Alignments for a candidate for ytfR in Dinoroseobacter shibae DFL-12

GapMind for catabolism of small carbon sources

Alignments for a candidate for ytfR in Dinoroseobacter shibae DFL-12

Align galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) (characterized)
to candidate 3609044 Dshi_2433 ABC transporter related (RefSeq)

Query= ecocyc::YTFR-MONOMER
         (500 letters)



>FitnessBrowser__Dino:3609044
          Length = 510

 Score =  334 bits (856), Expect = 5e-96
 Identities = 181/482 (37%), Positives = 291/482 (60%), Gaps = 10/482 (2%)

Query: 7   QEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGENGAGKSTLIKALTGVYHADRGTI 66
           Q  L    ++K FPGVKAL +V  SL  G++ AL+GENGAGKST++K LTG+Y  D G I
Sbjct: 18  QAALALAHITKTFPGVKALSDVSLSLYPGKVTALIGENGAGKSTVVKILTGIYQPDGGRI 77

Query: 67  WLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVADNLFIGREPK-RFGLLRRKEMEKR 125
            ++GQ +       A   G+  ++QE  L   +SVA+N+F+G  P+  FGL+  K+  + 
Sbjct: 78  LVDGQPVPFSTPQAAADHGVTAIHQETVLFDELSVAENIFLGHAPRGAFGLIDWKKTTEN 137

Query: 126 ATELMASYGFSLDVREPLNRFSVAMQQIVAICRAIDLSAKVLILDEPTASLDTQEVELLF 185
           A  L+ S G  LD    L    +A + +VAI RA+ + A+V+I+DEPTA+L  +E+E L+
Sbjct: 138 ARALLTSIGAELDPDHKLKDLGIANKHLVAIARALSIEARVVIMDEPTAALSHKEIEELY 197

Query: 186 DLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVLRNGSFVGCRETCELPQIELVKMMLGRE 245
           +L+  L+ +G +++F++H  D++++++D  TV R+G  +G     ++ + +LVKMM+GR+
Sbjct: 198 ELVESLKAQGKAILFISHKFDEIFRIADNYTVFRDGQLIGDGAIADVTEADLVKMMVGRD 257

Query: 246 LDTHALQRAGRTLLSDKPVAAFKNYGKKGTIAPFDLEVRPGEIVGLAGLLGSGRTETAEV 305
           +     QRA         V   + Y            +R GEI+G  GL+G+GR+E  + 
Sbjct: 258 VSQIFPQRAPNV---GDTVLTVQGYAHPTEFDDISFTLREGEILGFYGLVGAGRSEFMQS 314

Query: 306 IFGIKPADSGTALIKGKPQNLRSPHQASVLGIGFCPEDRKTDGIIAAASVRENII---LA 362
           +FGI    +G+  I G    + SP  A   GI + PEDR   G I    + +N+    L 
Sbjct: 315 LFGITRPSAGSVEIGGARAEISSPADAVDHGIVYVPEDRGKQGAILDLPIFQNVTLPSLG 374

Query: 363 LQAQRGWLRPISRKEQQEIAERFIRQLGIRTPSTEQPIEFLSGGNQQKVLLSRWLLTRPQ 422
             +++G+LR     E+  +A  +  +L +R  S +  +  LSGGNQQKV++++WL TRP+
Sbjct: 375 RISRKGFLR---LAEEFALAREYTERLDLRAASLDTHVGNLSGGNQQKVVIAKWLATRPR 431

Query: 423 FLILDEPTRGIDVGAHAEIIRLIETLCADGLALLVISSELEELVGYADRVIIMRDRKQVA 482
            +ILDEPT+G+D+G+ A +   +  L A GLA++++SSE+ E++G +DRVI+MR+ + VA
Sbjct: 432 VIILDEPTKGVDIGSKAAVHDFMAELAAQGLAVIMVSSEIPEVLGMSDRVIVMREGRIVA 491

Query: 483 EI 484
           E+
Sbjct: 492 EL 493



 Score = 83.6 bits (205), Expect = 2e-20
 Identities = 57/215 (26%), Positives = 104/215 (48%), Gaps = 5/215 (2%)

Query: 276 IAPFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASVL 335
           ++   L + PG++  L G  G+G++   +++ GI   D G  L+ G+P    +P  A+  
Sbjct: 36  LSDVSLSLYPGKVTALIGENGAGKSTVVKILTGIYQPDGGRILVDGQPVPFSTPQAAADH 95

Query: 336 GIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIRTPS 395
           G+    ++     +    SV ENI L   A RG    I  K+  E A   +  +G     
Sbjct: 96  GVTAIHQETV---LFDELSVAENIFLG-HAPRGAFGLIDWKKTTENARALLTSIGAEL-D 150

Query: 396 TEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCADGLAL 455
            +  ++ L   N+  V ++R L    + +I+DEPT  +      E+  L+E+L A G A+
Sbjct: 151 PDHKLKDLGIANKHLVAIARALSIEARVVIMDEPTAALSHKEIEELYELVESLKAQGKAI 210

Query: 456 LVISSELEELVGYADRVIIMRDRKQVAEIPLAELS 490
           L IS + +E+   AD   + RD + + +  +A+++
Sbjct: 211 LFISHKFDEIFRIADNYTVFRDGQLIGDGAIADVT 245


Lambda     K      H
   0.321    0.138    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 570
Number of extensions: 33
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 2
Length of query: 500
Length of database: 510
Length adjustment: 34
Effective length of query: 466
Effective length of database: 476
Effective search space:   221816
Effective search space used:   221816
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources