Aligments for a candidate for Ch1CoA in Dinoroseobacter shibae DFL-12

GapMind for catabolism of small carbon sources

Aligments for a candidate for Ch1CoA in Dinoroseobacter shibae DFL-12

Align Cyclohex-1-ene-1-carbonyl-CoA dehydrogenase; Ch1CoA; EC 1.3.8.10 (characterized)
to candidate 3607424 Dshi_0838 acyl-CoA dehydrogenase domain protein (RefSeq)

Query= SwissProt::Q2LQN9
         (414 letters)



>lcl|FitnessBrowser__Dino:3607424 Dshi_0838 acyl-CoA dehydrogenase
           domain protein (RefSeq)
          Length = 382

 Score =  234 bits (597), Expect = 3e-66
 Identities = 148/381 (38%), Positives = 211/381 (55%), Gaps = 12/381 (3%)

Query: 37  LTEEQKLLMEMVRNLAVREIAPRAIEIDENHSFPVHARDLFADLGLLSPLVPVEYGGTGM 96
           +T+E ++L EM RN    E AP      +             +LGLL P VP  YGG G 
Sbjct: 7   MTDEHRMLAEMTRNFITTEWAPHFERWRDQGEMDREIWQQAGELGLLCPSVPEAYGGPGG 66

Query: 97  DITTFAMVLEEIGKVCASTALMLLAQADGMLS--IILDGSPALKEKYLPRFGEKSTLMTA 154
           D    A +L EI +   S          G+++  I+  GS   K+K+LP+      ++ A
Sbjct: 67  DFGHEAAILIEIARANLSAWGAGHGIHSGIVAHYILAYGSEEQKQKWLPKM-VSGEMVGA 125

Query: 155 FAATEPGAGSDLLAMKTRAVKKGDKYVINGQKCFITNGSVADILTVWAYTDPSKGAKGMS 214
            A TEPGAGSDL  +KTRAVK G+ Y ++G K FITNG  A+++ V A TDPS GAKG+S
Sbjct: 126 LAMTEPGAGSDLQGIKTRAVKDGNGYRLSGSKIFITNGQHANLIVVAAKTDPSAGAKGVS 185

Query: 215 TFVVE-RGTPGLIYGHNEKKMGMRGCPNSELFFEDLEVPAENLV-GEEGKGFAYLMGALS 272
             V+E  G  G   G N  K+GM     SELFF+++ +P ENL+ GE GKGF  +M  L 
Sbjct: 186 LVVLETEGAEGFSRGRNLHKVGMHASDTSELFFDNVAIPPENLLGGEVGKGFYQMMTQLP 245

Query: 273 INRVFCASQAVGIAQGALERAMQHTREREQFGKPIAHLTPIQFMIADMATEVEAARLLVR 332
             R+  A+ AVG  +GA+ER + + +ER+ FG PI      +F +A+  T+   AR  + 
Sbjct: 246 QERLIIAAGAVGAMEGAVERTVAYAKERQAFGGPILQFQNTRFKLAECQTKTTVARAFLN 305

Query: 333 KATTLLDAKDKRGPLI---GGMAKTFASDTAMKVTTDAVQVMGGSGYMQEYQVERMMREA 389
           +      A+   G L      MAK + +DT  +V  + VQ+ GG GYM EY + +M  +A
Sbjct: 306 ECM----AEHLEGKLSVEKAAMAKYWITDTQGEVIDECVQLHGGYGYMAEYDIAQMWSDA 361

Query: 390 KLTQIYTGTNQITRMVTGRSL 410
           ++ +IY GTN+I + + GR+L
Sbjct: 362 RVQRIYGGTNEIMKELIGRAL 382


Lambda     K      H
   0.318    0.133    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 384
Number of extensions: 20
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 414
Length of database: 382
Length adjustment: 31
Effective length of query: 383
Effective length of database: 351
Effective search space:   134433
Effective search space used:   134433
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources