GapMind for catabolism of small carbon sources

 

Aligments for a candidate for aldA in Dinoroseobacter shibae DFL-12

Align lactaldehyde dehydrogenase (EC 1.2.1.22); D-glyceraldehyde dehydrogenase (NADP+) (EC 1.2.1.89) (characterized)
to candidate 3609503 Dshi_2887 succinic semialdehyde dehydrogenase (RefSeq)

Query= BRENDA::P25553
         (479 letters)



>lcl|FitnessBrowser__Dino:3609503 Dshi_2887 succinic semialdehyde
           dehydrogenase (RefSeq)
          Length = 492

 Score =  296 bits (758), Expect = 1e-84
 Identities = 171/470 (36%), Positives = 257/470 (54%), Gaps = 4/470 (0%)

Query: 10  YIDGQFVTWRGDAWIDVVNPATEAVISRIPD-GQAEDARKAIDAAERAQPEWEALPAIER 68
           Y  G +      A   V NPA   VI+ +PD G+AE AR AI AA+ AQ  W A  A +R
Sbjct: 23  YFAGAWTDADSGATFPVTNPARGDVIAHVPDLGRAETAR-AIAAADAAQKPWAARTAKDR 81

Query: 69  ASWLRKISAGIRERASEISALIVEEGGKIQQLAEVEVAFTADYIDYMAEWARRYEGEIIQ 128
           A  LR+    I   A +++ ++  E GK    A  EV + A ++++ AE A+R  GE I 
Sbjct: 82  AQVLRRWFDLIVGNADDLARILTAEMGKPLAEARGEVMYGASFVEWFAEEAKRLYGETIP 141

Query: 129 SDRPGENILLFKRALGVTTGILPWNFPFFLIARKMAPALLTGNTIVIKPSEFTPNNAIAF 188
              P   I + ++ +GV   I PWNFP  +I RK APAL  G   + KP+E TP +A+A 
Sbjct: 142 GHLPDARIQVIRQPIGVVGAITPWNFPIAMITRKAAPALAAGCAFLSKPAEDTPLSALAL 201

Query: 189 AKIVDEIGLPRGVFN-LVLGRGETVGQELAGNPKVAMVSMTGSVSAGEKIMATAAKNITK 247
           A + +  G+P G+F  L       +G+E   N  V  ++ TGS   G  ++A AA  + K
Sbjct: 202 AVLAERAGIPAGLFAVLPSSDSSAIGKEFCENHTVRKLTFTGSTQVGRILLAQAADQVKK 261

Query: 248 VCLELGGKAPAIVMDDADLELAVKAIVDSRVINSGQVCNCAERVYVQKGIYDQFVNRLGE 307
             +ELGG AP IV DDADL+ AV+  +  +  N+GQ C CA R+YVQ G+YD F  +L  
Sbjct: 262 CSMELGGNAPFIVFDDADLDKAVEGAMACKFRNAGQTCVCANRIYVQDGVYDAFAEKLAA 321

Query: 308 AMQAVQFGNPAERNDIAMGPLINAAALERVEQKVARAVEEGARVAFGGKAVEGKGYYYPP 367
           A++ ++ G+ A    + +GPLIN  A+E+V+  +     +G  V  GG+     G ++ P
Sbjct: 322 AVEELKVGDGAAEG-VTIGPLINMPAVEKVQDHLDDLRAKGGTVVTGGETHPLGGTFFTP 380

Query: 368 TLLLDVRQEMSIMHEETFGPVLPVVAFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAI 427
           T++  V QEM +  EETFGPV P+  F   ++ I+MAND+ +GL    Y +++    +  
Sbjct: 381 TVVTGVTQEMKVAREETFGPVAPLFRFTEEDEVIAMANDTIFGLAGYFYARDIGRITRVS 440

Query: 428 KGLKFGETYINRENFEAMQGFHAGWRKSGIGGADGKHGLHEYLQTQVVYL 477
           + L++G   IN            G ++SG+G    +HG+ EYL+ + + L
Sbjct: 441 EALEYGIVGINTGIISTEGAPFGGVKQSGLGREGSRHGIDEYLEMKYICL 490


Lambda     K      H
   0.318    0.135    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 550
Number of extensions: 25
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 479
Length of database: 492
Length adjustment: 34
Effective length of query: 445
Effective length of database: 458
Effective search space:   203810
Effective search space used:   203810
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory