GapMind for catabolism of small carbon sources

 

2-deoxy-D-ribose catabolism in Dinoroseobacter shibae DFL-12

Best path

deoP, deoK, deoC, adh, ackA, pta

Also see fitness data for the top candidates

Rules

Overview: Deoxyribose utilization in GapMind is based on MetaCyc pathways 2-deoxy-D-ribose degradation I via deoxyribose 5-phosphate aldolase (link) and pathway II via oxidation to 2-deoxy-3-dehydro-D-ribonate (link).

19 steps (16 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
deoP deoxyribose transporter
deoK deoxyribokinase Dshi_1976 Dshi_1621
deoC deoxyribose-5-phosphate aldolase Dshi_3016
adh acetaldehyde dehydrogenase (not acylating) Dshi_3017 Dshi_1095
ackA acetate kinase Dshi_0632 Dshi_5006
pta phosphate acetyltransferase Dshi_1825 Dshi_0633
Alternative steps:
aacS acetoacetyl-CoA synthetase Dshi_3403 Dshi_0833
acs acetyl-CoA synthetase, AMP-forming Dshi_3553 Dshi_1399
ald-dh-CoA acetaldehyde dehydrogenase, acylating
atoA acetoacetyl-CoA transferase, A subunit Dshi_1136
atoB acetyl-CoA C-acetyltransferase Dshi_3066 Dshi_3331
atoD acetoacetyl-CoA transferase, B subunit Dshi_1134
deoxyribonate-dehyd 2-deoxy-D-ribonate 3-dehydrogenase Dshi_2891 Dshi_0620
deoxyribonate-transport 2-deoxy-D-ribonate transporter
drdehyd-alpha 2-deoxy-D-ribose dehydrogenase, alpha subunit Dshi_0864 Dshi_4201
drdehyd-beta 2-deoxy-D-ribose dehydrogenase, beta subunit Dshi_0865
drdehyd-cytc 2-deoxyribose-D dehydrogenase, cytochrome c component Dshi_4060 Dshi_3784
garK glycerate 2-kinase Dshi_3046
ketodeoxyribonate-cleavage 2-deoxy-3-keto-D-ribonate cleavage enzyme Dshi_3475

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory