Alignments for a candidate for HSERO_RS05250 in Dinoroseobacter shibae DFL-12

GapMind for catabolism of small carbon sources

Alignments for a candidate for HSERO_RS05250 in Dinoroseobacter shibae DFL-12

Align Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale)
to candidate 3607108 Dshi_0530 ABC transporter related (RefSeq)

Query= uniprot:D8J111
         (520 letters)



>FitnessBrowser__Dino:3607108
          Length = 498

 Score =  326 bits (836), Expect = 1e-93
 Identities = 182/495 (36%), Positives = 288/495 (58%), Gaps = 6/495 (1%)

Query: 15  SSSSSVPVIALRNVCKRFPGVLALDNCQFELAAGEVHALMGENGAGKSTLMKILSGVYQR 74
           SS      I LR + KR+ GV ALD+  F +  GE   L GENG+GKSTL+KI+SGV   
Sbjct: 2   SSDHDTAFIDLRAITKRYAGVTALDSVDFTVQPGEAVCLAGENGSGKSTLIKIISGVEPA 61

Query: 75  DSGDILLDGKPVEITEPRQAQALGIGIIHQELNLMNHLSAAQNIFIGREPRKAMGLFIDE 134
            +G + + G+      PR + A G+ +I Q+ +L  +LS A+NI    +      LF   
Sbjct: 62  TAGTVQIAGQEHVTLNPRISAAAGVMVIFQDFSLFPNLSVAENIAFTTQLSTRQRLFKFR 121

Query: 135 DELNRQAAAIFARMRLDMDPSTPVGELTVARQQMVEIAKALSFDSRVLIMDEPTAALNNA 194
             +   A A   R+ + +D    V  L VA++Q+V I +AL+  ++++IMDEPT AL   
Sbjct: 122 -AVRDIARAALDRIGVQIDLDARVETLPVAQKQLVAICRALASKAQLIIMDEPTTALTEK 180

Query: 195 EIAELFRIIRDLQAQGVGIVYISHKMDELRQIADRVSVMRDGKYIATVPMQETSMDTIIS 254
           E+  L  IIR L+ +GV ++++SHK+ E+ +++++V V+R+GK +A  P  E    ++  
Sbjct: 181 EVRRLQGIIRMLKEEGVAVIFVSHKLAEVLEVSEKVVVLRNGKKVAEGPASEFDTQSLTY 240

Query: 255 MMVGRALDGEQRIPPD--TSRNDVVLEVRGLNRGRAIRDVSFTLRKGEILGFAGLMGAGR 312
            M GR +     +PP    +    +++V+GL +  +  D+SF LR GE+LG  GL+G GR
Sbjct: 241 HMTGRDVP---EVPPSDVAAGAQTLMQVQGLGKAGSFSDISFDLRTGEVLGITGLLGCGR 297

Query: 313 TEVARAIFGADPLEAGEIIIHGGKAVIKSPADAVAHGIGYLSEDRKHFGLAVGMDVQANI 372
           T VA+A+FG    +AG I++ G    +  P  A    IGY+ EDR   GL +   +  N+
Sbjct: 298 TSVAKALFGLVTPDAGSILVDGSPVPLGDPQAASLARIGYVPEDRLTEGLFLSQSILRNV 357

Query: 373 ALSSMGRFTRVGFMDQRAIREAAQMYVRQLAIKTPSVEQQARLLSGGNQQKIVIAKWLLR 432
           A+  +   T  GF+D   + + A  ++R+L +K P VE   + LSGGNQQ++ +A+WL R
Sbjct: 358 AVGRLDAHTSGGFLDMTGLAKEASDWLRRLKVKAPDVEAPVQSLSGGNQQRVALARWLSR 417

Query: 433 DCDILFFDEPTRGIDVGAKSEIYKLLDALAEQGKAIVMISSELPEVLRMSHRVLVMCEGR 492
              +L  + P+ G+DVG+K++I+ ++  LA +G  +++IS +LPE+L   HRVLVM EGR
Sbjct: 418 APRVLILNGPSVGVDVGSKADIHDIIRELAREGIGVIVISDDLPELLATCHRVLVMREGR 477

Query: 493 ITGELARADATQEKI 507
           I   L     T++ +
Sbjct: 478 IIDALEGTALTEDDL 492



 Score = 87.4 bits (215), Expect = 1e-21
 Identities = 58/228 (25%), Positives = 113/228 (49%), Gaps = 5/228 (2%)

Query: 288 AIRDVSFTLRKGEILGFAGLMGAGRTEVARAIFGADPLEAGEIIIHGGKAVIKSPADAVA 347
           A+  V FT++ GE +  AG  G+G++ + + I G +P  AG + I G + V  +P  + A
Sbjct: 24  ALDSVDFTVQPGEAVCLAGENGSGKSTLIKIISGVEPATAGTVQIAGQEHVTLNPRISAA 83

Query: 348 HGIGYLSEDRKHFGLAVGMDVQANIALSSMGRFTRVGFMDQRAIREAAQMYVRQLAIKTP 407
            G+  + +D   F L   + V  NIA ++    TR      RA+R+ A+  + ++ ++  
Sbjct: 84  AGVMVIFQD---FSLFPNLSVAENIAFTTQ-LSTRQRLFKFRAVRDIARAALDRIGVQI- 138

Query: 408 SVEQQARLLSGGNQQKIVIAKWLLRDCDILFFDEPTRGIDVGAKSEIYKLLDALAEQGKA 467
            ++ +   L    +Q + I + L     ++  DEPT  +       +  ++  L E+G A
Sbjct: 139 DLDARVETLPVAQKQLVAICRALASKAQLIIMDEPTTALTEKEVRRLQGIIRMLKEEGVA 198

Query: 468 IVMISSELPEVLRMSHRVLVMCEGRITGELARADATQEKIMQLATQRE 515
           ++ +S +L EVL +S +V+V+  G+   E   ++   + +    T R+
Sbjct: 199 VIFVSHKLAEVLEVSEKVVVLRNGKKVAEGPASEFDTQSLTYHMTGRD 246


Lambda     K      H
   0.320    0.135    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 562
Number of extensions: 34
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 520
Length of database: 498
Length adjustment: 34
Effective length of query: 486
Effective length of database: 464
Effective search space:   225504
Effective search space used:   225504
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources