Align L-arabinonate dehydratase; ArDHT; D-fuconate dehydratase; Galactonate dehydratase; L-arabonate dehydratase; EC 4.2.1.25; EC 4.2.1.67; EC 4.2.1.6 (characterized)
to candidate 3607836 Dshi_1244 Dihydroxy-acid dehydratase (RefSeq)
Query= SwissProt::B5ZZ34 (579 letters) >FitnessBrowser__Dino:3607836 Length = 569 Score = 659 bits (1701), Expect = 0.0 Identities = 334/568 (58%), Positives = 420/568 (73%), Gaps = 4/568 (0%) Query: 12 RSQEWYGGTSRDVIYHRGWLKNQGYPHDLFDGRPVIGILNTWSDMTPCNGHLRELAEKVK 71 R++ WYG +D HR W+KNQG+P +FDGRPVIGI NTWS++TPCN LR LAE VK Sbjct: 6 RNRAWYGKLDKDGFIHRSWMKNQGFPDHVFDGRPVIGICNTWSELTPCNHGLRTLAEGVK 65 Query: 72 AGVWEAGGFPLEVPVFSASENTFRPTAMMYRNLAALAVEEAIRGQPMDGCVLLVGCDKTT 131 GVWEAGGFP+E PV S E +PTAM++RNL A+ VEE+IR MDG VLL GCDKTT Sbjct: 66 RGVWEAGGFPVEFPVMSLGETQMKPTAMLFRNLLAMDVEESIRAYGMDGVVLLGGCDKTT 125 Query: 132 PSLLMGAASCDLPSIVVTGGPMLNGYFRGERVGSGTHLWKFSEMVKAGEMTQAEFLEAEA 191 P LMGAAS DLPSIVV+ GPMLNG +RG+ +GSGT +WKFSE V+AGEMT A+F+ AEA Sbjct: 126 PGQLMGAASVDLPSIVVSAGPMLNGKYRGQDIGSGTDVWKFSEAVRAGEMTLADFMNAEA 185 Query: 192 SMSRSSGTCNTMGTASTMASMAEALGMALSGNAAIPGVDSRRKVMAQLTGRRIVQMVKDD 251 MSRS+G C TMGTASTMAS+ EA+GM+L NAA+P VD+RR +A LTG+RIV+MV++D Sbjct: 186 GMSRSAGVCMTMGTASTMASLVEAMGMSLPLNAALPAVDARRMALAHLTGKRIVEMVEED 245 Query: 252 LKPSEIMTKQAFENAIRTNAAIGGSTNAVIHLLAIAGRVGIDLSLDDWDRCGRDVPTIVN 311 L+PS I+TK AFENAI NAA+GGSTNAV+HLLA+AGR G+DL+L D++ G ++P +VN Sbjct: 246 LRPSAILTKPAFENAILANAAVGGSTNAVMHLLALAGRAGVDLTLKDFE-IGGEIPLLVN 304 Query: 312 LMPSGKYLMEEFFYAGGLPVVLKRLGEAGLLHKDALTVSGETVWDEVKDVVNWNEDVILP 371 MPSGKYLME+F YAGG+PVVL L + + A TV G+ + + +N DVI Sbjct: 305 CMPSGKYLMEDFAYAGGMPVVLSELRDH---LRPATTVLGKDIAAYTEGAECFNRDVIHA 361 Query: 372 AEKALTSSGGIVVLRGNLAPKGAVLKPSAASPHLLVHKGRAVVFEDIDDYKAKINDDNLD 431 + + + G+ VLRG+LAP GA++KPSAA+ LL H+G A VFE+I+D KA I+ D+L Sbjct: 362 YDAPVKPAAGLRVLRGSLAPDGAIVKPSAATDALLEHEGPAFVFENIEDMKANIDRDDLP 421 Query: 432 IDENCIMVMKNCGPKGYPGMAEVGNMGLPPKVLKKGILDMVRISDARMSGTAYGTVVLHT 491 I + I+V+K CGPKGYPGM EVGNM +P K++++G+ DM+R+SDARMSGTAYGTV+LH Sbjct: 422 ITPDTILVLKGCGPKGYPGMPEVGNMPIPAKLVREGVRDMIRVSDARMSGTAYGTVILHV 481 Query: 492 SPEAAVGGPLAVVKNGDMIELDVPNRRLHLDISDEELARRLAEWQPNHDLPTSGYAFLHQ 551 +PEA GGPLA+VK GD I + L L + ELA R A WQP+ T GYA L+ Sbjct: 482 APEAQAGGPLALVKTGDRIRVSAREGVLDLLVDATELAARRAAWQPDPPHYTRGYAKLYI 541 Query: 552 QHVEGADTGADLDFLKGCRGNAVGKDSH 579 HV AD GADLDFL G V ++SH Sbjct: 542 DHVLQADRGADLDFLVGKDTRPVTRESH 569 Lambda K H 0.318 0.135 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1036 Number of extensions: 49 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 579 Length of database: 569 Length adjustment: 36 Effective length of query: 543 Effective length of database: 533 Effective search space: 289419 Effective search space used: 289419 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory