIADALKKGATLLAGGK- 359 VYDAFAEKL AAV LK+GNG+E+G GPLID KAV KV+EHI DA+ KGA +L GGK Sbjct: 300 VYDAFAEKLVAAVRALKVGNGMEDGVRIGPLIDGKAVTKVEEHITDAISKGARVLQGGKR 359 Query: 360 -SMEGNFFEPTILVNVPKDAAVAKEETFGPLAPLFRFKDEAEVIAMSNDTEFGLASYFYA 418 ++ +FFEPT+L +V VA+EETFGPLAPLFRF+ E EV+AM+NDTEFGLASYFYA Sbjct: 360 HALGQSFFEPTVLADVTPGMLVAREETFGPLAPLFRFETEDEVVAMANDTEFGLASYFYA 419 Query: 419 RDLGRVFRVAEALEYGMVGVNTGLISNEVAPFGGIKASGLGREGSKYGIEDYLEIKYLCL 478 RDLGRV+RV+E LEYGMVGVNTGLISNEVAPFGG+K SG+GREGS YGIEDYL IKY C+ Sbjct: 420 RDLGRVWRVSERLEYGMVGVNTGLISNEVAPFGGVKQSGVGREGSHYGIEDYLVIKYTCM 479 Lambda K H 0.317 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 705 Number of extensions: 14 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 480 Length of database: 482 Length adjustment: 34 Effective length of query: 446 Effective length of database: 448 Effective search space: 199808 Effective search space used: 199808 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory