Align GlpV, component of Glycerol uptake porter, GlpSTPQV (characterized)
to candidate 3609744 Dshi_3127 extracellular solute-binding protein family 1 (RefSeq)
Query= TCDB::G3LHZ3 (569 letters) >FitnessBrowser__Dino:3609744 Length = 579 Score = 716 bits (1849), Expect = 0.0 Identities = 353/573 (61%), Positives = 427/573 (74%), Gaps = 16/573 (2%) Query: 5 AGLLLAMTASAYAGMDEAKTFLDKEVGDLSTLPRADQEKEMQWFVDAAKPFAGMDIKVVS 64 AG ++A + A A A+ ++D+E STL + +Q +EM+WF+ AA+PFAGM+I V+S Sbjct: 11 AGAVIAGQSGAMADEAAAQRWIDEEFQP-STLSKEEQLEEMRWFIQAAEPFAGMEINVLS 69 Query: 65 ETITTHEYESKVLAPAFTAITGIKITHDLIGEGDVVEKLQTQMQSGENIYDAYINDSDLI 124 E I TH YES+VL AF ITGIK+ H ++GEG+VV+ +QTQMQ+G N+YD Y+NDSDLI Sbjct: 70 EGIPTHSYESEVLTRAFEEITGIKVNHQILGEGEVVQAVQTQMQTGRNLYDGYVNDSDLI 129 Query: 125 GTHWRYQQARSLTDWMANEGKDVTNPGLDIDDFIGKSFTTAPDGKLYQLPDQQFANLYWF 184 GTH R Q A +LT+ MA + T+P LD++DF+G FTT PDG LYQLPDQQFANLYWF Sbjct: 130 GTHSRLQLAYNLTEQMAGDWAATTSPTLDLEDFMGIQFTTGPDGNLYQLPDQQFANLYWF 189 Query: 185 RYDWFNDEKNKADFKAKYGYDLGVPVNWSAYEDIAEFFTG--REIDGKKVFGHMDYGKKD 242 R DWF+DE NKA FK KYGY+LGVPVNWSAYEDIAEFFT +EIDG +++GHMDYGK+ Sbjct: 190 RKDWFDDEANKAAFKEKYGYELGVPVNWSAYEDIAEFFTNDVQEIDGVRIYGHMDYGKRA 249 Query: 243 PSLGWRFTDAWLSMAGNGDKGIPNGKPVDEWGIKVDENS-RPVGSCVARGGDTNGPAAVY 301 P LGWR TDAWLSMAG GD G PNG PVDEWGI+++ + PVG+ V RGG NGPAAVY Sbjct: 250 PDLGWRMTDAWLSMAGAGDVGEPNGVPVDEWGIRMEAGTCNPVGASVTRGGAANGPAAVY 309 Query: 302 SIQKYLDWMKAYAPAAAQGMTFSESGPVPSQGEVAQQMFTYTAFTADFVK---EGLPVVN 358 +I+K+ +W++ YAP A F +S P SQG VAQQ+F YTAFTAD VK EG V+ Sbjct: 310 AIRKWDEWLRNYAPPGAASYDFYQSLPALSQGNVAQQIFWYTAFTADMVKPKSEGNNTVD 369 Query: 359 ADGTPKWRFAPSPHGVYWKDGMKLGYQDAGSWTLMKSTPDDRAKAAWLYAQFVTSKTVDV 418 DG P WR APSPHG YW +G K+GYQD GSWT +KSTP DRA+AAWLYAQFVTSKTVDV Sbjct: 370 DDGNPLWRMAPSPHGPYWTEGQKVGYQDVGSWTFLKSTPSDRAQAAWLYAQFVTSKTVDV 429 Query: 419 KKSHMGLTFIRQSTLDHKSFTDRAPKLGGLIEFYRSPARLQWSPTGTNVPDYPKLAQLWW 478 KKSH+GLTFIR S++ H+SFT+RAPKLGGL+EFYRSP R WSPTG NVPDYPKLAQ+WW Sbjct: 430 KKSHVGLTFIRDSSVRHESFTERAPKLGGLVEFYRSPDRTAWSPTGINVPDYPKLAQIWW 489 Query: 479 QAIGDASSGAKTAQEAMDSLCSEQEKVLQRL----ERAKVQGDIGPKLAEEHDLAYWNAD 534 Q IGD +SGA T QEAMD L E + + R+ E A V G GP+L EE D YW + Sbjct: 490 QQIGDVNSGAFTPQEAMDRLAEEMDITMARMQQADEAAGVYGGCGPRLNEERDAEYWFSQ 549 Query: 535 AVKNGNLAPQLKIENEKDKPVTVNYDELVKSWQ 567 +P+ K+ NEK + TVNYDELV WQ Sbjct: 550 PG-----SPKPKLANEKPQGETVNYDELVARWQ 577 Lambda K H 0.315 0.132 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1246 Number of extensions: 48 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 569 Length of database: 579 Length adjustment: 36 Effective length of query: 533 Effective length of database: 543 Effective search space: 289419 Effective search space used: 289419 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory