Align trehalose-specific PTS system, I, HPr, and IIA components (characterized)
to candidate RR42_RS01795 RR42_RS01795 phosphoenolpyruvate-protein phosphotransferase
Query= reanno::pseudo3_N2E3:AO353_15995 (844 letters) >FitnessBrowser__Cup4G11:RR42_RS01795 Length = 591 Score = 252 bits (644), Expect = 4e-71 Identities = 181/590 (30%), Positives = 285/590 (48%), Gaps = 40/590 (6%) Query: 277 LRGVCASAGSAFGYVVQVAERTLEMPEFAADQ-----QLERESLERALMHATQA-LQRLR 330 L G+ S G A G +A L++ + D+ ++ER RA + A L+R Sbjct: 5 LHGIPVSRGVAIGRAHLLAPAALDVSHYLVDEDRLDAEVERLRSARAAVRAELVTLKRDL 64 Query: 331 DNAAGEAQADIFKAHQELLEDPSLLEQAQALIAEGK-SAAFAWNSATEATATLFKSLGST 389 A E H +L+D +L + +ALI + +A +A + E F + Sbjct: 65 PREAPEELGAFLDVHAMILDDEALAREPEALIRNRRYNAEWALTTRLEELMRQFDEIEDE 124 Query: 390 LLAERALDLMDVGQRVLKLILGV--------------PDGVWELPDQAILIAEQLTPSQT 435 L ER D+ V +R+LK++ G PDG E I++A ++P+ Sbjct: 125 YLRERKADIRQVVERILKVLAGAAVLAPAPVPPSSLAPDG--EPAPGVIVVAHDISPADM 182 Query: 436 AALDTGKVLGFATVGGGATSHVAILARALGLPAVCGLPLQVLSLASGTRVLLDADKGELH 495 GF T GG TSH AI+AR+L +PA G+ + V++D D G + Sbjct: 183 LQFRHTVFHGFVTDLGGRTSHTAIVARSLDIPAAVGVQSASELIRQDDWVIIDGDAGLVI 242 Query: 496 LDPAVSVIEQLHAKRQQQRQRHQHELENAARA-AVTRDGHHFEVTANVASLAETEQAMSL 554 +DP+ ++E+ + RQ +R + L+ AVT DG ++ AN+ + A++ Sbjct: 243 VDPSAIILEE-YRHRQSERALEKKRLQRLRHTPAVTLDGLEIDLLANIEMAEDATAALTA 301 Query: 555 GAEGIGLLRSEFLYQ-QRSVAPSHDEQAGTYSAIARALGPQRNLVVRTLDVGGDKPL--- 610 GA G+GL RSEFL+ +R P +EQ Y A+ + +RT+D+G DKPL Sbjct: 302 GAVGVGLFRSEFLFMNRRGELPDEEEQFEAYRGAVDAMHGLP-VTIRTIDIGADKPLDGR 360 Query: 611 -----AYVPMDSEANPFLGMRGIRLCLERPQLLREQFRAILSSAGLARLHIMLPMVSQLS 665 ++ NP LG+R IR L P + Q RA+L ++ + +++PM++ Sbjct: 361 DGRDGRGDDFETALNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGPVRLLIPMLAHAR 420 Query: 666 ELR-----LARLMLEEEALALGLRELPKLGIMIEVPAAALMADLFAPEVDFFSIGTNDLT 720 E+ +A+ + +A K+G MIE+PAA LM LF +DF SIGTNDL Sbjct: 421 EIDQTLDLIAQAKRQLDARGQAFDPNIKVGAMIEIPAAVLMLPLFLRRMDFLSIGTNDLI 480 Query: 721 QYTLAMDRDHPRLASQADSFHPSVLRLIASTVKAAHAHGKWVGVCGALASETLAVPLLLG 780 QYTLA+DR +A D HP+VL+LIA T++ A+ G V VCG +A + LLLG Sbjct: 481 QYTLAIDRADNAVAHLYDPLHPAVLQLIARTIREANRAGVPVAVCGEMAGDPAMTRLLLG 540 Query: 781 LGVDELSVSVPLIPAIKAAIREVELSDCQAIAHQVLGLESAEQVREALSV 830 +G+ E S+ + +K + + + + QVL E++ AL + Sbjct: 541 MGLREFSMHPSQLLRVKQEVLHADCERLERLVEQVLSAYEPEELACALKL 590 Lambda K H 0.318 0.132 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1056 Number of extensions: 52 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 844 Length of database: 591 Length adjustment: 39 Effective length of query: 805 Effective length of database: 552 Effective search space: 444360 Effective search space used: 444360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory