Alignments for a candidate for putA in Dinoroseobacter shibae DFL-12

GapMind for catabolism of small carbon sources

Alignments for a candidate for putA in Dinoroseobacter shibae DFL-12

Align L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88) (characterized)
to candidate 3609634 Dshi_3017 aldehyde dehydrogenase (RefSeq)

Query= BRENDA::P07275
         (575 letters)



>FitnessBrowser__Dino:3609634
          Length = 780

 Score =  114 bits (284), Expect = 2e-29
 Identities = 126/480 (26%), Positives = 201/480 (41%), Gaps = 71/480 (14%)

Query: 79  FPQTNPANHQQVLANVTQATEKDVMNAVKAAKDAKKDWYNLPFYDRSAIFLKAADLISTK 138
           F   NPA   + LA VTQ TE D+  AV AA+ A+  W  L     + +    A L+   
Sbjct: 48  FATRNPARGTE-LAQVTQGTEADIEAAVTAARRAQPAWARLGGQGWARVLYAIARLLQKH 106

Query: 139 YRYDMLAATMLGQGKNVYQAEIDCITELSDFFRYYVKYASDLYAQQPVESADGTWNKAEY 198
            R   +  T L  GK + ++    I      F Y+   A+ +  + P     G   +   
Sbjct: 107 SRLFAVLET-LDNGKPIRESRDIDIPLAQRHFYYHAGMAALMEDELPDRVPVGVCGQ--- 162

Query: 199 RPLEGFVYAVSPFNFTAIAANLIGAPAL-MGNTVVWKPSQTAALSNYLLMTVLEEAGLPK 257
                    + P+NF  +      APAL MGNTVV KP++   L+  L   +  EAG+P 
Sbjct: 163 ---------IVPWNFPLLMLAWKIAPALAMGNTVVLKPAEWTPLTALLFAEICGEAGVPP 213

Query: 258 GVINFIPGDPVQVTDQVLADKDFGALHFTGSTNVFKSLYGKIQSGVVEGKYRDYPRIIGE 317
           GV+N + GD       +++      + FTGST+V + +    ++    GK      +  E
Sbjct: 214 GVVNIVTGDGA-TGAALVSHPGVDKIAFTGSTDVGREIR---RATAGTGK-----ALTLE 264

Query: 318 TGGKNFHLVHPSANISHAVLSTIRGTFEFQGQKCSAASRLYLPESKSEEFLSDMFGILQS 377
            GGK+ ++V   A++  AV   +   +  QGQ C A SRL + E  +E F +        
Sbjct: 265 LGGKSPYVVFEDADLDSAVEGLVDAIWFNQGQVCCAGSRLLVQEGIAEAFYA-------- 316

Query: 378 QNVVPMNTSASPISGGNLRGFMGPVIHEQSFDKLVKV---IEDAKKDPELEILYGGQYD- 433
                            LR  M  +      DK + V   ++ A      E++ G   + 
Sbjct: 317 ----------------KLRARMATLRIGDPLDKCIDVGAMVDPAHCAKVAEMVAGSNGEV 360

Query: 434 ------KSQGWFVGPTVIKAKRPDHPYMSTEFFGPILTVYEYPDTEFNEICDIID--NTS 485
                  ++G F  PT+I    P  P M  E FGP+L       T F    + ++  N +
Sbjct: 361 HQPGTVPAEGAFYPPTLITGLEPADPLMRDEIFGPVLVA-----TTFRTPAEAVEVANAT 415

Query: 486 QYALTGAIFAKD-RKAIEYADEKLKFSAGNFYINDKCTGAVVSQQWFGGARMSGTDDKAG 544
           +Y L   +++++  +A++ A    K +AG  ++N   T    +   FGG R SG   + G
Sbjct: 416 RYGLAATLWSENINRALDIAP---KLAAGVVWVN--ATNLFDAAAGFGGVRESGFGREGG 470



 Score = 47.0 bits (110), Expect = 3e-09
 Identities = 58/233 (24%), Positives = 104/233 (44%), Gaps = 15/233 (6%)

Query: 90  VLANVTQATEKDVMNAVKAAKDAKKDWYNLPFYDRSAIFLKAADLISTKYRYDMLAATML 149
           +L +  + + KD+ NAV+AA  A   W     + R+ I    A+ +S +       AT +
Sbjct: 534 LLGHAPEGSRKDIRNAVEAATGAAS-WGRTTGHLRAQILYYVAENLSARTEE---FATRI 589

Query: 150 GQ--GKNVYQAEIDCITELSDFFRYYVKYASDLYAQQPVESADGTWNKAEYRPLEGFVYA 207
            +  G  V +AE +    +   F Y         A +PV          E  P+ G + A
Sbjct: 590 AEMTGSRVPRAEAEVSAAIDRLFTYAAWADKYDGAVKPVPIRGVALAMPE--PV-GVIGA 646

Query: 208 VSPFNFTAIAA-NLIGAPALMGNTVVWKPSQTAALSNYLLMTVLEEAGLPKGVINFIPGD 266
            +P +   + A +++GA   MGN +V  PS+   L+   L  VL+ + +P GV+N + G 
Sbjct: 647 FAPDDAPLLGAVSILGAALAMGNRLVLVPSEPFPLAATDLYQVLDTSDVPAGVVNIVTGA 706

Query: 267 PVQVTDQVLADKDFGAL-HFTG---STNVFKSLYGKI-QSGVVEGKYRDYPRI 314
              +   + +     A+  F+G   ST +     G + ++ V  G+ RD+ ++
Sbjct: 707 HEALAKPLASHLGVDAVWSFSGADISTTIEAEAAGNLKRTWVNHGRARDWMQV 759


Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 962
Number of extensions: 37
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 3
Length of query: 575
Length of database: 780
Length adjustment: 39
Effective length of query: 536
Effective length of database: 741
Effective search space:   397176
Effective search space used:   397176
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources