Align fumarate hydratase (EC 4.2.1.2); (S)-2-methylmalate dehydratase (EC 4.2.1.34) (characterized)
to candidate 3608019 Dshi_1426 hydro-lyase, Fe-S type, tartrate/fumarate subfamily, alpha subunit (RefSeq)
Query= BRENDA::Q141Z6 (520 letters) >FitnessBrowser__Dino:3608019 Length = 504 Score = 602 bits (1551), Expect = e-176 Identities = 295/495 (59%), Positives = 384/495 (77%), Gaps = 3/495 (0%) Query: 15 TVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEQSPAAKDAIAQILTNSRMCAEG 74 TV+K +D+++++ +LQYI+ YH DY+ L AY EQSPAAK A+ QIL +SR+ A G Sbjct: 5 TVLKPDDIVETVRAALQYIAVYHAPDYLAHLASAYAREQSPAAKAAMGQILESSRLAALG 64 Query: 75 KRPICQDTGIVTVFVKVGMDVRWDGATMGVTDMINEGVRRGYLNPDNVLRASIVSPPEGG 134 +RPICQDTG+VTVF ++G G + +++NEGVR YL+ N LRAS+V+ P Sbjct: 65 RRPICQDTGMVTVFARLGQSAMIAGDRT-LYELVNEGVRLAYLDAGNPLRASMVADPLLE 123 Query: 135 RKNTKDNTPAVIHYEIVPGNTVDVQVAAKGGGSENKSKFAMLNPSDSIVDWILKTVPTMG 194 R+NT DNTPAV H E+ GN +++ +AAKGGGSENK+++ MLNP+ S+ DW++ T+ +G Sbjct: 124 RRNTGDNTPAVTHVELTRGNKLELLIAAKGGGSENKARYTMLNPAASVEDWVVSTIEGLG 183 Query: 195 AGWCPPGMLGIGIGGTAEKAMVMAKESLMDPIDIQDVIARGPKDWIEELRVELHEKVNAL 254 AGWCPPG++G+G+GG+AE+AM++AK ++M+ ID+ D++ARG +EE+RV L+ ++NAL Sbjct: 184 AGWCPPGLVGVGVGGSAEQAMLIAKRAVMEEIDMSDLLARGASTPLEEMRVRLYHRINAL 243 Query: 255 GIGAQGLGGLATVLDVKIMAAPTHAASKPVAIIPNCAATRHAHFTLDGSGAARLEAPSLD 314 GIGAQGLGGL TV+DVK+ APTHAASKPVA++P CAA RHA +DGSG A L+AP L Sbjct: 244 GIGAQGLGGLTTVVDVKLATAPTHAASKPVALVPQCAANRHAKVVMDGSGPAYLDAPDLA 303 Query: 315 AWPKVHWQPDTEKSKRVDLNTLTPEEVAAWTPGQTLLLSGKMLTGRDAAHKRIADMLAKG 374 AWP++ + +RV+++ LT EE+A W PG+TLLLSG++LTGRDAAHKR+ADMLA+G Sbjct: 304 AWPRIEGS-EMAGLRRVNVDRLTREEMAGWAPGETLLLSGRILTGRDAAHKRMADMLARG 362 Query: 375 EKLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDKFTEMMLAQTGLISMIGKAERG 434 E+LPV RVIYYVGPVDPV EAVGPAGPTTATRMD FT ML Q GL +MIGKAERG Sbjct: 363 EELPVSLRGRVIYYVGPVDPVDGEAVGPAGPTTATRMDPFTSDMLGQ-GLFAMIGKAERG 421 Query: 435 PVAIEAIRKHKAAYLMAVGGAAYLVSKAIRSAKVLAFEDLGMEAIYEFDVQDMPVTVAVD 494 + I +HKAAYL+AVGGAA LV+KAIRS+KVLAFEDLGMEAI EF V+DMPV VAV Sbjct: 422 AETVRDIARHKAAYLIAVGGAAVLVAKAIRSSKVLAFEDLGMEAIREFTVEDMPVMVAVT 481 Query: 495 SNGTSVHQTGPKEWQ 509 + G ++HQTGP W+ Sbjct: 482 TQGAAIHQTGPAAWR 496 Lambda K H 0.316 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 783 Number of extensions: 24 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 520 Length of database: 504 Length adjustment: 35 Effective length of query: 485 Effective length of database: 469 Effective search space: 227465 Effective search space used: 227465 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory