Alignments for a candidate for adhAqn in Dinoroseobacter shibae DFL-12

GapMind for catabolism of small carbon sources

Alignments for a candidate for adhAqn in Dinoroseobacter shibae DFL-12

Align alcohol dehydrogenase (quinone) (EC 1.1.5.5) (characterized)
to candidate 3607055 Dshi_0476 Pyrrolo-quinoline quinone (RefSeq)

Query= BRENDA::P18278
         (742 letters)



>FitnessBrowser__Dino:3607055
          Length = 600

 Score =  292 bits (748), Expect = 3e-83
 Identities = 208/635 (32%), Positives = 296/635 (46%), Gaps = 68/635 (10%)

Query: 15  ILAAG-TICAAALPYAAVPARADGQGNTGEAIIHADDHPENWLSYGRTYSEQRYSPLDQI 73
           +L +G  +CAA + YA             + +I   D  + W      Y   RYS LD+I
Sbjct: 6   LLTSGIALCAATVAYA------------NDDLITQMDDSKQWAIQTGDYKNHRYSELDEI 53

Query: 74  NRSNVGDLKLLGYYTLDTNRGQEATPLVVDGIMYATTNWSKME-ALDAAT-GKLLWQYDP 131
           N  NVGDL++   ++    RG E +PLV+   MY  T +  +  ALD +  GK++W+Y+P
Sbjct: 54  NAENVGDLQVAWTFSTGVLRGHEGSPLVIGDTMYVHTPFPNIVYALDLSNEGKIIWKYEP 113

Query: 132 KVPGNIADKGCCDTVNRGAGYWNGKVFWGTFDGRLVAADAKTGKKVWAVNTIPADASLGK 191
           K   ++    CCDTVNRG  Y +GK+F    D ++VA DA TG  +W V     D S+G+
Sbjct: 114 KQDPDVIPVMCCDTVNRGVAYADGKIFLHQADTKVVALDANTGDVLWEVQN--GDPSIGE 171

Query: 192 QRSYTVDGAVRVAKGLVLIGNGGAEFGARGFVSAFDAETGKLKWRFYTVPNNKN---EPD 248
             + TV       K  V++G  G EFG RG V+A+D  +G+L WR Y++  + +   +P+
Sbjct: 172 TNTATV----LPVKDKVIVGISGGEFGVRGHVTAYDINSGELVWRAYSLGPDDDMLVDPE 227

Query: 249 HAA--SDNILMNKAYKTWGPKGAWVRQGGGGTVWDSLVYDPVSDLIYLAVGNGSPWNYKY 306
           +       I  N +  +W     W+   GGGT W     D   +L Y   GN S WN   
Sbjct: 228 NTTHLGKPIGENSSLNSW-EGDQWMI--GGGTTWGWYAADMEENLFYYGTGNPSTWNPAQ 284

Query: 307 RSEGIGSNLFLGSIVALKPETGEYVWHFQATPMDQWDYTSVQQIMTLDMPVKGEMRHVIV 366
           R    G N +  +++A   +TG   W +Q TP D+WDY  V + +  +    GEMR ++ 
Sbjct: 285 RP---GDNRWTMTVMARDIDTGMAKWFYQMTPHDEWDYDGVNENILTEQEFDGEMRKLLT 341

Query: 367 HAPKNGFFYVLDAKTGEFLSGKNY-VYQNWANGLD-----PLTGRPMYNPDGLYTLNGKF 420
              +NG  Y +D  TGE L  + +    NW  G+D        GRP          NG+ 
Sbjct: 342 RFDRNGLAYTMDRVTGELLVAEKFDPVVNWTTGVDMDPNSETYGRPQVVAQYSTAQNGED 401

Query: 421 --WYGI-PGPLGAHNFMAMAYSPKTHLVYIPAHQIPFGYKNQVGGFKPHADSWNVGLDMT 477
               GI P  LG  +    AYSPKT + Y+P + +   Y+       P   S+  G    
Sbjct: 402 VNTTGICPAALGTKDQQPAAYSPKTQVFYVPTNHVCMDYE-------PFRVSYTAGQPYV 454

Query: 478 KNGLPDTPEARTAYIKDLHGWL---LAWDPVKMETVWKIDHKGPWNGGILATGGDLLFQG 534
              L   P        D HG +   +AWD +  E +W I  K     G LAT GD++F G
Sbjct: 455 GATLSMYPAP------DSHGGMGNFIAWDNINGEILWSIPEKFSVWSGALATAGDVVFYG 508

Query: 535 LANGEFHAYDATNGSDLYKFDAQSGIIAPPMTYSVNGKQYVAVEVGWGGIYPISMG---- 590
              G   A  A  G +LY+F   SGII   MTY  +G+QYV V  G GG   I +     
Sbjct: 509 TLEGHLKAVHADTGEELYRFKTPSGIIGNVMTYEHDGRQYVGVLSGVGGWAGIGLAAGLT 568

Query: 591 ----GVGRTSGWTVNHSYIAAFSLDGKAKLPALNN 621
               G+G   G+     Y A   L G+  + AL N
Sbjct: 569 NPNDGLGAVGGYAALSDYTA---LGGQLTVFALPN 600


Lambda     K      H
   0.318    0.137    0.439 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1642
Number of extensions: 125
Number of successful extensions: 11
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 742
Length of database: 600
Length adjustment: 38
Effective length of query: 704
Effective length of database: 562
Effective search space:   395648
Effective search space used:   395648
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources