Alignments for a candidate for tctA in Dinoroseobacter shibae DFL-12

GapMind for catabolism of small carbon sources

Alignments for a candidate for tctA in Dinoroseobacter shibae DFL-12

Align Tricarboxylic transport TctA (characterized, see rationale)
to candidate 3610834 Dshi_4220 protein of unknown function DUF112 transmembrane (RefSeq)

Query= uniprot:E4PJQ9
         (508 letters)



>FitnessBrowser__Dino:3610834
          Length = 494

 Score =  332 bits (851), Expect = 2e-95
 Identities = 184/491 (37%), Positives = 282/491 (57%), Gaps = 18/491 (3%)

Query: 7   LMDGFAVALTPYNLMFALFGAFVGTLIGCLPGLGPANGVAILIPLAFTLGLPPETAMILL 66
           L   FA+ L+P  L+    G  +G ++G LPG+G    VA+++P  FTL +    A++LL
Sbjct: 5   LPQAFALLLSPQGLLVLSVGTMLGIVLGALPGIGSTVAVAMILP--FTLTMDQAPAILLL 62

Query: 67  TAVYAGAMYGGRISSILLNIPGDEPAMMTCLDGYPMAQKGRAADALAVSAIASFAGGLIG 126
            A+YAG++YGG IS+IL+N PG   +  TCLDG+PMAQ+G A  AL  + IAS  GGL  
Sbjct: 63  LAIYAGSVYGGSISAILINTPGTPQSAATCLDGFPMAQRGEAGKALGWATIASVVGGLTS 122

Query: 127 TIGLIMLAPVLAKFALTFGPAEYFALFLLAFATLGGITGKNPVKTVVAATLGIMISTVGI 186
            + LI  AP LA FAL FGP E FAL LL    +  ++  + VK ++A  LGI +STVG 
Sbjct: 123 AVVLIFAAPQLAAFALNFGPIETFALILLGLTCIVSVSEGSLVKGLMAGMLGIFLSTVGG 182

Query: 187 DISTGTQRYTFGVLELYEGIDFILAIVGLFAISELLFFVESRMGRGRDKMNVGKLTLTMK 246
           D  TG  R+TFG  +L  G + +  ++G+FA+SE+L     R   G D   V    +  K
Sbjct: 183 DPITGEARFTFGQFQLIAGFNLLAVVIGVFALSEVLI----RASSGPDSTGV---LVDFK 235

Query: 247 ELVMTIPTQLRGGVLGFIS--------GVLPGAGASLGSFISYTLEKQVVGKKGKFGEGD 298
            +V+    + +G + G           G+LPG GA+  +FISY   ++    +  FG+G+
Sbjct: 236 GIVLPRWAEWKGRLRGLAKSVAIGNGVGILPGTGAATAAFISYAEARRSAPTRANFGKGE 295

Query: 299 IRGVVAPEAGNNGASSGALVPMLTLGVPGSGTTAVLLAMLISLNITPGPLMFTQNADIVW 358
             G++A E+ NN  + GALVP + LG+PG   TAV+LA L    +TPG  +   N  ++ 
Sbjct: 296 PDGLIASESANNAVTGGALVPTMALGIPGDAITAVMLATLTLHGVTPGIRLMQDNPVLMA 355

Query: 359 GVIAALLIGNVLLLVLNIPLVGFFVKLLSVPPMYLLPIVTMVAFVGIYSISHSTFDLYFM 418
            + A   I N++LL L + +      LL +   Y+L ++T++  VG++ +  + FDL  M
Sbjct: 356 SIFAGFFIINLMLLPLGMLVSKLAAPLLRMREAYMLIVITLLCTVGVFFVRGNPFDLLVM 415

Query: 419 VAFGVAGYFLRKLEIPLVPIILGLLLGPEMEKNLGHALVLSDGEWSVLWAS-PLAMGLWI 477
              G+ G+ LR+   P+ P+++G++LGP +E +L   L+++DG +   +   P+A+GL I
Sbjct: 416 AGAGIVGFVLRRQGYPMAPLVIGMVLGPTLELSLRQGLIITDGNFGAFFTGHPIALGLTI 475

Query: 478 VAGLGLILPYL 488
            A   L LP +
Sbjct: 476 AAAGMLSLPLI 486


Lambda     K      H
   0.325    0.144    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 779
Number of extensions: 50
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 508
Length of database: 494
Length adjustment: 34
Effective length of query: 474
Effective length of database: 460
Effective search space:   218040
Effective search space used:   218040
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources