Alignments for a candidate for ectP in Dinoroseobacter shibae DFL-12

GapMind for catabolism of small carbon sources

Alignments for a candidate for ectP in Dinoroseobacter shibae DFL-12

Align Glycine betaine/proline/choline transporter VP1723 (characterized)
to candidate 3607157 Dshi_0579 BCCT transporter (RefSeq)

Query= SwissProt::Q87NZ5
         (553 letters)



>FitnessBrowser__Dino:3607157
          Length = 519

 Score =  241 bits (614), Expect = 7e-68
 Identities = 160/509 (31%), Positives = 250/509 (49%), Gaps = 35/509 (6%)

Query: 34  VGPFGLDIHNRVFAISGMAIVLFVVATLTFRQQVEPFFAGLRAWLVSNLDWFFLASGNVF 93
           + P G D    +FA++G  I LF    L   + +        A+       ++       
Sbjct: 10  LAPKGAD--KALFAVTGGFIALFCAYALFDIEGLSALVDTSFAFSAKYFGLYWQVLLLAT 67

Query: 94  VIVCLVLIVTPLGRVRIGGTEATPDYSYAGWLAMLFAAGMGIGLVFFGVSEPMSHFSSAL 153
            ++ LVL   P  R  +GG  +TP++    W AM+    +  G VF+   EP++HF S  
Sbjct: 68  FLIGLVLCFLPGSRTLMGGL-STPEFGTFSWAAMIMCTLLAGGGVFWAAGEPIAHFLSTP 126

Query: 154 GGVNIENGVRTDWAPLGGAVGDTDAASALGMAATIYHWALHPWSIY-ALLALGLAIFSFN 212
                           G   GD  A +   +A +  HW    W+I  +L  + L  + ++
Sbjct: 127 -------------PVFGDLSGDVQAQAHAALAQSFLHWGFLAWAILGSLTTVMLMHYHYD 173

Query: 213 KGLPLTMRSIFYPLFGERVW-GWVGHIIDILAVVATVFGLATSLGYGASQAATGLNFLFG 271
           KGLPL  R++ YPL G+R   G +G I D   ++A V G    +G+   Q + GLN L G
Sbjct: 174 KGLPLAPRTLLYPLLGDRALSGPIGLIADASCIIAVVAGTVGPIGFLGLQMSYGLNDLTG 233

Query: 272 VPMTDTTQVVLIVVITALALISVVAGLDSGVKRLSEINMILAAMLLFFVIIVGPTMAILT 331
           +P T  TQ + I+ +  L  +S + GL  G+K LS+IN++LA +LL F+++ GPTM I  
Sbjct: 234 IPDTFATQAIAILALVGLYTVSAITGLAKGIKILSQINVLLAVVLLIFMLVAGPTMNIFA 293

Query: 332 GFFDNIASYITNIPALSMPFERE-----DVNYSQGWTAFYWAWWISWSPFVGMFIARVSR 386
           GFF  +  Y T+   ++M +  +     D  +   WT F+W W++ + P + +FIAR+SR
Sbjct: 294 GFFGGMQVYATHFFDMAM-YRGDAGLFGDAGWLGWWTVFFWGWFMGYGPLMAVFIARISR 352

Query: 387 GRSVREFIICVILIPSTVCVLWMTAFGGTAI-----SQYVNDGYEAVFNAELPLKLFAML 441
           GRS+R+ II + +    +   W T  GG+ I        V  G    FN  LP  L A+ 
Sbjct: 353 GRSIRQIIITLSIAAPLITNFWFTIIGGSGIFFEIAEPGVVSGPFEGFN--LPAGLLAIT 410

Query: 442 DVMPFAEITSVVGIILVVVFFITSSDSGSLVIDTIAAGGKVDAPTPQRVFWCTFEGLVAI 501
             MP   I SV+ ++L + F  T+SDS S VI +    G  +  T  R FW    G++A+
Sbjct: 411 QAMPMGLILSVLFLVLTMCFVATTSDSMSYVISSTMTDG--EPSTAMRAFWGLAMGVMAL 468

Query: 502 ALML--GGGLAAAQAMAVTTGLPFTIVLL 528
            L+    GG+   Q+  V T +P +++LL
Sbjct: 469 ILISTGEGGIGKLQSFIVVTAVPVSLILL 497


Lambda     K      H
   0.327    0.141    0.435 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 860
Number of extensions: 50
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 553
Length of database: 519
Length adjustment: 35
Effective length of query: 518
Effective length of database: 484
Effective search space:   250712
Effective search space used:   250712
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources