Alignments for a candidate for fruI in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Alignments for a candidate for fruI in Cupriavidus basilensis 4G11

Align Phosphoenolpyruvate--protein phosphotransferase (EC 2.7.3.9) (characterized)
to candidate RR42_RS01795 RR42_RS01795 phosphoenolpyruvate-protein phosphotransferase

Query= reanno::pseudo1_N1B4:Pf1N1B4_1146
         (953 letters)



>FitnessBrowser__Cup4G11:RR42_RS01795
          Length = 591

 Score =  313 bits (803), Expect = 2e-89
 Identities = 201/587 (34%), Positives = 306/587 (52%), Gaps = 35/587 (5%)

Query: 402 IQAIAAAPGIAIGPAHIQVQQVID---YPLRGESAAIERERLKQALADVRRDIEGL---I 455
           +  I  + G+AIG AH+     +D   Y +  +    E ERL+ A A VR ++  L   +
Sbjct: 5   LHGIPVSRGVAIGRAHLLAPAALDVSHYLVDEDRLDAEVERLRSARAAVRAELVTLKRDL 64

Query: 456 ERSKAKAIREIFITHQEMLDDPELTDEVDTRLKQGE-SAEAAWMAVIEAAAKQQESLQDA 514
            R   + +      H  +LDD  L  E +  ++    +AE A    +E   +Q + ++D 
Sbjct: 65  PREAPEELGAFLDVHAMILDDEALAREPEALIRNRRYNAEWALTTRLEELMRQFDEIEDE 124

Query: 515 LLAERAADLRDIGRRVLAQLSG--VETPA-----------EPEQPYILVMDEVGPSDVAR 561
            L ER AD+R +  R+L  L+G  V  PA           EP    I+V  ++ P+D+ +
Sbjct: 125 YLRERKADIRQVVERILKVLAGAAVLAPAPVPPSSLAPDGEPAPGVIVVAHDISPADMLQ 184

Query: 562 LDPTRVAGILTARGGATAHSAIVARALGIPALVGAGAAVLLLKPGTPLLIDGQRGRLHVD 621
              T   G +T  GG T+H+AIVAR+L IPA VG  +A  L++    ++IDG  G + VD
Sbjct: 185 FRHTVFHGFVTDLGGRTSHTAIVARSLDIPAAVGVQSASELIRQDDWVIIDGDAGLVIVD 244

Query: 622 ADAATLQRATEERDTRELRLKAAAEQRHQPALTTDGHAVEVFANIGESAGVTSAVEQGAE 681
             A  L+     +  R L  K     RH PA+T DG  +++ ANI  +   T+A+  GA 
Sbjct: 245 PSAIILEEYRHRQSERALEKKRLQRLRHTPAVTLDGLEIDLLANIEMAEDATAALTAGAV 304

Query: 682 GIGLLRTELIFM-AHSQAPDEATQEVEYRRVLDGLAGRPLVVRTLDVGGDKPLPYWP--- 737
           G+GL R+E +FM    + PDE  Q   YR  +D + G P+ +RT+D+G DKPL       
Sbjct: 305 GVGLFRSEFLFMNRRGELPDEEEQFEAYRGAVDAMHGLPVTIRTIDIGADKPLDGRDGRD 364

Query: 738 -----IAKEENPFLGVRGIRLTLQRPQVMEAQLRALLRAADNRPLRIMFPMVGSVDEWRQ 792
                     NP LG+R IR +L  P +   QLRALLRA+   P+R++ PM+    E  Q
Sbjct: 365 GRGDDFETALNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGPVRLLIPMLAHAREIDQ 424

Query: 793 ARDMTERLRLEIPVA------DLQLGIMIEVPSAALLAPVLAKEVDFFSVGTNDLTQYTL 846
             D+  + + ++         ++++G MIE+P+A L+ P+  + +DF S+GTNDL QYTL
Sbjct: 425 TLDLIAQAKRQLDARGQAFDPNIKVGAMIEIPAAVLMLPLFLRRMDFLSIGTNDLIQYTL 484

Query: 847 AIDRGHPTLSAQADGLHPAVLQLIDITVRAAHAHGKWVGVCGELAADPLAVPVLVGLGVD 906
           AIDR    ++   D LHPAVLQLI  T+R A+  G  V VCGE+A DP    +L+G+G+ 
Sbjct: 485 AIDRADNAVAHLYDPLHPAVLQLIARTIREANRAGVPVAVCGEMAGDPAMTRLLLGMGLR 544

Query: 907 ELSVSGRSIAEVKARIRELSLTQTQTLAQQALAVGSANEVRALVEAL 953
           E S+    +  VK  +      + + L +Q L+     E+   ++ L
Sbjct: 545 EFSMHPSQLLRVKQEVLHADCERLERLVEQVLSAYEPEELACALKLL 591


Lambda     K      H
   0.318    0.135    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1154
Number of extensions: 45
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 953
Length of database: 591
Length adjustment: 40
Effective length of query: 913
Effective length of database: 551
Effective search space:   503063
Effective search space used:   503063
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources