Alignments for a candidate for prpE in Dinoroseobacter shibae DFL-12

GapMind for catabolism of small carbon sources

Alignments for a candidate for prpE in Dinoroseobacter shibae DFL-12

Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate 3607991 Dshi_1399 acetate--CoA ligase (RefSeq)

Query= SwissProt::P27550
         (652 letters)



>FitnessBrowser__Dino:3607991
          Length = 653

 Score =  842 bits (2176), Expect = 0.0
 Identities = 401/642 (62%), Positives = 494/642 (76%), Gaps = 5/642 (0%)

Query: 7   HTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGKILDWIKPYQKVKNTSFAPGNVS 66
           +T  A+      I+  +Y  MY  S++ P+ FWGE G+ LDWIKPY KVK+TSFA  +V 
Sbjct: 9   YTPSADFVKNAHIDSSKYSEMYAASVSDPEGFWGEHGRSLDWIKPYTKVKDTSFAHDDVH 68

Query: 67  IKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDD-ASQSKHISYKELHRDVCRFANTLL 125
           ++W+EDGTLN+AANC+DRHL   GD+TAII+E DD A  ++HI+Y+ELHR VC+ +N L 
Sbjct: 69  VRWFEDGTLNVAANCIDRHLATRGDQTAIIFEPDDPAEPAQHITYRELHRQVCKLSNVLE 128

Query: 126 ELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPEAVAGRIIDSNSRLVIT 185
           +LG++KGD V +YMPM+PEAA AMLACARIGA+HS++F GFSP+A++ RI  +++RL+IT
Sbjct: 129 DLGVRKGDRVILYMPMIPEAAYAMLACARIGAIHSIVFAGFSPDALSARINGADARLLIT 188

Query: 186 SDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVVVLKRTGGKIDWQEGRDLWWHDLVEQAS 245
           +D   R GR  PLK N D AL +        ++V+KRTGG+  W +GRD  +H+L+ +A 
Sbjct: 189 ADYAPRGGRQTPLKSNADAALLHTR--DDVKMLVVKRTGGQTTWVDGRDFDYHELMLEAD 246

Query: 246 DQHQAEEMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAALTFKYVFDYHPGDIYWCTA 305
           +     E+ AE PLFILYTSGSTG+PKGV+H++GGYL+YAA+T KYVFD H GD+YWCTA
Sbjct: 247 EVQHPVEVEAEHPLFILYTSGSTGQPKGVVHSSGGYLLYAAMTQKYVFDVHEGDVYWCTA 306

Query: 306 DVGWVTGHSYLLYGPLACGATTLMFEGVPNWPTPARMAQVVDKHQVNILYTAPTAIRALM 365
           DVGWVTGHSY++YGPLA G TT+MFEGVP +P  +R  QV DKH+V   YTAPTAIRALM
Sbjct: 307 DVGWVTGHSYIVYGPLANGGTTVMFEGVPTYPDASRFWQVCDKHKVTQFYTAPTAIRALM 366

Query: 366 AEGDKAIEGTDRSSLRILGSVGEPINPEAWEWYWKKIGNEKCPVVDTWWQTETGGFMITP 425
           A+G + +E  D SSLR+LG+VGEPINPEAW WY   +G  +CP+VDTWWQTETGG ++TP
Sbjct: 367 AKGPEFVETCDLSSLRVLGTVGEPINPEAWNWYNDLVGKGRCPIVDTWWQTETGGHLLTP 426

Query: 426 LPGATELKAGSATRPFFGVQPALVDNEGNP--LEGATEGSLVITDSWPGQARTLFGDHER 483
           LPGA   K GSAT PFFGVQP ++D        E   EG L + DSWPGQ RT++GDHER
Sbjct: 427 LPGAIATKPGSATLPFFGVQPVVLDPHSGAEITETECEGVLCLKDSWPGQMRTVYGDHER 486

Query: 484 FEQTYFSTFKNMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIA 543
           F +TYFS +K  YFSGDG RRD DGYYWITGRVDDV+NVSGHR+GTAE+ESALVAH K+A
Sbjct: 487 FVKTYFSDYKGYYFSGDGCRRDADGYYWITGRVDDVINVSGHRMGTAEVESALVAHAKVA 546

Query: 544 EAAVVGIPHNIKGQAIYAYVTLNHGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLP 603
           EAAVVG PH+IKGQ IY YVTL +GEEPS EL  E+R WVR EIGP+A PD++ W   LP
Sbjct: 547 EAAVVGYPHDIKGQGIYCYVTLMNGEEPSEELRKELRTWVRTEIGPIAAPDLIQWAPGLP 606

Query: 604 KTRSGKIMRRILRKIAAGDTSNLGDTSTLADPGVVEKLLEEK 645
           KTRSGKIMRRILRKIA  D   LGDTSTLADP VVE L+E +
Sbjct: 607 KTRSGKIMRRILRKIAEDDFGALGDTSTLADPSVVEDLIENR 648


Lambda     K      H
   0.317    0.135    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1427
Number of extensions: 66
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 652
Length of database: 653
Length adjustment: 38
Effective length of query: 614
Effective length of database: 615
Effective search space:   377610
Effective search space used:   377610
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources