Alignments for a candidate for etoh-dh-c in Dinoroseobacter shibae DFL-12

GapMind for catabolism of small carbon sources

Alignments for a candidate for etoh-dh-c in Dinoroseobacter shibae DFL-12

Align quinoprotein decanol dehydrogenase; EC 1.1.-.- (characterized)
to candidate 3609287 Dshi_2673 Pyrrolo-quinoline quinone (RefSeq)

Query= CharProtDB::CH_107432
         (595 letters)



>FitnessBrowser__Dino:3609287
          Length = 588

 Score =  767 bits (1981), Expect = 0.0
 Identities = 352/588 (59%), Positives = 452/588 (76%), Gaps = 21/588 (3%)

Query: 12  VSLVLSAMLLAGAAHA-AVSNEEILQDPKNPQQIVTNGLGVQGQRYSPLDLLNANNVKEL 70
           ++ V +A++  GAA+A  ++ +++  D  +  +++TNG+G   QRYSPL+ LN +NV+ L
Sbjct: 5   IAAVTAAVVATGAAYAEGITEQDLANDQADTSRVLTNGMGRHLQRYSPLETLNKDNVENL 64

Query: 71  RPVWAFSFGGEKQRGQQAQPLIKDGVMYLTGSYSRVFAVDARTGKKLWQYDARLPDDIRP 130
            P WAFS GGEKQRGQ+ QPLI DG+MY+TGSYSRV+A+D  TGK++WQYDARLP+ I P
Sbjct: 65  VPAWAFSLGGEKQRGQETQPLIYDGIMYITGSYSRVYAIDVETGKEIWQYDARLPEGILP 124

Query: 131 CCDVINRGVALYGDLVFFGTLDAKLVALNKDTGKVVWSKKVADHKEGYSISAAPMIVNGK 190
           CCDVINRG A+YGD  +FGTLDA++VAL+  TG VVW KK+ D+K GYS +AAP+IVNG 
Sbjct: 125 CCDVINRGGAIYGDKFYFGTLDARIVALDLKTGDVVWRKKIDDYKAGYSYTAAPLIVNGL 184

Query: 191 LITGVAGGEFGVVGKIQAYNPENGELLWMRPTVEGHMGYVYKDGKAIENGISGGEAGKTW 250
           ++TG +GGEFG+VG ++A + E GEL+W RP +EGHMG +  +   +      G    TW
Sbjct: 185 VVTGNSGGEFGIVGAVEARDAETGELVWKRPVIEGHMGELNGEPSTMT-----GTLNATW 239

Query: 251 PGDLWKTGGAAPWLGGYYDPETNLILFGTGNPAPWNSHLRP---------GDNLYSSSRL 301
           PGD+WKTGG A WLGG YD  T+ ++FGTGNPAPWNSHLR          GDNLY++SRL
Sbjct: 240 PGDMWKTGGGATWLGGSYDARTDTLIFGTGNPAPWNSHLRGAGKPSEDGMGDNLYAASRL 299

Query: 302 ALNPDDGTIKWHFQSTPHDGWDFDGVNELISFNYKDGGKEVKAAATADRNGFFYVLDRTN 361
            ++P  G IKWHFQ+TP +GWDFDGVNE+++++ ++G   +   ATADRNGFFYVLD  +
Sbjct: 300 GIDPATGEIKWHFQTTPREGWDFDGVNEVVAYDDREGNPRL---ATADRNGFFYVLDAAD 356

Query: 362 GKFIRGFPFVDKITWATGLDKDGRPIYNDASRPGAPGSEA---KGSSVFVAPAFLGAKNW 418
           G F+ G PFV++I+WA+GLD++GRPI+ + +RPG P   A   KG +VF  P FLG KNW
Sbjct: 357 GSFVSGMPFVEQISWASGLDENGRPIFVEENRPGDPAEAADGKKGETVFAVPGFLGGKNW 416

Query: 419 MPMAYNKDTGLFYVPSNEWGMDIWNEGIAYKKGAAFLGAGFTIKPLNEDYIGVLRAIDPI 478
           MPMAY++ TG FYVP+NEWGMDIWNE I YKKGAA+LG+GFTIKPL ED+IG ++AIDP 
Sbjct: 417 MPMAYSQRTGNFYVPANEWGMDIWNEPITYKKGAAYLGSGFTIKPLFEDHIGAIKAIDPD 476

Query: 479 SGKEVWRHKNYAPLWGGVLTTKGNLVFTGTPEGFLQAFNAKTGDKVWEFQTGSGVLGSPV 538
           + +  W +KN APLW GV+TT G LVF GTPEG   A + +TG+K+W FQTGSG++G P+
Sbjct: 477 TMETKWVYKNDAPLWSGVMTTAGGLVFFGTPEGEFIALDDETGEKLWSFQTGSGIVGQPI 536

Query: 539 TWEMDGEQYVSVVSGWGGAVPLWGGEVAKRVKDFNQGGMLWTFKLPKQ 586
           TWE DGEQYVSV+SGWGGAVPLWGGEVAK+V   NQGGMLWTF+LPKQ
Sbjct: 537 TWEQDGEQYVSVISGWGGAVPLWGGEVAKKVNYLNQGGMLWTFRLPKQ 584


Lambda     K      H
   0.317    0.137    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1732
Number of extensions: 135
Number of successful extensions: 16
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 595
Length of database: 588
Length adjustment: 37
Effective length of query: 558
Effective length of database: 551
Effective search space:   307458
Effective search space used:   307458
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources