Align L-threonine 3-dehydrogenase (EC 1.1.1.103) (characterized)
to candidate RR42_RS28300 RR42_RS28300 NAD-dependent epimerase
Query= reanno::Cup4G11:RR42_RS28300 (324 letters) >FitnessBrowser__Cup4G11:RR42_RS28300 Length = 324 Score = 671 bits (1732), Expect = 0.0 Identities = 324/324 (100%), Positives = 324/324 (100%) Query: 1 MTKPARVLIIGANGQLGTELASALADRYGKQNVVTSDMVPHGRHLHLTHEMLDVTDRAHL 60 MTKPARVLIIGANGQLGTELASALADRYGKQNVVTSDMVPHGRHLHLTHEMLDVTDRAHL Sbjct: 1 MTKPARVLIIGANGQLGTELASALADRYGKQNVVTSDMVPHGRHLHLTHEMLDVTDRAHL 60 Query: 61 RDVIERHGITQIYHLAAALSATGEKSPTWAWQLNMNGLLNVLEAARHQKLDKVFWPSSIA 120 RDVIERHGITQIYHLAAALSATGEKSPTWAWQLNMNGLLNVLEAARHQKLDKVFWPSSIA Sbjct: 61 RDVIERHGITQIYHLAAALSATGEKSPTWAWQLNMNGLLNVLEAARHQKLDKVFWPSSIA 120 Query: 121 AFGPTTPPDGTPQSTIMEPKTVYGISKLAGEGWCRWYFENHGVDVRSLRYPGLISYKTPP 180 AFGPTTPPDGTPQSTIMEPKTVYGISKLAGEGWCRWYFENHGVDVRSLRYPGLISYKTPP Sbjct: 121 AFGPTTPPDGTPQSTIMEPKTVYGISKLAGEGWCRWYFENHGVDVRSLRYPGLISYKTPP 180 Query: 181 GGGTTDYAIDIFHSALRAQPYACFLEKDEALPMMYMPDAVRATMELMEAPRASISERGSY 240 GGGTTDYAIDIFHSALRAQPYACFLEKDEALPMMYMPDAVRATMELMEAPRASISERGSY Sbjct: 181 GGGTTDYAIDIFHSALRAQPYACFLEKDEALPMMYMPDAVRATMELMEAPRASISERGSY 240 Query: 241 NLAGLSFTPGEIANEIRRHCPGFDVRYEPDFRQEIAAGWPDSIDDSVARRDWNWRPEFGL 300 NLAGLSFTPGEIANEIRRHCPGFDVRYEPDFRQEIAAGWPDSIDDSVARRDWNWRPEFGL Sbjct: 241 NLAGLSFTPGEIANEIRRHCPGFDVRYEPDFRQEIAAGWPDSIDDSVARRDWNWRPEFGL 300 Query: 301 KEMVADMLKNLATLNQGSALECVS 324 KEMVADMLKNLATLNQGSALECVS Sbjct: 301 KEMVADMLKNLATLNQGSALECVS 324 Lambda K H 0.319 0.135 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 580 Number of extensions: 15 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 324 Length of database: 324 Length adjustment: 28 Effective length of query: 296 Effective length of database: 296 Effective search space: 87616 Effective search space used: 87616 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory