Align ABC transporter for L-Histidine, ATPase component (characterized)
to candidate 3610800 Dshi_4186 ABC transporter related (RefSeq)
Query= reanno::pseudo5_N2C3_1:AO356_09610 (276 letters) >FitnessBrowser__Dino:3610800 Length = 273 Score = 162 bits (411), Expect = 6e-45 Identities = 91/215 (42%), Positives = 133/215 (61%), Gaps = 14/215 (6%) Query: 45 VVGVNDLSLSIGTGEIFVIMGLSGSGKSTLVRHFNRLIDPTSGAILVDGEDILQLDMDAL 104 V+ ++ + L+I GE I+G SG GK+TL++ LI G ++V+G+ + + D+ Sbjct: 31 VLALDGIDLTIRQGEFLTIIGPSGCGKTTLLKIIASLISHDEGDVVVEGKPVHEPDISR- 89 Query: 105 REFRRHKISMVFQSFGLLPHKSVLDNVAYGLKVRGESKQVCAERALHWINTVGLKGYENK 164 +MVFQ+FGL P K+V+DNV + L VR + E A+ I VGL + + Sbjct: 90 --------AMVFQTFGLFPWKTVIDNVTFPLTVRNFAPAEAREIAMKHIEQVGLGKFVDA 141 Query: 165 YPHQLSGGMRQRVGLARALAADTDIILMDEAFSALDPLIRAEMQDQLLELQKTLHKTIVF 224 YPHQLSGGM+QRVGLARAL+ DI+LMDE F A+D R MQ++L+ + + KT+VF Sbjct: 142 YPHQLSGGMQQRVGLARALSTGADILLMDEPFGAIDAQTRELMQEELMRICQREQKTVVF 201 Query: 225 ITHDLDEAVRIGNRIAILKDG-----KLIQVGTPR 254 ITHDLDEAV + +R+ ++ G ++I V PR Sbjct: 202 ITHDLDEAVLLADRVLLMSRGPGRVREVIDVDLPR 236 Lambda K H 0.321 0.138 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 214 Number of extensions: 11 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 276 Length of database: 273 Length adjustment: 25 Effective length of query: 251 Effective length of database: 248 Effective search space: 62248 Effective search space used: 62248 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory