Aligments for a candidate for gntB in Dinoroseobacter shibae DFL-12

GapMind for catabolism of small carbon sources

Aligments for a candidate for gntB in Dinoroseobacter shibae DFL-12

Align D-gluconate TRAP transporter, large permease component (characterized)
to candidate 3609368 Dshi_2753 TRAP dicarboxylate transporter, DctM subunit (RefSeq)

Query= reanno::psRCH2:GFF2081
         (425 letters)



>lcl|FitnessBrowser__Dino:3609368 Dshi_2753 TRAP dicarboxylate
           transporter, DctM subunit (RefSeq)
          Length = 429

 Score =  330 bits (846), Expect = 5e-95
 Identities = 173/421 (41%), Positives = 270/421 (64%), Gaps = 3/421 (0%)

Query: 3   VVVFLSSLLGFMTLGMPIAFALLLTGSVLMWYL-DFWDVQLLAQNLQAGADSFPLLAVPF 61
           ++ FL   L F+ +G+P+ F LL    +L+W      D+ LL +N+  G DSFPL+A+PF
Sbjct: 2   LIWFLPLFLLFLMIGLPVFFGLLAAPGLLLWLNGQERDITLLYRNVYNGMDSFPLMAIPF 61

Query: 62  FILAGELMNAGGISRRIIAMAQAYFGHKRGGLGYVAIAASVLLASMSGSALADTAALATL 121
           F+LAGELMN GGI+ R++  AQA  GH RGGL +V I +S+L A +SGSA+ADT+AL ++
Sbjct: 62  FMLAGELMNRGGITLRLVEFAQALMGHFRGGLAHVNILSSMLFAGLSGSAVADTSALGSM 121

Query: 122 LLPMMRERGYPLSSSSGLVAAGGIIAPIIPPSMPFVIYGVVTGTSISQLFLAGMVPGLIM 181
           L+P M ++GY    ++ + AA  +I PIIPPS   +IY  V G S++ LFLAG+VPG+++
Sbjct: 122 LIPAMEKQGYTRRFAAAITAASSVIGPIIPPSGIMIIYAYVMGESVAALFLAGIVPGILV 181

Query: 182 GMGLIVAWTLIARRIDEP-KQEKASAAERRRVLVDGAAALMLPVIIVGGLRGGLFTPTEA 240
           G+GL+    L+A + D P    K +  +R +  +     LM PVII+GG+  G+FTPTEA
Sbjct: 182 GVGLMGVVKLMADKYDFPVASAKTTWGQRGQASLKAFFPLMTPVIILGGILAGVFTPTEA 241

Query: 241 AVVAAVYALAVSTLLYRELNWAGLVEVLTRASRTTASVMFLCAAATVSAYMITLAQLPDE 300
           A VA  YAL +   + R L  + L ++L RA  T+A V+ L  AA     +++L+  P  
Sbjct: 242 AAVAVAYALIIGFFVMRTLKVSDLPDILGRAGITSAVVLLLVGAAMAFKTVVSLSYAPQI 301

Query: 301 IAAMLGPLAQDPKLLMVAIMLLMIAVGMVLDLTPTILILGPVLAPIAIKAGIDPVYFGVM 360
           +A  +  L+++P +L++ I LL+  VGM LD  P I+ILGP+L PI +  G+DP++F ++
Sbjct: 302 MADFMLSLSENPLILLLLINLLLFVVGMFLDAGPAIIILGPILGPIFVDLGVDPIHFAII 361

Query: 361 FVLIGSIGLITPPVGTVLNVVGGIGRLRMETLVRGVMPFFLIYLVIVGLLIAVPSI-ITV 419
             +  ++GL TPP+G VL V   +   R+E + + ++PF  + ++++ L+   P+I +T+
Sbjct: 362 MSVNLTVGLATPPMGLVLFVASSVSGERVERIAKAILPFLAVEILVIFLITYFPAISMTI 421

Query: 420 P 420
           P
Sbjct: 422 P 422



 Score = 30.0 bits (66), Expect = 1e-04
 Identities = 24/109 (22%), Positives = 44/109 (40%)

Query: 314 LLMVAIMLLMIAVGMVLDLTPTILILGPVLAPIAIKAGIDPVYFGVMFVLIGSIGLITPP 373
           L  V I+  M+  G+          LG +L P   K G    +   +      IG I PP
Sbjct: 93  LAHVNILSSMLFAGLSGSAVADTSALGSMLIPAMEKQGYTRRFAAAITAASSVIGPIIPP 152

Query: 374 VGTVLNVVGGIGRLRMETLVRGVMPFFLIYLVIVGLLIAVPSIITVPLA 422
            G ++     +G       + G++P  L+ + ++G++  +      P+A
Sbjct: 153 SGIMIIYAYVMGESVAALFLAGIVPGILVGVGLMGVVKLMADKYDFPVA 201


Lambda     K      H
   0.328    0.142    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 571
Number of extensions: 30
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 425
Length of database: 429
Length adjustment: 32
Effective length of query: 393
Effective length of database: 397
Effective search space:   156021
Effective search space used:   156021
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources