Alignments for a candidate for etoh-dh-nad in Dinoroseobacter shibae DFL-12

GapMind for catabolism of small carbon sources

Alignments for a candidate for etoh-dh-nad in Dinoroseobacter shibae DFL-12

Align Alcohol dehydrogenase class-3; EC 1.1.1.1; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; EC 1.1.1.-; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.284 (uncharacterized)
to candidate 3606672 Dshi_0103 Alcohol dehydrogenase class III/S-(hydroxymethyl)glutathione dehydrogenase (RefSeq)

Query= curated2:P72324
         (376 letters)



>FitnessBrowser__Dino:3606672
          Length = 370

 Score =  641 bits (1653), Expect = 0.0
 Identities = 307/376 (81%), Positives = 344/376 (91%), Gaps = 6/376 (1%)

Query: 1   MRTRAAVAVEAGKPLEIMEVNLEGPKAGEVMVEIKATGICHTDEFTLSGADPEGMFPAIL 60
           MRT+AAVA+ AGKPLE+MEVNLEGPKAGEV+VEIKATGICHTDEFTLSGADPEG+FPAIL
Sbjct: 1   MRTKAAVAIAAGKPLEVMEVNLEGPKAGEVLVEIKATGICHTDEFTLSGADPEGLFPAIL 60

Query: 61  GHEGAGVVVEVGPGVTSVKPGDHVIPLYTPECRQCPSCLSQKTNLCTAIRGTQGQGLMPD 120
           GHEGAGVV+EVGPGVTS+KPGDHVIPLYTPECR+C  CL+ KTNLC +IR TQG GLMPD
Sbjct: 61  GHEGAGVVMEVGPGVTSLKPGDHVIPLYTPECRECEYCLNPKTNLCQSIRTTQGAGLMPD 120

Query: 121 GTSRFSMLDGTPILHYMGCSTFSNYTVLPEIAVAKVRPDAPFDKICYIGCGVTTGIGAVI 180
           GTSRFS LDG PILHYMGCSTF+N+TVLPEIA+AKVRPDAPFDKICYIGCGVTTGIGAVI
Sbjct: 121 GTSRFSTLDGDPILHYMGCSTFANHTVLPEIALAKVRPDAPFDKICYIGCGVTTGIGAVI 180

Query: 181 NTAKVEIGAKAVVFGLGGIGLNVIQGLKLAGADMIIGVDLNNAKKEWGERFGMTHFVNPS 240
           NTAKVEIG++A+VFGLGGIGLNVIQGL+LAGAD I+GVDLN  K    ERFGMT FVNP+
Sbjct: 181 NTAKVEIGSRAIVFGLGGIGLNVIQGLRLAGADQIVGVDLNPDKIPMAERFGMTDFVNPA 240

Query: 241 EIDGDVVAHLVNMTKTPFDQIGGADYTFDCTGNVKVMRQALEACHRGWGQSIVIGVAPAG 300
           E++GD+V +LV++TK      GGADYTFD TGNV+VMR ALE+ H+GWG+SI+IGVAPAG
Sbjct: 241 EVEGDLVPYLVDLTK------GGADYTFDATGNVQVMRAALESAHKGWGESIIIGVAPAG 294

Query: 301 AEIQTRPFQLVTGRVWKGSAFGGARGRTDVPKIVDWYMEGKIQIDPMITHILSLEEINKG 360
           AEI TRPFQLVTGRVW+G+AFGGARGRTDVPKIVDWYMEGKI+IDPMITH + L +IN G
Sbjct: 295 AEIATRPFQLVTGRVWRGTAFGGARGRTDVPKIVDWYMEGKIEIDPMITHTMPLSDINTG 354

Query: 361 FDLMHAGESIRSVVVF 376
           FDLMHAGESIRSVV++
Sbjct: 355 FDLMHAGESIRSVVIY 370


Lambda     K      H
   0.320    0.139    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 615
Number of extensions: 17
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 376
Length of database: 370
Length adjustment: 30
Effective length of query: 346
Effective length of database: 340
Effective search space:   117640
Effective search space used:   117640
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources