GapMind for catabolism of small carbon sources

 

Alignments for a candidate for bgtA in Dinoroseobacter shibae DFL-12

Align ATPase (characterized, see rationale)
to candidate 3607933 Dshi_1341 ABC transporter related (RefSeq)

Query= uniprot:Q31RN8
         (261 letters)



>FitnessBrowser__Dino:3607933
          Length = 362

 Score =  152 bits (383), Expect = 1e-41
 Identities = 88/224 (39%), Positives = 131/224 (58%), Gaps = 11/224 (4%)

Query: 41  VSLTVQRGEVVVMMGPSGSGKSTFLRTLNALESHQRGEIWIEGHRLSHDRRDIATIRQEV 100
           VS+ V  G+V  ++GPSG GKST LR +  ++    G + ++G  +S D   +    + V
Sbjct: 27  VSIRVMPGQVTCLLGPSGCGKSTTLRIIAGVDRQDSGTLTVDGEVVSSDDIHLPPEARSV 86

Query: 101 GMVFQQFNLFPHLTVLQNLMLAPVQVRRWPVAQAEATARQLLERVRIAEQADKYPGQLSG 160
           G++FQ F LFPHL V  N+       R+    +  A A +LL+RV +   A K+P QLSG
Sbjct: 87  GLMFQDFALFPHLCVADNVGFGLSGSRK----EKRARAHELLDRVGLLGDAGKFPHQLSG 142

Query: 161 GQQQRVAIARALAMQPRILLFDEPTSALDPEM---VR-EVLDVMRDLASEGMTMLVATHE 216
           G+QQRVA+ARA+A +PR++L DEP S LD  +   +R E L+V++D   EG  +L+ THE
Sbjct: 143 GEQQRVALARAIAPRPRVMLMDEPFSGLDNRLRDGIRDETLEVLKD---EGTAVLLVTHE 199

Query: 217 VGFAREVADRVVLMADGQIVEEAPPDRFFTAPQSDRAKQFLAQI 260
              A  +AD + LM  G+IV++  P   + +P    A  F + I
Sbjct: 200 PEEAMRMADNIALMRGGKIVQQGAPYNVYNSPVDKAAAAFFSDI 243


Lambda     K      H
   0.321    0.134    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 259
Number of extensions: 13
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 261
Length of database: 362
Length adjustment: 27
Effective length of query: 234
Effective length of database: 335
Effective search space:    78390
Effective search space used:    78390
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory