Alignments for a candidate for iolA in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Alignments for a candidate for iolA in Cupriavidus basilensis 4G11

Align malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18); methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized)
to candidate RR42_RS28560 RR42_RS28560 methylmalonate-semialdehyde dehydrogenase

Query= BRENDA::Q02252
         (535 letters)



>FitnessBrowser__Cup4G11:RR42_RS28560
          Length = 506

 Score =  596 bits (1536), Expect = e-175
 Identities = 282/496 (56%), Positives = 380/496 (76%), Gaps = 1/496 (0%)

Query: 29  SASSFSSSVPTVKLFIGGKFVESKSDKWIDIHNPATNEVIGRVPQATKAEMDAAIASCKR 88
           SA + +++ PTVKL I G++VES + ++  + NPAT +V+ +VP AT AE++AA+ +  R
Sbjct: 2   SAQNPNAAAPTVKLLINGEWVESAATEFRPVVNPATQQVLAQVPLATAAEVNAAVGAAHR 61

Query: 89  AFPAWADTSVLSRQQVLLRYQQLIKENLKEIAKLITLEQGKTLADAEGDVFRGLQVVEHA 148
           AF  W  T + +R +++L+YQ LI+E+ K IA ++T EQGKTLADAEGD+FRGL+VVEHA
Sbjct: 62  AFATWRHTPLGARLRIMLKYQALIREHSKRIAAILTAEQGKTLADAEGDIFRGLEVVEHA 121

Query: 149 CSVTSLMMGETMPSITKDMDLYSYRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTFLM 208
           CS+ +L  G    ++   +D Y+ + P+GVCAGI PFNFPAMIPLWMFPMA+VCGNTF++
Sbjct: 122 CSIGTLQQGGFAENVAATVDTYTLQQPIGVCAGITPFNFPAMIPLWMFPMAIVCGNTFVL 181

Query: 209 KPSERVPGATMLLAKLLQDSGAPDGTLNIIHGQHEAVNFICDHPDIKAISFVGSNKAGEY 268
           KPSE+ P +TM L KL  ++G P G LN++HG  E V+ +C HPDIKA+SFVGS   G +
Sbjct: 182 KPSEQDPLSTMELVKLAIEAGVPAGVLNVVHGAREVVDALCTHPDIKAVSFVGSTAVGTH 241

Query: 269 IFERGSRHGKRVQANMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAVLVGE 328
           ++     HGKRVQ+ MGAKNH VV+PDA++E+TLN L GAAFGAAGQRCMA S AVLVG 
Sbjct: 242 VYNLAGAHGKRVQSMMGAKNHAVVLPDAHREHTLNALAGAAFGAAGQRCMATSVAVLVGA 301

Query: 329 AKKWLPELVEHAKNLRVNAGDQPGADLGPLITPQAKERVCNLIDSGTKEGASILLDGRKI 388
           A++WLPELV+ A  L+V AG++PG D+ P+++  A++RV  LI++G +EGA++ LDGR +
Sbjct: 302 AREWLPELVKKAATLKVGAGNEPGTDVCPVVSVAARQRVLGLIEAGEREGATLALDGRNV 361

Query: 389 KVKGYENGNFVGPTIISNVKPNMTCYKEEIFGPVLVVLETETLDEAIQIVNNNPYGNGTA 448
           +V GY  GNF+GPT+ ++V   M+ Y +EIFGPVLVV+  ETLDEAI +VN NP+GNGT 
Sbjct: 362 QVAGYPEGNFIGPTVFADVTTEMSIYTQEIFGPVLVVIGVETLDEAIALVNRNPFGNGTG 421

Query: 449 IFTTNGATARKYAHLVDVGQVGVNVPIPVPLPMFSFTGSRSSFRGDTNFYGKQGIQFYTQ 508
           IFT +GA ARK+   +DVGQVG+N+PIPVP+P FSFTGSR S  GD   YGKQ +QFYTQ
Sbjct: 422 IFTQSGAAARKFQSEIDVGQVGINIPIPVPVPYFSFTGSRGSKLGDLGPYGKQVVQFYTQ 481

Query: 509 LKTITSQWKEEDATLS 524
            KT+T++W  +DAT++
Sbjct: 482 TKTVTARW-FDDATVN 496


Lambda     K      H
   0.318    0.133    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 684
Number of extensions: 29
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 535
Length of database: 506
Length adjustment: 35
Effective length of query: 500
Effective length of database: 471
Effective search space:   235500
Effective search space used:   235500
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources