Align aminomuconate-semialdehyde dehydrogenase (EC 1.2.1.32) (characterized)
to candidate RR42_RS26255 RR42_RS26255 aldehyde dehydrogenase
Query= BRENDA::Q83XU8 (485 letters) >FitnessBrowser__Cup4G11:RR42_RS26255 Length = 499 Score = 342 bits (876), Expect = 2e-98 Identities = 187/465 (40%), Positives = 265/465 (56%), Gaps = 11/465 (2%) Query: 24 SPLNNAVIAKVHEAGRAEVDAAVAAAQAALK-GAWGRMSLAQRVEVLYAVADGINRRFDD 82 +P +A+V G +VD AVAAA+ A + G W + R L ++ I ++ Sbjct: 42 NPATGKALARVAACGAQDVDRAVAAARKAFQSGVWSDTPRSARKATLLRLSHLIETHREE 101 Query: 83 FLAAEVEDTGKPMSLARHVDIPRGAANFKIFADVVKNVPTEFFEMPTPDGVGAINYAVRR 142 E D GKP++ DIP + +A+ + + E P G G + R Sbjct: 102 LALLETLDMGKPIAETLQYDIPEAGRTYAWYAEAIDKIYDEI----APTGPGVLATITRE 157 Query: 143 PVGVVGVICPWNLPLLLMTWKVGPALACGNTVVVKPSEETPQTAALLGEVMNTAGVPPGV 202 P+GVV + PWN PLL+ +WKV PALA GN+VV+KP+E++P TA L E+ AG+P GV Sbjct: 158 PLGVVAAVVPWNYPLLMASWKVAPALAAGNSVVLKPAEQSPLTALRLAELAEEAGIPAGV 217 Query: 203 YNVVHGFGPNSTGEFLTSHPDVNAITFTGETGTGEAIMK-AAADGARPVSLELGGKNAAI 261 +NVV G G + G+ L HPDV+ I FTG T TG+ M+ + + V LE GGK+ I Sbjct: 218 FNVVPGLGA-AAGQALGRHPDVDCIAFTGSTATGKRFMEYSGQSNLKRVWLECGGKSPHI 276 Query: 262 VFADC-DLDKAIEGTLRSCFANCGQVCLGTERVYVERPIFDRFVSRLKKGAEGMQLGRPE 320 VF DC DLD+A + F+N G++C+ R+YV+ I+D F+ +L+ A MQ G P Sbjct: 277 VFDDCPDLDRAAQAAAIGIFSNQGEICIAGSRLYVQSGIYDAFMEKLEAHAARMQPGDPL 336 Query: 321 DLATGMGPLISQEHREKVLSYYKKAVEAGATVVTGGGVPEMPEALKGGAWVQPTIWTGLG 380 D AT MG ++ ++V+SY K + GA + GG + GG ++QPTI+ Sbjct: 337 DPATAMGAIVDGAQLQRVMSYVKSGEDEGARLRAGG---KRAHTDSGGFYMQPTIFECPT 393 Query: 381 DDSVVAREEIFGPCALVMPFDSEEEVIRRANDNDYGLARRIWTTNLSRAHRVAGAIEVGI 440 + REEIFGP V F+SEEE IR AND+ YGL +WT+NLSRAHRV+ ++ G+ Sbjct: 394 QSLTIVREEIFGPVLAVTRFESEEEAIRMANDSPYGLGSGLWTSNLSRAHRVSRKLQAGL 453 Query: 441 AWVNSWFLRDLRTAFGGSKQSGIGREGGVHSLEFYTELKNVCIKL 485 WVN + D+ FGG KQSG GR+ +H+L+ YT+LK I L Sbjct: 454 VWVNCYMDSDVTVPFGGVKQSGSGRDKSLHALDKYTDLKTTWISL 498 Lambda K H 0.318 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 594 Number of extensions: 34 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 485 Length of database: 499 Length adjustment: 34 Effective length of query: 451 Effective length of database: 465 Effective search space: 209715 Effective search space used: 209715 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory