Alignments for a candidate for liuB in Dinoroseobacter shibae DFL-12

GapMind for catabolism of small carbon sources

Alignments for a candidate for liuB in Dinoroseobacter shibae DFL-12

Align Methylcrotonoyl-CoA carboxylase (EC 6.4.1.4) (characterized)
to candidate 3609096 Dshi_2485 pyruvate carboxylase (RefSeq)

Query= reanno::pseudo6_N2E2:Pf6N2E2_2194
         (649 letters)



>FitnessBrowser__Dino:3609096
          Length = 1145

 Score =  361 bits (926), Expect = e-103
 Identities = 198/446 (44%), Positives = 278/446 (62%), Gaps = 9/446 (2%)

Query: 9   LLVANRGEIACRVMRTAKAMGLTTVAVHSATDRDARHSREADIRVDLG-GSKAADSYLQI 67
           +L+ANRGEIA RVMR A  +G  TVAV++  D+   H  +AD    +G G     +YL I
Sbjct: 7   ILIANRGEIAIRVMRAANELGKKTVAVYAEEDKLCLHRFKADEAYKIGEGLGPVAAYLSI 66

Query: 68  DKLIAAAKASGAQAIHPGYGFLSENAGFARAIENAGLIFLGPPASAIDAMGSKSAAKALM 127
           D++I  AK SGA AIHPGYG LSEN  F  A    G+ F+GP A  + A+G K++A+ + 
Sbjct: 67  DEIIRVAKLSGADAIHPGYGLLSENPDFVDACVANGIAFIGPRAETMRALGDKASARRVA 126

Query: 128 ETAGVPLVPGYHGEAQDLETFRDAAERIGYPVLLKATAGGGGKGMKVVEDVSQLAEALAS 187
             AGVP++P       D++  R  AE IG+P++LKA+ GGGG+GM+ + D  ++A+ +  
Sbjct: 127 IEAGVPVIPATEVLGDDMDKVRAEAEAIGFPLMLKASWGGGGRGMRPIFDPDEVADKVRE 186

Query: 188 AQREAQSSFGDSRMLVEKYLLKPRHVEIQVFADQHGNCLYLNERDCSIQRRHQKVVEEAP 247
            +REA+++FG+    +EK + + RHVE+Q+  D  GN  +L ERDCS+QRR+QKVVE AP
Sbjct: 187 GRREAEAAFGNGEGYLEKMITRARHVEVQILGDSMGNIYHLWERDCSVQRRNQKVVERAP 246

Query: 248 APGLTAQLRQAMGEAAVRAAQAIGYVGAGTVEFLLDA-RGEFFFMEMNTRLQVEHPVTEA 306
           AP L++  R+ + E   +    + Y  AGTVEFL+D   GEFFF+E+N R+QVEH VTE 
Sbjct: 247 APYLSSSQREQLCELGRKICAHVNYECAGTVEFLMDMDTGEFFFIEVNPRVQVEHTVTEE 306

Query: 307 ITGLDLVAWQIRVAQGEPL-----PITQAQVPLLGHAIEVRLYAEDPVNDFLPATGRLAL 361
           +TG+D+V  QI +A+G+ L       +Q  V L GHAI+ R+  EDP N+F+P  GR+  
Sbjct: 307 VTGIDIVRAQILIAEGKSLVEATGMASQYDVQLNGHAIQCRITTEDPQNNFIPDYGRITA 366

Query: 362 YRESAKGPGRRVDSGVE-EGDEISPFYDPMLGKLIAWGENREQARLRLLSMLDEFAIGGL 420
           YR  A G G R+D G    G  I+ +YD +L K+ AW    E A  R+   L EF I G+
Sbjct: 367 YR-GATGMGIRLDGGTAYSGAVITRYYDSLLEKVTAWAPTPEAAIARMDRALREFRIRGV 425

Query: 421 KTNIGFLRRIVAHPAFAAAELDTGFI 446
            TNI F+  ++ HP F   +  T FI
Sbjct: 426 STNIAFVENLLKHPTFLNNQYTTKFI 451



 Score = 44.3 bits (103), Expect = 3e-08
 Identities = 24/67 (35%), Positives = 33/67 (49%)

Query: 582  LAAPMNGSIVRVLVSVGQPVDAGAQLVVLEAMKMEHSIRAPKAGVIKALYCQEGEMVSEG 641
            + APM G +  V V  G  V  G  L+ +EAMKME  I A +  V+KA++      +   
Sbjct: 1079 VGAPMPGVVASVAVQAGASVKEGDLLLTIEAMKMETGIHAERDAVVKAVHVTPAAQIDAK 1138

Query: 642  SALVAFE 648
              LV  E
Sbjct: 1139 DLLVELE 1145


Lambda     K      H
   0.319    0.134    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1597
Number of extensions: 70
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 649
Length of database: 1145
Length adjustment: 42
Effective length of query: 607
Effective length of database: 1103
Effective search space:   669521
Effective search space used:   669521
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources