Alignments for a candidate for xacD in Dinoroseobacter shibae DFL-12

GapMind for catabolism of small carbon sources

Alignments for a candidate for xacD in Dinoroseobacter shibae DFL-12

Align L-arabonate dehydratase (EC 4.2.1.25) (characterized)
to candidate 3607836 Dshi_1244 Dihydroxy-acid dehydratase (RefSeq)

Query= reanno::BFirm:BPHYT_RS19730
         (577 letters)



>FitnessBrowser__Dino:3607836
          Length = 569

 Score =  717 bits (1850), Expect = 0.0
 Identities = 361/568 (63%), Positives = 437/568 (76%), Gaps = 6/568 (1%)

Query: 12  RSQAWFGLKDRDGFLHRSWMKNQGIPHDEFDGRPVIGICNTWSELTPCNAHFRELAEYVK 71
           R++AW+G  D+DGF+HRSWMKNQG P   FDGRPVIGICNTWSELTPCN   R LAE VK
Sbjct: 6   RNRAWYGKLDKDGFIHRSWMKNQGFPDHVFDGRPVIGICNTWSELTPCNHGLRTLAEGVK 65

Query: 72  KGVHEAGGLPLEFPVMSLGETNLRPTAMLFRNLASMDVEESIRGNPMDGVILLVGCDKTT 131
           +GV EAGG P+EFPVMSLGET ++PTAMLFRNL +MDVEESIR   MDGV+LL GCDKTT
Sbjct: 66  RGVWEAGGFPVEFPVMSLGETQMKPTAMLFRNLLAMDVEESIRAYGMDGVVLLGGCDKTT 125

Query: 132 PALLMGAASCNLPALAVSGGPMLNGRFRGKNIGSGTGVWQMSEEVRAGTMTQEEFTEAES 191
           P  LMGAAS +LP++ VS GPMLNG++RG++IGSGT VW+ SE VRAG MT  +F  AE+
Sbjct: 126 PGQLMGAASVDLPSIVVSAGPMLNGKYRGQDIGSGTDVWKFSEAVRAGEMTLADFMNAEA 185

Query: 192 CMNRSRGHCMTMGTASTMASMVESLGMGLPHNAAIPAVDARRQVLAHLAGRRIVDMVRED 251
            M+RS G CMTMGTASTMAS+VE++GM LP NAA+PAVDARR  LAHL G+RIV+MV ED
Sbjct: 186 GMSRSAGVCMTMGTASTMASLVEAMGMSLPLNAALPAVDARRMALAHLTGKRIVEMVEED 245

Query: 252 LTMDKILTRQAFENAIRTNAAIGGSTNAVVHLIALAKRIGVELSLEDWELGSNVPCLVNL 311
           L    ILT+ AFENAI  NAA+GGSTNAV+HL+ALA R GV+L+L+D+E+G  +P LVN 
Sbjct: 246 LRPSAILTKPAFENAILANAAVGGSTNAVMHLLALAGRAGVDLTLKDFEIGGEIPLLVNC 305

Query: 312 QPSGEYLMEDFYYAGGLPAVLKQLGEQGLLHKEALTVNGKTLWDNVRNAANYDEKVITTF 371
            PSG+YLMEDF YAGG+P VL +L +     + A TV GK +      A  ++  VI  +
Sbjct: 306 MPSGKYLMEDFAYAGGMPVVLSELRDH---LRPATTVLGKDIAAYTEGAECFNRDVIHAY 362

Query: 372 AEPFKPKAGIAVLKGNLAPNGAVIKPSAATASLLKHRGRAVVFENIEELHAKIDDESLDI 431
             P KP AG+ VL+G+LAP+GA++KPSAAT +LL+H G A VFENIE++ A ID + L I
Sbjct: 363 DAPVKPAAGLRVLRGSLAPDGAIVKPSAATDALLEHEGPAFVFENIEDMKANIDRDDLPI 422

Query: 432 DEHCIMVLKGAGPKGYPGFAEVGNMPLPKKVLQKGITDMVRISDGRMSGTAYGAVVLHVS 491
               I+VLKG GPKGYPG  EVGNMP+P K++++G+ DM+R+SD RMSGTAYG V+LHV+
Sbjct: 423 TPDTILVLKGCGPKGYPGMPEVGNMPIPAKLVREGVRDMIRVSDARMSGTAYGTVILHVA 482

Query: 492 PEAAAGGPLAFVQTGDMIELDVEERRLHLDVTDEELARRRAAWQAPEAP--KRGYYKLYV 549
           PEA AGGPLA V+TGD I +   E  L L V   ELA RRAAWQ P+ P   RGY KLY+
Sbjct: 483 PEAQAGGPLALVKTGDRIRVSAREGVLDLLVDATELAARRAAWQ-PDPPHYTRGYAKLYI 541

Query: 550 EHVLQADQGADLDFLVGSSGAPVPRDSH 577
           +HVLQAD+GADLDFLVG    PV R+SH
Sbjct: 542 DHVLQADRGADLDFLVGKDTRPVTRESH 569


Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1007
Number of extensions: 41
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 577
Length of database: 569
Length adjustment: 36
Effective length of query: 541
Effective length of database: 533
Effective search space:   288353
Effective search space used:   288353
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources