Alignments for a candidate for glpD in Dinoroseobacter shibae DFL-12

GapMind for catabolism of small carbon sources

Alignments for a candidate for glpD in Dinoroseobacter shibae DFL-12

Align Glycerol-3-phosphate dehydrogenase; EC 1.1.5.3 (characterized, see rationale)
to candidate 3607214 Dshi_0630 FAD dependent oxidoreductase (RefSeq)

Query= uniprot:Q92LM5
         (503 letters)



>FitnessBrowser__Dino:3607214
          Length = 531

 Score =  570 bits (1470), Expect = e-167
 Identities = 292/519 (56%), Positives = 362/519 (69%), Gaps = 22/519 (4%)

Query: 2   SEQTIFDVFVIGGGINGCGIARDAAGRGYSVALAEMSDFASGTSSGSTKLIHGGLRYLEH 61
           ++  + D+F+IGGGINGCGIARDAAGRG SVALAEM+D ASGTSSGSTKL HGGLRYLE+
Sbjct: 4   TDAKLHDLFIIGGGINGCGIARDAAGRGLSVALAEMNDLASGTSSGSTKLFHGGLRYLEY 63

Query: 62  YEFRLVREALMEREVLWAMAPHVIWPMRFVLPFHKG---------------------GPR 100
           +EFRLVREAL+EREVL    PH+ WP RFVLP+H                       G R
Sbjct: 64  FEFRLVREALIEREVLLRAMPHISWPKRFVLPYHPDMRFEGQTPTSKLLGIFMPWMKGRR 123

Query: 101 PAWLIRLGLFLYDHIGGRKLLPATKTLDMTRDPAGAPLKGLFTKAFEYSDGWVDDARLVV 160
           PAWLIRLGLFLYD++GGRK+LP T+TL +   P GAPL+  F KAFEYSD WV+D+RLVV
Sbjct: 124 PAWLIRLGLFLYDNLGGRKILPGTETLSLQGTPEGAPLEPRFEKAFEYSDCWVEDSRLVV 183

Query: 161 LNARDAADRGARIMARTRVVSARREGGRWAIEIESTETGARETMRARMLVNAAGPWVDRV 220
           LNARDA  RGA I  RT+VV+  R    W IE +  +TG R + RA+MLVNA GPWV  +
Sbjct: 184 LNARDAEARGAEIHMRTKVVATERHADHWRIETQEADTGTRRSFRAKMLVNAGGPWVAEL 243

Query: 221 LSEAVGNNDVRNVRLVQGSHIVVKKKFDDPRAYFFQNPDGRIMFAIPYQDEFTLIGTTDR 280
           L+  +  N    VRLV+GSHIVV K +D  + YFFQ  DGRI+FAIPY+ +FTLIGTTD 
Sbjct: 244 LTGTLRLNVPDTVRLVRGSHIVVPKLYDHDKCYFFQGTDGRIIFAIPYETDFTLIGTTDA 303

Query: 281 DFTGNPADVRISDAEIDYLCRAASEYFSDPVGREDIVWTYSAVRPLFDDGASKAQEATRD 340
           +          +  E DYL   A++YF   +  ED+VWTYS VRPL+DDGAS    ATRD
Sbjct: 304 EHPDPSQKPECTPEERDYLLAFANQYFKQDITAEDVVWTYSGVRPLYDDGASSVTAATRD 363

Query: 341 YVLRVE-NGDAPLLNVFGGKLTTYRRLAESALEKIGETIGEKGRKWTAVSHLPGGDFPAA 399
           Y L+ +    AP+LNVFGGK+TTYRRLAESAL +IG  +      WTA   LPGG+FP  
Sbjct: 364 YTLKTDAAAGAPVLNVFGGKITTYRRLAESALAQIGAQLPVGAGPWTAGVALPGGNFPVD 423

Query: 400 GYDDEVAKLRTRYPFLTASHARRLVRLYGTRAAQLLGNAASEADLGKHFGADLYAAEVDW 459
           G ++ +A+LR  YPFL+A  A RL+R YG  A ++LG+A +EADLG+ FGA L A EV W
Sbjct: 424 GVEELIAQLRAAYPFLSARWATRLIRAYGRDAWEVLGDAKTEADLGEGFGATLTAREVRW 483

Query: 460 LIVQEWALRAEDVLWRRTKLGLKFSRAQTAELEEYMRGA 498
           L+ +E+A +AEDV+WRR+KLGL+ S  +   L+ +M+ A
Sbjct: 484 LMTREYARQAEDVVWRRSKLGLRMSAEEIVTLDRWMQAA 522


Lambda     K      H
   0.321    0.137    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 850
Number of extensions: 29
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 503
Length of database: 531
Length adjustment: 35
Effective length of query: 468
Effective length of database: 496
Effective search space:   232128
Effective search space used:   232128
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources