Aligments for a candidate for puuC in Dinoroseobacter shibae DFL-12

GapMind for catabolism of small carbon sources

Aligments for a candidate for puuC in Dinoroseobacter shibae DFL-12

Align 4-(gamma-glutamylamino)butanal dehydrogenase (EC 1.2.1.99) (characterized)
to candidate 3608023 Dshi_1430 aldehyde dehydrogenase (RefSeq)

Query= BRENDA::P23883
         (495 letters)



>FitnessBrowser__Dino:3608023
          Length = 487

 Score =  312 bits (800), Expect = 1e-89
 Identities = 190/463 (41%), Positives = 261/463 (56%), Gaps = 20/463 (4%)

Query: 38  ETVDPVTQAPLAKIARGKSVDIDRAMSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAH 97
           +  +P T   +A+I      ++DRA++AAR  FE G+WS  +   R  +L+KL + + AH
Sbjct: 29  DVTNPATGEKIAQIPACGPDEVDRAVTAARRAFEDGEWSRLTAVDRGRLLHKLGEAITAH 88

Query: 98  AEELALLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREP 157
            +ELA  E  DTGKP+  + R D+  AAR   +Y  A DKV+GE     S      + EP
Sbjct: 89  GQELAEWEAKDTGKPLAQA-RADMVAAARYFEYYGAAADKVHGETIPFLSGYQVQTIFEP 147

Query: 158 VGVIAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVL 217
            GV   I+PWN+P  +      PALA GN+V+LKP+E + L+ +R+A LA E G P G +
Sbjct: 148 YGVTGHIIPWNYPAQMFGRSCAPALAMGNAVVLKPAEDACLTPLRIAELALEVGFPGGAI 207

Query: 218 NVVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIVF 277
           NVVTG+G EAG AL+ H D+D ++FTGS   G  +++ A   N     LE GGKS  IVF
Sbjct: 208 NVVTGYGQEAGAALTTHRDVDFLSFTGSPEVG-VMIQTAAARNHIGCTLELGGKSPQIVF 266

Query: 278 ADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQ-----QAQNWQPG 332
           AD  DL  A      GI  N GQ C AG+R L++  I D  LA LK       A  WQ  
Sbjct: 267 ADA-DLDAAIPVLLNGIVQNGGQTCSAGSRALIQRDIFDAVLARLKAGFEALTAAPWQEN 325

Query: 333 HPLDPATTMGTLIDCAHADSVHSFIRE-GESKGQLLLDGRNAGLAAAIG----PTIFVDV 387
             L P      LI       V  FI E G +   L+  GR A  A+  G    P +F  V
Sbjct: 326 GNLGP------LISHKQKRRVEGFIAEGGGADAPLVARGRIAASASEAGSFVAPALFGPV 379

Query: 388 DPNASLSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAG 447
                L+REE+FGPVL    FT E  A+++AN + YGL A+VW+ D  R  R+++RL+ G
Sbjct: 380 IEGHVLAREEVFGPVLSCIPFTDEADAIRIANATDYGLVASVWSADGGRQMRLAKRLRVG 439

Query: 448 SVFVNNYN-DGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTI 489
            VF+N Y   G + +PFGG ++SG+GR+K   AL +F+++KT+
Sbjct: 440 QVFLNCYGAGGGIELPFGGMRKSGHGREKGFAALHEFSQIKTV 482


Lambda     K      H
   0.317    0.133    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 611
Number of extensions: 30
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 495
Length of database: 487
Length adjustment: 34
Effective length of query: 461
Effective length of database: 453
Effective search space:   208833
Effective search space used:   208833
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources