Align Anthranilate 1,2-dioxygenase small subunit; EC 1.14.12.1 (characterized)
to candidate RR42_RS33935 RR42_RS33935 salicylate hydroxylase
Query= SwissProt::Q84BZ2 (161 letters) >FitnessBrowser__Cup4G11:RR42_RS33935 Length = 157 Score = 107 bits (268), Expect = 7e-29 Identities = 49/156 (31%), Positives = 91/156 (58%), Gaps = 2/156 (1%) Query: 7 DMKTWFEIYMLQNRYIGHLDNDRLERWPEMFTEDCTYEIVPKENADLGLPVGIVHCTNQR 66 D +T+ ++ L Y LDN+ E+WP FTE C Y++ P+EN + GLP+ + ++ Sbjct: 3 DFETYTQVLALYTDYACALDNNEWEKWPGFFTEACVYKLQPRENHEAGLPLATLCFESRG 62 Query: 67 MLRDRVVSLRHANIYEEHTYRHMTSGLAIVAQRDGE-IDTESNYVVVQTRSNGESNVYQA 125 ML+DR+ ++ + RH+ G+ + D + ID E++Y V +T+ +G++ VY Sbjct: 63 MLKDRIYGATETIFHDPYYQRHVV-GIPRILNADAQRIDAEASYAVFRTKPDGQTTVYNV 121 Query: 126 GKYYDTVVRTPDGLRYKAKRVIYDTSRVQTLLATPI 161 G+Y D + RT DGL+++++ ++D+ + + PI Sbjct: 122 GRYIDVIRRTGDGLKFESRLCVFDSEMIANSIIYPI 157 Lambda K H 0.319 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 77 Number of extensions: 3 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 161 Length of database: 157 Length adjustment: 17 Effective length of query: 144 Effective length of database: 140 Effective search space: 20160 Effective search space used: 20160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 43 (21.2 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory