Alignments for a candidate for deoC in Dinoroseobacter shibae DFL-12

GapMind for catabolism of small carbon sources

Alignments for a candidate for deoC in Dinoroseobacter shibae DFL-12

Align deoxyribose-phosphate aldolase (EC 4.1.2.4) (characterized)
to candidate 3609633 Dshi_3016 deoxyribose-phosphate aldolase (RefSeq)

Query= BRENDA::Q9Y315
         (318 letters)



>FitnessBrowser__Dino:3609633
          Length = 339

 Score =  368 bits (945), Expect = e-106
 Identities = 189/309 (61%), Positives = 235/309 (76%), Gaps = 4/309 (1%)

Query: 5   NRGTELDLSWISKIQVNHPAVLRRAEQIQARRTVKKEWQAAWLLKAVTFIDLTTLSGDDT 64
           N G  LDL W+    VN  AV RRA  +  RR+VKK+ QAAWLLKAVT IDLTTL+GDDT
Sbjct: 30  NPGLPLDLDWVRSAAVNTSAVERRAASLPGRRSVKKDHQAAWLLKAVTLIDLTTLAGDDT 89

Query: 65  SSNIQRLCYKAKYPIREDLLKALNMHDKGITTAAVCVYPARVCDAVKALKAAGCNIPVAS 124
           +  ++RLC KA+ P+  ++L AL M    +TT AVCVY   V  AV+AL+ +G  IPVA+
Sbjct: 90  AGRVRRLCAKARQPVAPEVLAALGMGP--VTTGAVCVYHDMVHVAVEALEGSG--IPVAA 145

Query: 125 VAAGFPAGQTHLKTRLEEIRLAVEDGATEIDVVINRSLVLTGQWEALYDEIRQFRKACGE 184
           V+ GFPAG +    R+ EI  +V  GA EID+VI+R  VLTG W+ALYDE+R FR ACG+
Sbjct: 146 VSTGFPAGLSPFHLRVAEIEESVAAGAAEIDIVISRRHVLTGNWQALYDEMRAFRAACGD 205

Query: 185 AHLKTILATGELGTLTNVYKASMIAMMAGSDFIKTSTGKETVNATFPVAIVMLRAIRDFF 244
           AH+K ILATGELG L NV +AS++ MMAG+DFIKTSTGKE+VNAT PV++VM+RAIRD+ 
Sbjct: 206 AHVKAILATGELGDLGNVARASLVCMMAGADFIKTSTGKESVNATLPVSLVMIRAIRDYE 265

Query: 245 WKTGNKIGFKPAGGIRSAKDSLAWLSLVKEELGDEWLKPELFRIGASTLLSDIERQIYHH 304
             TG K+G+KPAGGI  AKD+L +LSL+KEELGD WL+P+LFR GAS+LL DIERQ+ HH
Sbjct: 266 AATGVKVGYKPAGGISKAKDALVYLSLIKEELGDRWLQPDLFRFGASSLLGDIERQLEHH 325

Query: 305 VTGRYAAYH 313
           VTG Y+A H
Sbjct: 326 VTGAYSATH 334


Lambda     K      H
   0.320    0.133    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 367
Number of extensions: 13
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 318
Length of database: 339
Length adjustment: 28
Effective length of query: 290
Effective length of database: 311
Effective search space:    90190
Effective search space used:    90190
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate 3609633 Dshi_3016 (deoxyribose-phosphate aldolase (RefSeq))
to HMM TIGR00126 (deoC: deoxyribose-phosphate aldolase (EC 4.1.2.4))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00126.hmm
# target sequence database:        /tmp/gapView.2057.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00126  [M=211]
Accession:   TIGR00126
Description: deoC: deoxyribose-phosphate aldolase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                         Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                         -----------
    1.3e-48  151.3   0.3    2.6e-48  150.3   0.3    1.4  1  lcl|FitnessBrowser__Dino:3609633  Dshi_3016 deoxyribose-phosphate 


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Dino:3609633  Dshi_3016 deoxyribose-phosphate aldolase (RefSeq)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  150.3   0.3   2.6e-48   2.6e-48       3     203 ..      76     299 ..      74     303 .. 0.94

  Alignments for each domain:
  == domain 1  score: 150.3 bits;  conditional E-value: 2.6e-48
                         TIGR00126   3 akliDhtalkadtteedietlcaeAkky..............kfaavcvnpsyvslAkelLkgteveictv.vgFPl 64 
                                        +liD+t+l  d+t   + +lca+A ++               + avcv+  +v++A+e L+g+ + +++v +gFP+
  lcl|FitnessBrowser__Dino:3609633  76 VTLIDLTTLAGDDTAGRVRRLCAKARQPvapevlaalgmgpvTTGAVCVYHDMVHVAVEALEGSGIPVAAVsTGFPA 152
                                       689**********************998888888878777777889************************978**** PP

                         TIGR00126  65 GasttevkllEakeaieeGAdEvDvviniaalkdkneevviedikavveacakvllKvilEtalLtdeekk.kAsei 140
                                       G s  + +++E +e++++GA E+D+vi  + +  +n+++ +++++a   ac+++++K+il t++L d   + +As +
  lcl|FitnessBrowser__Dino:3609633 153 GLSPFHLRVAEIEESVAAGAAEIDIVISRRHVLTGNWQALYDEMRAFRAACGDAHVKAILATGELGDLGNVaRASLV 229
                                       *******************************************************************87666***** PP

                         TIGR00126 141 sieagadfvKtstgfsakgAtvedvrlmkkvvgd.......evgvKasGGvrtaedalalieagaeriga 203
                                       ++ agadf+Ktstg+  ++At+    +m ++++d       +vg K++GG+  a+dal +++   e +g 
  lcl|FitnessBrowser__Dino:3609633 230 CMMAGADFIKTSTGKESVNATLPVSLVMIRAIRDyeaatgvKVGYKPAGGISKAKDALVYLSLIKEELGD 299
                                       ***********************999**********************************9988888875 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (211 nodes)
Target sequences:                          1  (339 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 3.89
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources